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"          d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y~|{xwutqqnljhgecOOOEGGddd{{{   $$%,--555===EEELLLSSSZ[Zaaahhhnnnuutzzz~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 14e68995ca4547c6864e8b0af79e8eb9Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_16-56_19703130Seq1012010120_Lehman_K1355_trnhjmadera96-WellK1355_E03_trnhKB_3730_POP7_BDTv3.mobSeqPop7z',4CYu9Wv0]4mTI(b(AMME7%k+On%Q S!-JkpIwN%hN9(  }sle_ZUPMJIIIIIHGDB?=<<=>>>===>?ABBAABDHNU^iuL+?z1uu2A|%^1  3c>+xB w  { [ W+^oVB2$/Wh3Z|.l8jA 6gDU @:Prg_YTPLHFDBA@@@ACDDDCA><952-)%" !)9Rv2}y:a6&-*8@fPB:4/+)((*,--+)'&&'((&$!   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J@FAffAR˒AKB.bcpKB_3730_POP7_BDTv3.mobtermGAGGTGCGCTTGACAATTGTCGACTTGGCTCATCCGCCCTCTACTATTATCTAGTATTACTATATTTTTCCATTAACATAAGAAAAGCATTTTCTTTCTTTCTTATTTAATAAAGAAAAGTAATAAATAAGCAAAATTGTCATTTTTATCTATTTTCGATTGAAATTGAATTGGAAATAAAACTTCATAAAAGATTGGTAAAAGGATATGATATAGAAATAGAAATAGAAACCTATAATATAAATGAATACAAAGAAAAAACACGCAAATCGAACCAAACTTTAAAAAGTCCTTGTTATTTTTAAAGAAACTATGTAAGGCAAATAGTACTAAATGCAAATAGTACTAAATAAAAAAAAGGAGCAATAACGCCCTCTTGATAAAACAAGAGGGAAGCTATTGCTCCTTTTTTAGTTCAAAAACTCCTATACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTATGAGCATTACGTTATTGCATAACCAAAGGAGGTGCGCTTGACAATTGTCGACTTGGCTCATCCGCCCTCTACTATTATCTAGTATTACTATATTTTTCCATTAACATAAGAAAAGCATTTTCTTTCTTTCTTATTTAATAAAGAAAAGTAATAAATAAGCAAAATTGTCATTTTTATCTATTTTCGATTGAAATTGAATTGGAAATAAAACTTCATAAAAGATTGGTAAAAGGATATGATATAGAAATAGAAATAGAAACCTATAATATAAATGAATACAAAGAAAAAACACGCAAATCGAACCAAACTTTAAAAAGTCCTTGTTATTTTTAAAGAAACTATGTAAGGCAAATAGTACTAAATGCAAATAGTACTAAATAAAAAAAAGGAGCAATAACGCCCTCTTGATAAAACAAGAGGGAAGCTATTGCTCCTTTTTTAGTTCAAAAACTCCTATACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTATGAGCATTACGTTATTGCATAACCAAAG-3Iay -9GOZm "6?PYgox ",:CQ[gu #0>IT_ju ".:DP]ir~ !-9EQ\hu ,9CO[hs*6DOZgs    ) 4 @ L X d q |   $ 1 ? 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Y!+!e}\f] >[%ZJKVJv Z~0kgd/I\/YMT`j rtgs_baXM\NGJ2;EG8@EA:15#$-/30/"$!$"" ":'                                    % & #   $'!,),1 !/)(1' -.) -- #4"*/2-386!3).I69667B'E6*&2;D:8;8*<LD<2?I.O7F3?B9VC3?8U"BAEG<NECgZ1FEDD4T7-;71(663m<r<<Fa<<(L#p"z< ;U|M^nppbKH]8$,EJCpdLpspC!g>SH!]-7zB8,hMp2lqM|Op1X]q`=r4IN?u8 UHpdG1JZW)6-Y h i3>pO}A)IupKpr_Bc,qY(ZIpp,SpDb, b5_Dcvv %3]' w" W#^ We9S  !v<H#ue)5 V'03(Z; 5J ) [_S#  $$ ," (8   +$ ' "  *   $  ( A 4&!"") !(  );  /  !$($,.,7B$,:I7*+#$%"*-) 3+04/,2:;;1("'6A!