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'     "                 &                                                                                                                                                                                                                 "                          KABCDABCD{",,III^^^{{{&&&999DDDXXXaakvv      **333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRSTTMMMlmm     **)333==<FFEONNWWZ_`fhgooowww}}}<<<;;;<;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c47afaa6a441430092373fb2ddcba140Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_21-47_17233130Seq18094137932_K1322_K1318_testjmadera96-WellK1322_F3_Urera_verrucosa_psbafKB_3730_POP7_BDTv3.mobSeqPop7z !""""""""#$%%$#! 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LPV`n~|si_TI<,tle_[WUTUX]dlu;a1] 2Uqq]E'mS4oFn]PE=730/...036:?DKUct3XqHlVD7.'" !!"#&),048=DLXg{ Lyd4oYH=7458<CKWg{^L+XnkO+r rQ:+    !#%(.:Lh='7vL/ !$&(('$ !-GqM$A>>c8 "$$#!"+?dELw|[Y[,   "!%K@>S<I ] <Dt g5.T.nv>         "&+2;EQ^m~#Gj.o.t_4jD%bA!o_QE:1)"        9`"E1}G  PY4<   #'& ^)W;     "UFu0cE<DTetY0H       &Y+XcJCOD_gLfw|c(   5udV e+        +a-NL']*     ([%JN/h2   !"$&')+.13555420/.--,,-/2452+ ,V+4t?     6k!s>    I(zE   3e  wH3<_d:    =j|O*   3d+*g8    &Di|W7%$7W~^:  +Fdx\>$ +E_uxcJ0  6ezM>S2/I&%++&#1E\rudVLIO^rhP;+! "$&),/00/-*((,7Ha~vX@/%!"%''%!"2Nu eE.!  !*E@)A(ARg8AKB.bcpKB_3730_POP7_BDTv3.mobtermACATCTCTGACTAGCTGCTGTAGAGTCTTATCTACAAATGGATAATACCGCTATATTAGATTAGTGTATACGAGTTTTTGAAAAATAAAGGAGCAATACTTACGTTTTTCTGTCAAGAATGTAAGTATTGCTCCTTTTCGTTAATAATAATAATTTGTTTATGTTACAAAATAAAAAAAAAATTCTTATTAACGTTGTATTGTAAATTGAAGTATTAATTACTATAAATTAGAGTCTATTTTCCTAATACTGTTATATAGTATAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATAACAAAAAAGAACATCTCTGACTAGCTGCTGTAGAGTCTTATCTACAAATGGATAATACCGCTATATTAGATTAGTGTATACGAGTTTTTGAAAAATAAAGGAGCAATACTTACGTTTTTCTGTCAAGAATGTAAGTATTGCTCCTTTTCGTTAATAATAATAATTTGTTTATGTTACAAAATAAAAAAAAAATTCTTATTAACGTTGTATTGTAAATTGAAGTATTAATTACTATAAATTAGAGTCTATTTTCCTAATACTGTTATATAGTATAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATAACAAAAAAGA 0I[`r~)4FWamw$0;DQ\hw(6@KT`kx#.:GT`ju $/;HS^gr}+7CO\ht)5BOZfs}*6AMXcoy  ! - 8 C P \ h s    $ 1 < H U ` l w    , 8 D P \ g s     & 1 = J W d q |    ( 3 @ N X e p | *6BMXcnz 0I[`r~)4FWamw$0;DQ\hw(6@KT`kx#.:GT`ju $/;HS^gr}+7CO\ht)5BOZfs}*6AMXcoy  ! - 8 C P \ h s    $ 1 < H U ` l w    , 8 D P \ g s     & 1 = J W d q |    ( 3 @ N X e p | *6BMXcnz '#5   %5#*.4! &(.+>*>6>/>688>/61> '>1/// '3"" ""!83>>.1>6&1>>>>>>>>;+1+&3>>>"';>6.;88>>>>;>;>>>>>>61>>>;>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>66>>>>>;>11>>;;1.8;>>>;>>>>;>>>>>>>>>>>;>>>>>>>>>>>>>>>>;>>>>>>>>>>>>8>>8>8>8&*&>>>>>>>>>>>>>>/6>>>>>>8>8>>>/6>>>>>.8>>>>06>>8>&/>>>   '#5   %5#*.4! &(.+>*>6>/>688>/61> '>1/// '3"" ""!83>>.1>6&1>>>>>>>>;+1+&3>>>"';>6.;88>>>>;>;>>>>>>61>>>;>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>66>>>>>;>11>>;;1.8;>>>;>>>>;>>>>>>>>>>>;>>>>>>>>>>>>>>>>;>>>>>>>>>>>>8>>8>8>8&*&>>>>>>>>>>>>>>/6>>>>>>8>8>>>/6>>>>>.8>>>>06>>8>&/>>>  NIZE,M3;8  RC3V#NJ:>_&Ac\UDF0!