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  .   %                "              "    "     " "&         '             #  " & #$     !#  *  # %   '$  &   HIJHIJ{",,III^^^{{{&&&999DDDXXXaakvv      **333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRSTTMMMlmm     **)333==<FFEONNWWZ_`fhgooowww}}}<<<;;;<;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fe5b6a22360f49d0ba27df472e93b550Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_21-47_17233130Seq18094137932_K1322_K1318_testjmadera96-WellK1322_G4_Prunus_psbafKB_3730_POP7_BDTv3.mobSeqPop7z~|zwurqqqpolhd_\YWTPKFB?>?@AA?=<<>AEHJLNPTW[_chnw %A`~z[>"|l^SI@952112344568;=@CFHIJJIIJLPTY^elu"N<bvHwH"Iy4~ v\YeI]E7226<@BBA@AGSfe4qb  :{YB6126=EMU[agnyIS XG1v&|\H?<?DJQ[gxCUq9csN2         $+5ANZcijhdcivj u_8rF;bbP~s@    #(,-,*('*3EcZ CnA#    "/:CE@5' <zoML&F4kc0   !"!#>mQ}-U+      ""1dV_ r> )/0,)+<cd+gxZ={<  #*3<BC?5*&7hL[E]@9?IS]emu{~~&z pviD2,**)'%$$$$#!!'-4893(%M{oU^_4-h?f07jy2"/x:         )4@ILD12`UbU26<1D.     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T  AxA"cARoA"KB.bcpKB_3730_POP7_BDTv3.mobtermAAATTCATCTGATCTAGCTGCTGTTGAGTTCCATCTATAAATGGATAAGACTTTGGTCTTAGTATATACGAGTTCTTGAAAGTAAAGGAGCAATAATAAATTTCTTGTTATATCAAGAGGTTTGGTATTGCTCCTTTACTATACTATTTAGTATTTTTTTTATTTACTATTTTTTTTTATTTACTACTTAACTTTACTATACTATATTACTATATTGTTCTATATTTTTTTTAGTTAATTTACTACAAAATTTAATTAAAATATTAAAAGTTTCAGTTTATCTTGTATTTTATCTTACAAGTAATGATAAATGGTGTAAATATTTGTAATAGTACAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACAAATAAAAAAAAGAAAATTCATCTGATCTAGCTGCTGTTGAGTTCCATCTATAAATGGATAAGACTTTGGTCTTAGTATATACGAGTTCTTGAAAGTAAAGGAGCAATAATAAATTTCTTGTTATATCAAGAGGTTTGGTATTGCTCCTTTACTATACTATTTAGTATTTTTTTTATTTACTATTTTTTTTTATTTACTACTTAACTTTACTATACTATATTACTATATTGTTCTATATTTTTTTTAGTTAATTTACTACAAAATTTAATTAAAATATTAAAAGTTTCAGTTTATCTTGTATTTTATCTTACAAGTAATGATAAATGGTGTAAATATTTGTAATAGTACAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACAAATAAAAAAAAGA &,>N[`t~+8DXgs *8@LWes| #-8CO[iv *7DQ\gr} '3?JT_kw ,8DQ]iv'4ALWcp| #/<HT`my    + 7 D O Z g r ~   % 1 > K W c o |  # / : E Q ] j u    & 1 = I U ` l w    + 6 A M Z g s   #/<GT_kx+7CP]it)5CMYdp| "/:FR^jw(2=HR]hr} &,>N[`t~+8DXgs *8@LWes| #-8CO[iv *7DQ\gr} '3?JT_kw ,8DQ]iv'4ALWcp| #/<HT`my    + 7 D O Z g r ~   % 1 > K W c o |  # / : E Q ] j u    & 1 = I U ` l w    + 6 A M Z g s   #/<GT_kx+7CP]it)5CMYdp| "/:FR^jw(2=HR]hr}  "" (:/$ 1:'4..44:1>64)>>>1#>>>6>>/88>>>6> "&#--22>>>>>.;>>>>>>>>>6>>>>>>>;;>;>)+"%1>>;>8>66>;>>>*11>>;>;;;>>>;>>>;>>>>>>>>>>>;>>>;>>;;>>>>>611>>>>;>>+>>1>;888>;>>.>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8.)8>>>>>88>>>>>>>>>>>>>>>>>>>>>>>$.>>>>&*::::::6::::6*66:/::66:::4%-::6::66::     "" (:/$ 1:'4..44:1>64)>>>1#>>>6>>/88>>>6> "&#--22>>>>>.;>>>>>>>>>6>>>>>>>;;>;>)+"%1>>;>8>66>;>>>*11>>;>;;;>>>;>>>;>>>>>>>>>>>;>>>;>>;;>>>>>611>>>>;>>+>>1>;888>;>>.>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8.)8>>>>>88>>>>>>>>>>>>>>>>>>>>>>>$.>>>>&*::::::6::::6*66:/::66:::4%-::6::66::   /W1 S]D $DI`-A@Y *J=0HY 89FUbBCM[,1Q 2-=K_R8!K F#&#.N F"XG=L(5UV;&' F C7*[ _?aO0+$B-3aB1L'HJ".3'\#32_M'G(NA '>S.^$LcC(U8P\ -IB74*@-<+<)2=2DcQGP%WRK LEJ >H?0cM.2Z)G&1[8O!YJ.'$'\-=a/D-?;, ^6 ^O1a-CJT`H4DWT6PR 6@!dFWMdXWE=8<Wa60:8T?<8\D^B80B >N[J3,^N8&WTP[ A[<27$^VT%9]PF/a<<2 M(B518;+=_ MTaad.j1*|0q} 0Lb2OQ#B=~l*j}ia8P]@+BT"7hqDS(T8~;:3k;0/K4nB]{Ld3]zQU7y'VJ<FJc[> fCu^&T7K]bH(`\G|gVB^{c0y,|_o'E(9j$KJR=ab(.E}rcm*$I U7Zuu.