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PQRSTUVWXYZ[\]^_`abuvwxyz{|}~PQRSTUVWXYZ[\]^_`abuvwxyz{|}~m2<,,,III^^^{{&&&999DDDXXXaaavvv      ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRtz{|{z{yyxwvtsrqoomkjgfdca^\ZYVTTQN NNFFFcccyyy   (((00/888???FFFMMMTTT[[_aagggnmmsssyyy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ff73209383494681aad1df4a185ec377Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_22-23_07013130Seq16745134745_134745mfagan96-Well%K1316_H2_Tillandsia_punctulata_trnH05KB_3730_POP7_BDTv3.mobSeqPop7z-Kf}1c,BM |[j  "$&(*+ +u+v+=******++K+, ,A,#+*](&$]"IkE N } @ 1 N x  NPn^"!_uhC] ! 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ZG&vQ<X3~^&^k$0YQ7 4OaA}SR9Hp5In"MK%^J )VV]Rm; 9 dQeF }]D+*43/rb   a.q}fgG}U = .7r5x/* ` `)L4bTYPxheSM<*K6UB9FC:X!"'K1,D[yp+}           )    ' $   .?~    1)pp]I68?!php&I@O.1v,O pxV;[1 Rz b 0  PTxdhHf89v{noVL?SJBP<8ePX;JZP]%.J jw:=8~)w#S_:Hc;CvqQ_6|&(Ryn &4+ *CtcC)>:t(:x/Y@: <d$GM{QZ$i#J{2.M `]<5VwUl*i.T75 Q!^T }zUb>1d2CX,6,;&,nF    m 6 G;EI"!w"]!c/J( 9(N<&< (a %,$kg"`%{q |6v3eyx,S}<<?()wZKk2K3*E>+/KG&.+**E5,$/.'" &),'0<''&/* !9)<6U650$$"!. 8:0B"+4"311 [am_>=I0BW}Y#~cqSmG5WqZK9zX*}@gPTP^iS;BxG)hdxiYU6u)a,HB<2/kc8WYt_rUc@>qBHj2vw8rxqL2TNJQQVs3 ' ]jFFNk|H25'^)kODfEv5N@b^ukgeYKHKa?U>FW`9<$8:r[_[c;aRJ;_-=BD(atbDV^bjYe{g|SVlB\Y_JuW]b^]9>[Xo<#QJL.L+kBPVWCG>e":7:#:RI|Fr=>:Q ,eu2y}*l+FTGGATGCGCCGCTTATGGGCCCCAGTTAAGTAATAATATTACTTCTTAACTTGACTTTACTACTGGACAGTGAGGCCATGTCCGAACAAAAGCCCCCGAAGGGGATACCACGTTCTTCGGTGCGCAGGTAGGCAGCCCGTATTTGGGCCTTCGGGTCCCGGTTCGTTTGTTCTCCCTGAGTTCTGTGGTGCTCAGATACCAGCTTCCCCTCTCTTACTCCCGAGGATTATGAGTATATATTTAGCACACAGTTGCACACGAGCCTCTGATATACTATAATGAGCTATCTACCGGCGACCTTATGTAGTTTAGGTGAGTTGAGGGTGAGTGCCGATAATGTGTTTATTTCACTATTGAGCAGAGTGTATCTTTACTACCCTCCTCATTATAGCACGAGGTGGGTTGTGGATCTATCTGGGACTCTCATACATAATTACGACGGTGTAATATTTGAGTCTATGTAGGTACTACAGCTCCTCGCTAGGGGTAAAAAATAAATGTGGTCAATACAGTACTACACACCAACAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-01 00:10:37 -04:00AEPt@AEPt@APFN W0APXV2APrN 3JAPrV1APrX}}3SARTNASPFASPtASPtB1PtB1PtBCTSmBufT MCMNT 3 CTIDLCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (yDATA ={PW9DATA ={P҉DATA ={PMDATA ={P)DCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2yEVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~kLIMS!!3LNTD2LsrPaMCHNMODF3:MODL3730NAVGNLNE`NOISDOfScN8 P1AM"Y^P1RL"U<P1WD"]P2AM"eP2BAiP2RL"aPBASFPBASDPCONS+PCONQPDMFWPDMFDPLOC"LPLOC"HPSZE`PTYPMPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%DySCAN@SMED SMLtSMPL&&VSPACAhSPACDSPACAhSVER4.0SVER muSVER,,2MSatdN8Scal@Scan@TUBEH2Tmpr<UserphARphCHDphDYbigphQLcphTRphTRY䁅This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN W0APXV2APrN 3JAPrV1APrX}}3SARTNASPFASPtASPtB1PtB1PtBCTSmBufT MCMNT 3 CTIDLCTNMMCTOwMCTTL 3CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (yDATA ={PW9DATA ={P҉DATA ={PMDATA ={P)DCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2yEVNT 2EVNT2EVNT2FTab{FVoc{FWO_GATCFeat//{GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~kLIMS!!3LNTD2LsrPaMCHNMODF3:MODL3730NAVGNLNE`NOISDOfScN8 P1AM"Y^P1RL"U<P1WD"]P2AM"eP2BAiP2RL"aPBASFPBASDPCONS+PCONQPDMFWPDMFDPLOC"LPLOC"HPSZE`PTYPMPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT  RUNT  RUNT /(RUNT  Rate 2RunN""LS/N%DySCAN@SMED SMLtSMPL&&VSPACAhSPACDSPACAhSVER4.0SVER muSVER,,2MSatdN8Scal@Scan@TUBEH2Tmpr<UserphARphCHDphDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`D5^ivoQ.HNWnAPU\ +6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f. uX#0gLjn%OM66ECxt~P"xooe?+kT#*7R&[hHQbS9YH|O[nYnuPQh^5Q1V?yRl<`QV-@[3>>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["#87zFl@xK/h+$l*L|6lxg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39Nm%Vl#a! x]I4'PNs-}/WY%Ol],lp^^Jg}OK9]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0