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EEE.>>VXXlll}}}      '''...555;;;AAAGGGMMMSSSXXX^^^ddciihnnrssxxx|||~~~~}~}}}}}}}}}}}}}}}}}|}}||}|||||||{{|||{{{{{{{{{{zz{zzzyyyyyzyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyxyxxyyxyyyyyxxyyyyxyyyxxyyyyyyyyyxyyyyyyyyxyyyyyyyyyyyyyyyyyyyxyyyyyxyyyyyyyyyyyxyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyyyyyyyzyyyyzyyyyyyyzyyyyyyyyyzzyyyyzzzzzyzyyyzyzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz{zzzzzzzzzzz{zzz{zz{zzzz{zz{z{{{z{{{z{{{{{{{{{{{{{{{{{{||{{{{{{{{{{||||{||||||||||||||||||}||||||||}}|||}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~~}}}}~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b62965aac3904cf2b0c7b256546b98a1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-01_17-15_01193130Seq1282612826_Steier_K1337_trnHjmadera96-Well K1337_D1_trnHKB_3730_POP7_BDTv3.mobSeqPop7z 0AOYajv!;Tk)Pw*^,X~ %;LTSH5qB[TxP-  )4@KQROJFDC@8)lWD2$!,8CLQUX]ftygVF6&  '.5<BJS_n@80mY k  ? B  oG"q@h$ D[% E N E . g H.{^H3udR@/+Fc*T|vS1nUC71.,(# vkaVLD<60,(%#! !$'+/268:;;=@GR`q~tlf`YQICAAEJORSSTVY\__]ZURQSUY[[ZYX[bm|vbP>-  +3662-'!        $'('%!                                 $$                                                                            jlou |tme\SJC>951,'"      !$')*(%"!!#&'''%$$$$%$#"#$&)**)&$"! #&'((()+/38;=?BFNYgx %?Wl~wqnlmou  2CR^glmjcWF/AjrGpM1  7]/?M) ) U,}i6HyWA3*#       /9<8/!xcTKGHKRYbksyzskaWNFA>=>???>>>@DIOSWY[^afkpuz $;]I <_wh- cx\I>9778863/-,-17<@AA>952000110.+'#                              "$%$"! !#&*.010.,++,/121-'!$,354.%                                                                                                                                                                            "%)-26:>BGLQVY[[YXWWWWVTPLFA;60*%        #%%#  +Ed,sY[L{xvvvwwwvvvy}   wmd\VRONNQU[bjr{}xvvy~|rg\RH?82-*(''(*-16;BIQZbjqvz}|xqh]PB5)   $'))&#      $&&#   #$$"  #'('#"$$!                 #(+*% #)+)$   "&'%           "'+-,)&"!#$&()+-0257899853/+(%###$$#!             !     !   "%%! !"                   !!     &+-*$ #(*(# ""                                                                                                            $&''').4;BHMQUZ_emu~ (1:ERap}~|rg\QD6' +=RhkP4{qljlnppmid`\YWTQNKIGGHIIIIIJLNQTY_goy*US}Nz^G4'#.<Of*T~  uw z 3 ^ w u S"{6a.?(Jym 3 ~ %#@  gD$zm`TJ@6, uld^ZWWY\`ekrz}unhb]XTQPPRV\ckt~ xpic^XRLGB>:63/+'#  "%(+/1355431/-,+***)('&&'),/234421/-+(%"     !     "#"  "&+2:CNYdnuz}~~~~{wsme]TLE?94.(#        !#!   !    !                                                          @AHA4PAKB.bcpKB_3730_POP7_BDTv3.mobtermAAGTCGCGCGTGCGACATCAGTATCATCTGCTACAAGGCTCTCTTTTCTTGTTCTGTTTTCTTTATTTTTAAATGAACTTAAAAAAAATAGATTCTTTCTAATAAAGAAAAGTAATCAATAAGCAAAATTTACATTTGTCTCTTTTTTCGATTGAATTTGATTTGGAAATAAAACTTCATAAAATATTCGGAAAAGTTTTTGATATAGAACCCTATAATATCAATGCTTCAAAAAAGGGAAAAAAAAAAAAAACAAGGAGAAAACACGCAAATCGAACCAAACTAGAAAAGGTCCCCGGTTTTTTGAATTAACTTTTGTAGGGCAAATTTTCCCAATTGTGAAAAAGGACCATAAAGCTCCCCTTGTTTTTTTAAGTTGAAGCCTTTTCTTCCTTTTTTCCTCCGTCTTTTCCATTCCAATCGGCCCCTTGTTTATCCCTTTTGTCTAGGGGGCTTTTAGGGGGGGGAGGGGTGTAGAGGGACATTTGGGGGCCTTTGGTCAAGGGTTAAAAAAAAGGGGGGGTGTAAGTCGCGCGTGCGACATCAGTATCATCTGCTACAAGGCTCTCTTTTCTTGTTCTGTTTTCTTTATTTTTAAATGAACTTAAAAAAAATAGATTCTTTCTAATAAAGAAAAGTAATCAATAAGCAAAATTTACATTTGTCTCTTTTTTCGATTGAATTTGATTTGGAAATAAAACTTCATAAAATATTCGGAAAAGTTTTTGATATAGAACCCTATAATATCAATGCTTCAAAAAAGGGAAAAAAAAAAAAAACAAGGAGAAAACACGCAAATCGAACCAAACTAGAAAAGGTCCCCGGTTTTTTGAATTAACTTTTGTAGGGCAAATTTTCCCAATTGTGAAAAAGGACCATAAAGCTCCCCTTGTTTTTTTAAGTTGAAGCCTTTTCTTCCTTTTTTCCTCCGTCTTTTCCATTCCAATCGGCCCCTTGTTTATCCCTTTTGTCTAGGGGGCTTTTAGGGGGGGGAGGGGTGTAGAGGGACATTTGGGGGCCTTTGGTCAAGGGTTAAAAAAAAGGGGGGGTGT 9Pah|,7DLUdoy 4LTcr2<GS`t #-;DOZfp~*7BLXaoz %0<IVamx .:DR]is(4BNYep|  # / : F R ^ l x    ) 7 D P [ h t    % 3 > J V b m z    ( 4 @ K U a m z   * 7 B O \ h s ~ "-;IT`lw*6BLZdr $0<ITamx,6EQ]jw$1?P_kw,8DQ]hs'2?MYep~)4?MXdp})4@KW`jt  ,7DNYcmz &/:FS_ly 9Pah|,7DLUdoy 4LTcr2<GS`t #-;DOZfp~*7BLXaoz %0<IVamx .:DR]is(4BNYep|  # / : F R ^ l x    ) 7 D P [ h t    % 3 > J V b m z    ( 4 @ K U a m z   * 7 B O \ h s ~ "-;IT`lw*6BLZdr $0<ITamx,6EQ]jw$1?P_kw,8DQ]hs'2?MYep~)4?MXdp})4@KW`jt  ,7DNYcmz &/:FS_ly           &      1) ('  ""%'""$ !" "  """"'"    $""+'""               ""'   "'              ""        !  """      $        "+ +            &      1) ('  ""%'""$ !" "  """"'"    $""+'""               ""'   "'              ""        !  """      $        "+ + U:V6\K<D L G%c4ca F 5# ZKR`Q. *>?R_'! !*/^^[*.H%K6= <+AC4" K(LYOO2-M b&_1J'WG24"B?P+\O3TF Lc7 ]+"4bC(CG#2(NO()=N(]]6=EZX'27#.9ZQ+9OZ[%2U2_3UUU7A8WNUH$*1E 5\ KL$= '$L>L* )>T();2042^5]\=:. -C<27M"cR&[<b&=T&!6P\SU K2O8 U^% &8\WGW2C&R>Y^Z#ZO!;J/cN8 BG*XI/C!a!C2!C1?R8C!>S2CGA"C$R$#LQ1L42$ b@$-O@ U%>=$G2B@<.40,<^_SZU2_DD(^1^]cNROUM5SJKKL<![2 (ECR;2XU_IM2('K\%)2JJ"?Y3M(5 YD/T0I.u&G@c"S%h 6EcAX|Tjol2aj6TD9v;2y>>~o+ho?W}k~Ie:#(I{syvB!Pp! :m3_~\ Dfc 9AR#wfF9EcO;P#Ot'Bav[ :1/OE^\FW)!%6/XM&h0uOsSB4aMjFeq>Px *X(S6erY*cSA .%GS/`> )n  msE?U&U.%g^M%$5 - w~%.C7V ;.$nh)aa&  d\7cu /b^iEZ6M.XVp p:G( ^jL 3y (C&c4GEKAUB`NLMfWiPn$9S pPs"ld/>ohB3$" 8#)2' BPKb45NN>  n>D/Oq- t=!O 8'1)* %.