$Gn 965T|v?5)Z# )L HP(xn HjK]irfne)}qct]~gjfU?;9LEVZIAIM?LAE:34>=<)/rJ3!lfB3%-.)$ &(-  ! >      x              %   ' #% !+-2!-% %(#$$#0EppMpppFlsp7u&|YpB!P'#1{a.oPKTs2[k9p\!{|ETt7Q{jrS5=iMruF1tRd<HS}jGkLype'x,0R4Dcdb(V8'd|FuS)dLy% 9%,G<'hdpyRYp;KICF$`KD]gffH(heWB^SZL,X>*$5F~4*@Q;:+ZP%:OIO'"6 X157@ ,))-" 9 <(e'_2>+]/Co40@>-t8!4=>(0:H-65,%> - )84  $ 310Y1~:,JC(2!'.;8 M63" 2F* ?6G !&",* !63;.,(:0?!%4!;!9:"$)+$A\9"[T9/)* '#-&-=>) -"'<Ft#*&-:!)+/2mJ.$H(_)/&Sj@%S*FTTTACTCCTAATATATATGCGGTGGTGAGCTACCTATATTTACATATGACACCGCTCTCATTATATACGACCCCTAAAGGGAGTGGAAGCACTATTATTTCCCTCGTAATATCACCCGAAAGGATATGGCTCTTCAACAATACTATAAAGTATAACCCCAAGAAACTATAACCCCCCATAGACTACTCACCTCCACAAGACTATATAACTATATGGTGCTATATAACCGGGAGTGAGTCCACTCCGCGGTAAATTCACGGTATCACGGGTGGCAGTCCATCTCGCATGAAATCTAACACGTAGTGAGAGGGGCTGTAGCTATAAGTATTAGAACACGCACCGCATATAGCTTCGTGCATAACGAAGGTGCATAGTGCCTCTACACCCAGCGCCGGGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-10 23:17:46 -05:00AEPt3AEPt3APFN APXV2APrN |APrV1APrX}}…ARTNASPFASPt$ASPt$B1Pt#B1Pt#BCTSMBufT"DNCMNT ECTID CTNM&CTOw>CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA )<RxDATA )<Rx7@DATA )<RxDATA )<Rx0DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LmLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS.OfSc jP1AM>oP1RL;SP1WDAP2AMGP2BAJP2RLDPBAS0qPBAS.PCON9PCON87PDMFPDMF.PLOC5PLOC1PSZE`PTYPFPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%.SCAN3SMED SMLtSMPLSPACA^SPAC.SPACA^SVER4.0SVER MSVER,,Satd ^Scal@Scan3TUBEB4Tmpr<UserphARphCH.phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN |APrV1APrX}}…ARTNASPFASPt$ASPt$B1Pt#B1Pt#BCTSMBufT"DNCMNT ECTID CTNM&CTOw>CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA )<RxDATA )<Rx7@DATA )<RxDATA )<Rx0DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab\QFVoc\@FWO_GATCFeat//\GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LmLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS.OfSc jP1AM>oP1RL;SP1WDAP2AMGP2BAJP2RLDPBAS0qPBAS.PCON9PCON87PDMFPDMF.PLOC5PLOC1PSZE`PTYPFPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%.SCAN3SMED SMLtSMPLSPACA^SPAC.SPACA^SVER4.0SVER MSVER,,Satd ^Scal@Scan3TUBEB4Tmpr<UserphARphCH.phDYbigphQLcphTRphTRqk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4{,M09b!#}tl+eFKD.Edo!:Z`+a6:!.tkSY4E2rfJX|PzMWhceS-bb3SvrsPo"`dbp~lV}sT!lZbCd]E>]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|T4}J~B>v09eWf/E0h#X+,Rn-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9Ui|+Zm^NgI]S4S\dX ?Gg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9UZ#0Ym$u;{&;lOA2|Yt34^.??US]Q#V>Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&03BSmv^Y6UziP-#=c$DfqcdsTF]luKgk_lpulAEPt3AEPt3APFN APXV2APrN |APrV1APrX}}…ARTNASPFASPt$ASPt$AUDTzzl$B1Pt#B1Pt#BCTSMBufT"DNCMNT ECTID CTNM&CTOw>CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA )<RxDATA )<Rx7@DATA )<RxDATA )<Rx0DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab\QFVoc\@FWO_GATCFeat//\GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LmLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS.OfSc jP1AM>oP1RL;SP1WDAP2AMGP2BAJP2RLDPBAS0qPBAS.PCON9PCON87PDMFPDMF.PLOC5PLOC1PSZE`PTYPFPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%.SCAN3SMED SMLtSMPLSPACA^SPAC.SPACA^SVER4.0SVER MSVER,,Satd ^Scal@Scan3TUBEB4Tmpr<UserphARphCH.phDYbigphQLcphTRphTR