%5^*E2c &==_^54:8D]W=./  %F6F@[. d) O6K+- 04(= T<H<Z) EK 'a$Qa \/;6;?X+%I()I.8QN :_93Y)D7>Q$Z9? )V2EU,^?=>6CPQ$F\c!,U)$GD@[,b B2]" 6VX1ZXKL9I`-M'$LJ,$F\DNA%>bA:M5#-D"I3B]a. 1$-TOXJ/WECE%QYRRHa@J '^A6UJ%T <TU] % 4\Jh<ldGWl:(}("^w-&zHiT?=H{oWza53^tkjWW 6ku8^1X<p.@vzEhV0[LcVNacE&O,0 ^UaB`;s+^A B0[O:PT\<6+RGPO @96Dko##w3V[>9|={K;I\,"T!S a HC~ HRXl ! nlev[@Q^ScUGO93@GMR=?)!135+3!'+4*$(,%  - P        34,                       !!!dWppqeTp`c NmKN oD%C?ntP> hD6sp(4,,^YRd 6";y=0~u^D,L M }8m1p%vsx6 )'O1Q0O#Rm{mN8aFd W,7e OR8`^z?X 7_{VS J~ERS  T&-C.4FWk=& !\+ 53?nqO4,A Gr/5'7'&49-[Li36$.Wv<@R8726)=N7<"7.%28 & $='$34.   1H34>?z1<^'*E* +/!)+8+56>,J(-./ ;:*5!<,G"7T#eH5Z&( ')<(#A'1 X0#M2Y=0(6+S?8765N #@ISCAI91A}NWY7m/CATATCTAACAATATGCGGTATAGTGTCATATACACGTACTTATTACAGCTATATAAGTTAAGTGTGTCCTCTTAAAAGAGGGTTATGGCAGCATTACTGGGGTGGCCCTCCCTCGAGTGAAGGTATAGCTCTTACCCGTGATTATTACTACTAAGTGCATGTCCCACCTTACCGGGGGGGTACTCATCCCCGTGGTATCGTAGGTGGAGGTATAATTAACTATACGTAAGAGTCTATACCCTTACTACTGAAATATAGAATAGAACCGCATGTAGCTACGTGCATAAGGACAGTGTATAGTGAGTCTACTACAGGAGCCGGGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-10 23:17:47 -05:00AEPt3AEPt3APFN APXV2APrN „APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT"DVCMNT MCTID(CTNM.CTOwFCTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D~DATA"DDATA"DDATA"DJDATA 2ddDATA 2ddIDATA 2ddiDATA 2dd1DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~SLIMS!!ZLNTD2LsrPaMCHNMODF {MODL3730NAVGNLNE`NOISxOfScnP1AMEP1RLE\P1WDEpP2AMEP2BAEEP2RLEPBASEEyyPBASEEx4PCONEEPCONEEPDMFPDMFxPLOCE}HPLOCEzPSZE`PTYPNPXLBRGNm RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%wSCAN3SMED SMLtSMPLSPACMSPACxSPACMSVER4.0SVER SVER,,Satd^Scal@Scan3TUBEA3Tmpr<UserphARphCHx/phDYbigphQLcphTRphTR:This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN „APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT"DVCMNT MCTID(CTNM.CTOwFCTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D~DATA"DDATA"DDATA"DJDATA 2ddDATA 2ddIDATA 2ddiDATA 2dd1DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab7FVoc&FWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~SLIMS!!ZLNTD2LsrPaMCHNMODF {MODL3730NAVGNLNE`NOISxOfScnP1AMEP1RLE\P1WDEpP2AMEP2BAEEP2RLEPBASEEyyPBASEEx4PCONEEPCONEEPDMFPDMFxPLOCE}HPLOCEzPSZE`PTYPNPXLBRGNm RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%wSCAN3SMED SMLtSMPLSPACMSPACxSPACMSVER4.0SVER SVER,,Satd^Scal@Scan3TUBEA3Tmpr<UserphARphCHx/phDYbigphQLcphTRphTRXPvc.[.u2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*P7C4%r3$LRu~_`:\H|#{mbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@E4Kw%V,ep> 9*#pPonwQ\#:TT!&FBcn#N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#ky6Ka;v8e7s]jTj.uFn4o[|.]/;7hgCp&NU826D31:,M n921kMYYH|O[nY?KPQh^k 1{sI(J8dM!tqw8&rd+'n,J6v$NU,l9cGY@ZWeo)GjL~7L3"6%4y^.N82`\wmj7-U.*P-*KWeq }X'K2MWhc'kT'_C:rF(5,(?z7b[z`>F3!sY#+~/]m#NA^6h9e *E&]E64Ut.K5Z ~/$SfMm;c4|tkt7g& xvU+}\UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4