$1> >;%,pjl"pK1kz-Rz%HzgcUbV$NpZu0uxgI]xozuX<g)m'{%6u[08GE^O" 5ONx, 94e@Crd+vI}NMTuyW FZua6#As1 O,@3B>*v&p]g}&n,Q X,A22H9P@"< eh6L{5-X8o!Yo R=o&1CKPY,up;%4X#d;$c)*#$x& :n^d>p/,I"H&O&5N<8pJB*yI-Ts<0 ]^aG?[ _(v)%xu?TnXZ+Z>c^\Oh{ \+XO*%at+4j`p wa2xjS]P7jrb:y!@uy:AIUV<\FeNM36pFsp h}tp D>Nz4J(;E8k$A  EjbS_%k&o{;x48e^#(2 _ (:Z7_q\B^-aTaF&.8}E<C9 D}/a`p [U~ SqL.1'89:qO$Ht!K = #nP;om,;c+wI<A8`\sLGGCGGCGCGCGCGAGGCCACACCATTCTGTGATACCACGCTAAAAAGTCACGATATGACCGCACTACAAATGCGCCCACCCTTTGGGTGCTTCGTGAGTATTATCCTCCCTTATGGACGCCCCTGTGGGGGCTTCGGCATCTAAGGGCTATAGAGTACGCTACGCCTTTGCTTGTGCATAGCGTATGGGCATGGGCCCCAGATAGATAGGGGTCTAGTGTCATTGTTAAACCGGGGAAAGCTGGCGGGTTTTCACTCAGAGTTCTCGCGCCTGGATCTATTTTAGAGGCCAACTTTGTTAAAACGATCCAGGACAAGTCTTCCACCTGGACATACCACAAAGGTGGACGGGCATGCCGCTTTTGGTGCAAACCCGAGGCGCTGTGACCGCCTTCCCAGCGGCTAAAAAACCTGCACAATTGATCAAAAAATCCGTATGGCGAGTGAGTAACACTACCCTTTCCCTTTGCAATCCAGACATACATAACTAAACTTGGCTTCTCCTCCAACCCCCCCGCAATCAACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-01 18:46:25 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTS:BufT'NQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA %K<DATA %K<0 DATA %K<{EDATA %K<ƁDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~9LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM&P1RL"hP1WD*P2AM2P2BA6P2RL.PBASPBASPCON ZPCONLPDMFPDMFPLOC0PLOCPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT . RUNT %RUNT ,Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAxԍSPACSPACAxԍSVER4.0SVER :SVER,,Scal@Scan3TUBED1Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTS:BufT'NQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA %K<DATA %K<0 DATA %K<{EDATA %K<ƁDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabHFVocHFWO_GATCFeat..HGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~9LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM&P1RL"hP1WD*P2AM2P2BA6P2RL.PBASPBASPCON ZPCONLPDMFPDMFPLOC0PLOCPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT . RUNT %RUNT ,Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAxԍSPACSPACAxԍSVER4.0SVER :SVER,,Scal@Scan3TUBED1Tmpr<UserphARphCHphDYbigphQLcphTRphTR-'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWir;-rVN:!PY,4wK;mV~uq_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{nA&|#pdZ8'%{ Tv*PK@|Q$OEHbX\"B-8'i2{&[y3( ]'ngg61C8dk7YZFU?-4$&LkfG;7oF`|zvn[Y<,h`+m&wE6Gz zF/"Wtixy$|FRrtlqaxkabCDk$OE-`{siO}L6iyZnsz3P<}u^ZqNX+9_e V&&OE6OLo X:QJf9/qwAxe<][#m*ZY8|k_R.AQ>UR6? sdWUW>|=);C]MfkltH0j],P2%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tu#,xHL?iCB0'~nRnX@EV$2kE}:>&ZgL7:tl|y\@for\O:YG7Q$$OWUI2c+-@[3>>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#