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'# # !'' !71& -   !     !#                                 )     "                                 )(224&0 4##@-+-',)(815;'?-!6><.0% :',>!$'&                           {",,III^^^{{{&&&999DDDXXXaakvv      **333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRSTTMMMlmm     **)333==<FFEONNWWZ_`fhgooowww}}}<<<;;;<;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 164c0a56f75541e9a507b998d6e60efaSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_21-47_17233130Seq18094137932_K1322_K1318_testjmadera96-Well&K1322_G10_Selaginella_pallescens_psbafKB_3730_POP7_BDTv3.mobSeqPop7zCDGKPUYZ[\_cimoppqsx}|wsokihhhjkkigc`^]]]]\XTOLIIIJKKIFCA@ABDFGHHIKOSX^ciqz %B_{rS2uj_UKC><<>@CEFGHILPU[clu9k"kb6fiBwleba^VC%JLzqkgedefgghjmqu{=tP4GH69,hVKECDEGJMPU[dn{VACHrZJ@;;@GOYdp<s_'`A+    "$&),17>FNUZ[YUQR]{w4H9wCPE,_sd48U*     0Qf;b4 %-6?FIG@618Tj(vb'R $*05995-%$4]#GI1U   $&&#)H} | Fh9  $,6AJNMD7,+@tIvY{]n ,`S#4."ER&  :mr $j1 2U[Qu? !'++("!/M}>n^)sJ/!!&+05:>CGKORTTSQPTb}.vNp[QNNOONLIGDBA?><962/-+)'%#   #%" *ZUnlxHlL. 9s=,Tk@  4V`I^a`/ :dOmoSP" *K|+NVBY+     'N^yBg3  $/9@A<2+0JW[wn_" r e3    /XFbaA T//QFp}i7f3 $&$ (\4G&oU& 4X"85sB   !*251% = 5GO=_X{1aRe# xJ(    .]@|5H)3bY[ sXl umX$   5g;nwJr: (.+  Nbg]] |L)  &)(" FqGwl(8c43W&)zJ'     5UyjG)  *P.#gYnQrj/   #AhsL,   &Iz'\Tv  #k0   An S*    (Q\4   #K~  Q* %Fo\6    &GsYER'}9D    4ZzP.    7^rOCOt)( uWRfW4  /MqtP3     2T{lF& 6s^Db7(4S{a=!   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(#% *&') #4/ 0C- &/;90!5+'"'')22 #'54:$$$$$$D@G8 5,p<MdMpdOYWp,/x|%%:p8dp+ 68d7/d}k8oJp2zd md88o8y8l6!78 PU9d 4E",@Z/,!P8dpDdup'_,w8d @6|07Hj"#=eTQSQK[P9#]xG\|^SL\N#O].9(.?Ie %(`SKni{\5++4>76;SDX3N:-E*;qC#&n=Bt]HcG:3"&;> 6;4"CF:X 8#".",: 3+0-RF0&*GX"KKY+/*!0 +%6+!/,BPlP$5'?@&58:&1r,+#"@ 5%/9"3 3!,g1Q+)B$+=$DFE3#5,%'7</&:1+ ,'(%( &%3 )_, -4)(QY)#(8G{()%'(6iD$( (53(H#)>T25NcLPJhH%ECACCTCTAATATGTCGCCTCGGTGAGCTTCCTCTATTTGCCGATCAATCACGATACGGATGATTCGCCCCCCTAAAAAACCGCTGCACCAGACACCATAACACGATACTCCCCCAGAAACTCTTAGCTGCGACAGTAACATTACTATATCGCATCGTGTTATTGCACATCAGAGGAAGGAGGAACAACCATCCTTCGTCTTACTTCTACTAGGAACATAATACTTTCTCCTTGGGCCACGAGGATACTTAGTATTAGTATAGTACACCGCTTACACAAAGACCCGCCTCACATCCCTACGATCATAATGACTGAGTACCCTAACCAAACAAACAAAGAGAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-10 23:18:00 -05:00AEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtCASPtCB1PtBB1PtBBCTSLBufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SDATA )S7DATA )SDATA )SDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~J~LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS0P1AMV>xP1RLV;P1WDVA$P2AMVF|P2BAVVI(P2RLVCPBASVV2rPBASVV1PCONVV:vPCONVV9 PDMFPDMF1PLOCV6tPLOCV3PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%0SCAN3SMED SMLtSMPL''zSPACMSPAC0SPACMSVER4.0SVER KSVER,,nScal@Scan3TUBEB10Tmpr<UserphARphCH1phDYbigphQLcphTRphTRKThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtCASPtCB1PtBB1PtBBCTSLBufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SDATA )S7DATA )SDATA )SDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabZbFVocZQFWO_GATCFeat//Z"GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~J~LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS0P1AMV>xP1RLV;P1WDVA$P2AMVF|P2BAVVI(P2RLVCPBASVV2rPBASVV1PCONVV:vPCONVV9 PDMFPDMF1PLOCV6tPLOCV3PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%0SCAN3SMED SMLtSMPL''zSPACMSPAC0SPACMSVER4.0SVER KSVER,,nScal@Scan3TUBEB10Tmpr<UserphARphCH1phDYbigphQLcphTRphTRP'd}|(Hfz@jXa*`#|nh-u?[-0#Osp_0_9v8ZIYW<|eN$&i[D:0M,m(o~%Md02R P,hzp!tY`-'Icf]h_m7^d`7Y{v]qoUYoOyl}$r&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hDM=VrTH&zqru+!hs|O1i~?z;{W8<)mbo)6s6IxO\JpSbwEiv5&o58J.:)'C#x5ots/H1C:2#%=c/a}ayHbc\S.Tqt6IEUYC~n{[._LbR/"E.Om,NeLVWY+a:WkeC.%oh^5Q1V?|&@8je]i~mcA=@9WA^ re/ Co8Z5v4*un,T(k"T'ogLc{Xm|3HF)SE8n\qo(t&&"V1a |+Lw.qsA],?y$.d76pJJmQ;R#hfMi\bT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m*0_fs$X=<;#(eaK|`!t/[z~+7D>0Mt9!'gURmuulowDC{R7R>(hZ$~I!|spN$t.Dd^4G^({C!RJ<6Q^Zew.k.d}uNf1&vM\#YTEl?&azzHFH9bYz.R6 4z[qNyOA58rm&w_bU=^R:^y\1|(D$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUGxP1RLV;P1WDVA$P2AMVF|P2BAVVI(P2RLVCPBASVV2rPBASVV1PCONVV:vPCONVV9 PDMFPDMF1PLOCV6tPLOCV3PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%0SCAN3SMED SMLtSMPL''zSPACMSPAC0SPACMSVER4.0SVER KSVER,,nScal@Scan3TUBEB10Tmpr<UserphARphCH1phDYbigphQLcphTRphTR