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"      #         $       #       %     !                      )       !   &        , !      &                %        (    ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 60cdfd2ace7b472cb420ac574dd7994aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well"K1363_D_4_Asplenium_monanthes_trnhKB_3730_POP7_BDTv3.mobSeqPop7z*R\9_<   [ lr!!%(,O048O7>o>A=<;"9)630-i*%'$!I'B2Tj`mc3 w  y,sT(Q%n7gUI?6-#$/7;8/! 1N^dfoW:4 r DZ$.t;RLcE~}nWF:39-)q%"Q"^_V /cJ'  ; X K e-poJQ, E   f MSjD?[]; ) P T 6OrlPTf8y^@$$# .<=1sT=C~kq9Bz@ #'+4BTi)CNE&m"b8 -KnhI0%0W`Md`&5Z7 %]Y! fG"aZ *d6fb ? 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CE?" 92b(Z6=a<\P4,0%FdR5aQ]TI8cCAC)9aY>D!cK"-2'  [5c8'(2 &b `3Q2=GA\#R/F4`)9 '=M)'Z^0@ JA-M6XUJ/ N?U?X?;Z49SI,` T\17V**DVU.CM+51RI ?!DE"NJ/V _"[F] &:J?; ^O9= R4KK+<3,9V VW<Z=[a(;%ZX`P"D .!NC ;*)G)XG/.%"aF=BW2MX(WO*cc ;^0 Q6",?FGRMGD46.4LQ!Ga)*,QON,` F)Q!GXK(C4a'%Y2 Y#1 /b >E9:Z4%(_W9,\21ZZ5HK;C8-?< /=KdU6RU%:M ,)@I8`H,64xhg Vt\ZaR  z 5`TR e9q3xT!h.v /Pk@rjwqOfx5, !*I"[F/6z"\x x#Ks00KP,PH"BT\-1Ik5UShg~T g6dsKSZ" ^Fmk2#I(ECb3Z <|mP ?YI/4"^^j&mRn#L Yz>4(<qC0Zf|t?RpGUuR (5u!Vv,]Yg^]qi#B{4"yrAKXl"Sc;*[#f# )}n%jAh{ r1,ZYeM@tMk   0pue ^( (CGhH+SFmBbNv`5]Ya9=0$((*+ $&<9 #]<<'0~]*RTK0?K<7('  '!@D !               &   #06     ''-+ (  )-&()/ "7!'?!:2063-!,4)$99,6$$$2$&2/Bp%(*EI0')#'ZL:i$ ppg|X|pW&ppPp*c2p+d0{CEJZBx/%Y@@fi=-Yq7FM6\pud)dndd*X:LHPm*(rxp"`q2>'|ZdFwd&RrQG8E:828X;2K)7M='dpRxp8q8&4'8 "y84jl0fpPeppoOCa E'l KPdEcpvzdjjQ/2C O`am-2:2YY % j[s e;RyR[/wnYDYA(H$+'1)"B`;?e$4ElI^x sia:,i-LJ\*^D!3 677--78`#S1:mQtjQM*^8' &-,)- ))A@0)%L':2 B1$&5>> ``OP`ykGJ* $! %+(5,+M'-,%(W.B336C4.,5  $&/)()(1,-U(%:9$*37D&0$" q2<%*#81!(" )&+ 2 4$"%0"A#( * - G<1>$+!--6/&$3 28(EN!!P8&ar%"!/9$HH@<N96@J6AVPl+>FUCM--(/DcI@<088GJ1t> bU|1LQf4if%O7J9B&^qRKC/)fd*XFD>R[CZ~Ia 1L<'FGGGAGGGCCCCCCGGGGCCAAACCCATCTTGAAAGATGCGTGTTCTGTAGTTAAATAGGACAAAAAAAGTGGTACACCGCCACATCCTCCCTTAAAGGAGGCCGCTATATTGGCTCCGCGAATTGCACACCCTTATAAGGACTGTATGGAGATGACGAGACTATAGTGGATCCCTGGTAAGAAGGATGGTATCTCACTTTCACCTCCTCCCAAAGAAAATGTTACAGATCGCGAAGAATTCGCCTAGCCGAGGAGAATAAGTCATTATCCCATTCATAGCAGACAGAGACTACGGAAGGCAGTTGGGACTTGATGGTAGGTTCATAGGATCGCGGTAATGAGACCAGGAGATATAGGCGCTTTCATAAGGGCTATAAGGCGGAACAATTAATTTGGGAACCTATATTCTTCTCTCGAAAAGTGAGATCCCGGGTACCAATGTTCTTTGTGTACCCAAAGGGAGGTGGCCCGTATATTAAGAGGGGTGGAAGGAAGGCCAAGTCTTCAGGAAAGGTTTGGGCTTTAGTTACATGCGTACACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:56:03 -04:00AEPt3AEPt3APFN APXV2APrN ×APrV1APrX}}àARTNASPFASPtASPtB1PtB1PtBCTSUBufT"DiCTID;CTNMACTOwYCTTL dCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D&DATA"DjDATA (QFDATA (QF76DATA (QF|DATA (QFDCHTDSamDySN EDye#DyeNPDyeNUDyeNZDyeN_DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!$HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~S}LIMS!!mLNTD2LsrPaMCHNMODF ÎMODL3730NAVGNLNE`NOIS+OfSc(P1AM<@oP1RL<<3P1WD<DP2AM<M#P2BAQ_P2RL<HPBAS-aPBAS+CPCON:PCON7PDMFPDMF+'PLOC<3PLOC</PSZE`PTYPaPXLBRGNm3RMXV1RMdN RMdV1RMdX&RPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%+SCAN3SMED SMLtSMPL##SPACAh*SPAC+ SPACAh*SVER4.0SVER TSVER,,®Satd(^Scal@Scan3TUBED4Tmpr<UserphARphCH+>phDYbigphQLcphTRphTR'This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ×APrV1APrX}}àARTNASPFASPtASPtB1PtB1PtBCTSUBufT"DiCTID;CTNMACTOwYCTTL dCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D&DATA"DjDATA (QFDATA (QF76DATA (QF|DATA (QFDCHTDSamDySN EDye#DyeNPDyeNUDyeNZDyeN_DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabcaFVoccPFWO_GATCFeat//c!GTyp!!$HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~S}LIMS!!mLNTD2LsrPaMCHNMODF ÎMODL3730NAVGNLNE`NOIS+OfSc(P1AM<@oP1RL<<3P1WD<DP2AM<M#P2BAQ_P2RL<HPBAS-aPBAS+CPCON:PCON7PDMFPDMF+'PLOC<3PLOC</PSZE`PTYPaPXLBRGNm3RMXV1RMdN RMdV1RMdX&RPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%+SCAN3SMED SMLtSMPL##SPACAh*SPAC+ SPACAh*SVER4.0SVER TSVER,,®Satd(^Scal@Scan3TUBED4Tmpr<UserphARphCH+>phDYbigphQLcphTRphTRrIvMa3o';E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(`"]drh(>LO|PVYWEI59{);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilVL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitG`zKHesJmbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhA1!T]{w[Om#Np#04&Yc4_OYx|Y?&D|4YJI*ys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LphDYbigphQLcphTRphTR