$3/-H/E~3(o?:h&T *<2%)+6#2%"4\812P^6AS,N05Q5V'%19K!LJ47&+rlAGACGCGCTCGAGGGGCCAAATCACGTCGAACCTGCGATCTATAATAATAAATAATAATAATATAGGCCTACAATGACATGAGGCCCTTGAACGCCCGCGGGCATAAATGATGCCGGCGTGTTATTCCCCCGCGGGGCCGTTGCCCCTATCTAGGATCGAGCGGGAATGGAGTTGGGATCTGACCGCCTTTCAGAGTTGTTTGCCTACACGCTAGCGGTATTGTTCGTGTGGTCCATTCTGGGGGTGGATTCCTAGGGGTTACACGCTTATAGGAGCCTAAAATTGGGAGTTCCCGGTAAACCTCGAGGCACCACGTGAAGGGGAGAGTTCTTTCCGCGTCCAGTTCCGTCGGGGGTGGATGAGCTATGACGTTCTCCTTATTTTACTAGAAAGGAGCTACAGCTTCAAAAATAAGACGGGGAATTCTATACGATCAGAGTTTTATCATATTCAACTATAGAAAGAGCCTCCATAGCCACTTAGTCTAGAAAGGTAATAAGAGGAATTTGGCAGTGCAGCAACCCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 18:27:24 -04:00AEPt3AEPt3APFN 3APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSUBufT&LCTIDܓCTNMܙCTOwܱCTTL ¼CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&LDATA&L"DATA&LnDATA&LDATA (Q<DATA (Q74DATA (Q,DATA (Q$DCHTDSamDySN Dye#DyeN¨DyeN­DyeN²DyeN·DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT >EVNTJEVNT]FWO_GATCGTyp!!|HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~TaLIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS-$OfSc<P1AMAP1RL=P1WDEP2AMN7P2BARSP2RLJPBAS/ePBAS-WPCON;PCON9PDMFPDMF-;PLOC5PLOC1sPSZE`PTYPܹPXLBRGNm܋RMXV1RMdN uRMdV1RMdX~RPrNYRPrV1RUND  RUND  RUND  RUND  RUNT 8'RUNT 8RUNT .RUNT Rate pRunN""iS/N%-SCAN3SMED SMLtSMPL SPACAf)SPAC-4SPACAf)SVER4.0SVER USVER,,Satd<^Scal@Scan3TUBEE3Tmpr<UserphARphCH-RphDYbigphQLcphTRphTR*This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN 3APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSUBufT&LCTIDܓCTNMܙCTOwܱCTTL ¼CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&LDATA&L"DATA&LnDATA&LDATA (Q<DATA (Q74DATA (Q,DATA (Q$DCHTDSamDySN Dye#DyeN¨DyeN­DyeN²DyeN·DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT >EVNTJEVNT]FTabdEFVocd4FWO_GATCFeat//dGTyp!!|HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~TaLIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS-$OfSc<P1AMAP1RL=P1WDEP2AMN7P2BARSP2RLJPBAS/ePBAS-WPCON;PCON9PDMFPDMF-;PLOC5PLOC1sPSZE`PTYPܹPXLBRGNm܋RMXV1RMdN uRMdV1RMdX~RPrNYRPrV1RUND  RUND  RUND  RUND  RUNT 8'RUNT 8RUNT .RUNT Rate pRunN""iS/N%-SCAN3SMED SMLtSMPL SPACAf)SPAC-4SPACAf)SVER4.0SVER USVER,,Satd<^Scal@Scan3TUBEE3Tmpr<UserphARphCH-RphDYbigphQLcphTRphTR[4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95E3&*L!g3]CUST53m9\RwEbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHP)$j f+{Ga`!d_|,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"+%3N,'BDD"$m&*\QX4R:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NEVNTJEVNT]FTabdEFVocd4FWO_GATCFeat//dGTyp!!|HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~TaLIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS-$OfSc<P1AMAP1RL=P1WDEP2AMN7P2BARSP2RLJPBAS/ePBAS-WPCON;PCON9PDMFPDMF-;PLOC5PLOC1sPSZE`PTYPܹPXLBRGNm܋RMXV1RMdN uRMdV1RMdX~RPrNYRPrV1RUND  RUND  RUND  RUND  RUNT 8'RUNT 8RUNT .RUNT Rate pRunN""iS/N%-SCAN3SMED SMLtSMPL SPACAf)SPAC-4SPACAf)SVER4.0SVER USVER,,Satd<^Scal@Scan3TUBEE3Tmpr<UserphARphCH-RphDYbigphQLcphTRphTR