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&(        0{",,III^^^{{{&&&999DDDXXXaakvv      **333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRSTTMMMlmm     **)333==<FFEONNWWZ_`fhgooowww}}}<<<;;;<;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0186ecf8f4df4f278c7819856cc38b5aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_21-47_17233130Seq18094137932_K1322_K1318_testjmadera96-WellK1322_F1_Trema_micrantha_psbafKB_3730_POP7_BDTv3.mobSeqPop7z!""##"!!!#&(*)'%#""$%''&$"    "*2;EOXco~ %A\uu[?!}iYKB:50+&!!$(+-.//149@JWfx;[z}eG%yfZRLGC?>@ENXcn{RMv2?e|Xm`YVUVX]gw NhU3y*,Z pVF>=?EKR\j}S $X lUE<8678;=?CITe~9x-XrzmMEV7#  "*17<@GSjop >j7    !#%).4;CJQVZZYVWa}R)*[}4,])u>       $%$ $=ii)gn5wi6  %)*' %J*,U-   "*3:;6)I);D}2    "+5@KSVRD0&dZ:2Vq2&4AJQW\_abdehkosvxyxwutsstvxyyxtplhfddcb`\WRLGC@><;:962.*'&%%%$!!%)++)(),021*2k=@wF    #%')-27:6,!M.4JD!-/K%PD1Sh[8l#        $'$ V?M =  #/=HLF22pubjpcSjpqhw0    #-8CMPH46\C9\k #%%#         #,484&"~DX15      $(& W8,nt<Sm)           1xEzV   ,8=8(+m7(2l! 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FWA7aHM0;, D`.D,B!S H.=G'!T1 :CZbI#D%G5Dd 1+C17&$aHU@:QQPCD42 A*0+F/MA@5`(, ; V_)#=P>Q*EON$T`>+1!\-IQ[/G!R<Q@#U&E / 1cCRQ^^?LH#R2/MHDMV-39'9+C-b/V8KW:&2BTZ)37-X$:9:-# #*.]YMRX SJ# AJB4S-;G Z IPU>!8-P GJ6-M>1I#C)DEM,V"F;?!1; YLHX<^UN>;3\>22 1>#'-';-2KAWC3? : ) G. 8YZ1IMTU#7"KM?M5"MFa,cMEG38P+$+29@FJ25M&MMMYOF7J 2M 22K!MYJ\J/22.^A#MQ*QV-bN c `1j`F^ o&M f M{I"=VA)8A^DLi~h)mJEoE7RuGlS%'1_|%OH][~d 9x#9!R{r1Rk" c7;WxE~Zneg#a)UawgU^#kLq(KBv/ J;ldQ& :/Uwtl}kB%Rrb]KSLS)WE'{%y[v=Q|<!)#"h0{bx /n  O\]D(W3d@=4 r<ql(E#D NY[}gCQy  Wj4'nWVxm-nT*v>r#X0zXWy!L HPf7<^eY<07#xvaXHbgED6EH2NV=9C,327=89&3042]G76                  xxxxxxxxxxxx         !    #"( &$ 1&-*!  )CCCCCCCCC@CCCCe|R:CBWCCCCCCCCCCCCCCCCCCCCCoC CCCCCCCCCCCCCCCCCCCCCCC@ CCCCCCCCCCCCSbC 7Bfjpp  Lx?p6_@Vdnsdy+u^9UD'yFO(BdlqkFP-)8g8: 8c=:%z?pYbdOlSp[ CaW\7ckV`'^YL=d:m+S8&/2^868+RC8p,OdpdPHi,8#^/`Y$ppzG!d=d51du~N 1A$*1 {=`kiQLRHikvQ Z.l.C'Q91F,*j<"##21+33ms4_joj1 0>` :,&$$ (*#27UECL9'72 # ,8'7B 7,"'6  D 8(A#%0+ %=899@/H=0;,<&+703  /={6 6">"S C,%{24AB4&FTr+,,N1D 8<2)+(.4:48CH#,(c&;9#-b<(->!u <7' T.$M0B<=(" J;&4*+"0 "72G4S[6O5/_/ |i+#,wYW*kMPv?Lme 5UtmW-0 4s +[)=rF4U=>:ATCTCTAACAATATGCGGTAGAGTGCTCTGTACACGTGAATATGCCACCACTATAAGTCAATATGACCCCCCAAGGGGAGGGATAGGGACCCACTATTATTCTCCGTGCCCCCTAGAACGTCAATCTCAATAACTAATAATAGGGAAGTAGGTAAAAACCTCCCTTCCACTATATACATACTCACGTCCCGAAGAAGTAAAAAAAAATGTGCCCCTCACTCTCGCCCCAAAATGTGCGGAATACAAGAGGAGGGTATTATTGACGACTGTGATATTTACCTATTTAGCACTATCTATGTCGAGGTATATAGAGGTATACTACGTACTATCAGAATAGCCCCGCATGTAGCTACGTGACTCACGAAGGTGCATAGCGATTAAACACACAGCCGAGGGAGATGTCCTTACCGAGCCTAATGGTAATTTTTTTTTTTGTTAGTGGGAACGTTGCCGTTATCGATCAGAAATGTGCTGGTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-10 23:17:40 -05:00AEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtIASPtIB1PtHB1PtHBCTSQBufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6DATA (QDATA (QڛDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~P3LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS,P1AM?nP1RL;P1WDC(P2AMJP2BANVP2RLFPBAS.PBAS,PCON9PCON7PDMFPDMF,PLOC4@PLOC0PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%,SCAN3SMED SMLtSMPLzSPACAaSPAC,SPACAaSVER4.0SVER QSVER,,nScal@Scan3TUBEA1Tmpr<UserphARphCH,phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtIASPtIB1PtHB1PtHBCTSQBufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6DATA (QDATA (QڛDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab`FVoc`FWO_GATCFeat//_GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~P3LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS,P1AM?nP1RL;P1WDC(P2AMJP2BANVP2RLFPBAS.PBAS,PCON9PCON7PDMFPDMF,PLOC4@PLOC0PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%,SCAN3SMED SMLtSMPLzSPACAaSPAC,SPACAaSVER4.0SVER QSVER,,nScal@Scan3TUBEA1Tmpr<UserphARphCH,phDYbigphQLcphTRphTRYxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["r87xFMWx4Zh+gluc|fgtg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLV`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C]8p39VabgHf99^q+Is:p:H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L