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(   i        {",,III^^^{{{&&&999DDDXXXaakvv      **333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRSTTMMMlmm     **)333==<FFEONNWWZ_`fhgooowww}}}<<<;;;<;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8d6d79ff58404dcba4300572d73b573bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_21-47_17233130Seq18094137932_K1322_K1318_testjmadera96-Well"K1318_G2_Lycopodiella_cernua_psbafKB_3730_POP7_BDTv3.mobSeqPop7z !! !"##$$$####$%&'(('&$$$%&''&%$$#$$$$$#! #,5?IS^m~DsYJ 0M`ih]K1tO-lL+ xP(o[L@830.,+)(()+-.//.,++,.159=AGNXdt2QnyW2 hR@1&!&,5=GPZfvc3#F|k%J;KwU?2,+.39@GNWapHJ$FM8 rQ{T>3148=BKYp!X ^ }W:%     $+28<AJ^4$k:9j2 "$&(-4=GPVWVTVe*M,ri3;JKtb1         %-3651+&);coJX d4  $'&!5aY$#E*u_$yv0      %))$%Rk&OHG}: !"#$%'*/49>ACCCBBCDGJMOPQRTX^elqttromlllmmlkjigfc`]ZYYYYXTNG?94210.*%     %)*&:qxn \>Uu|*0\!     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"(.38<AHTh91t{<Hr`\ey USE|qyN5)')-258;?CHLOONKJPab5]tB%    5[*zqO(  &,0/*!!H+0kd#X@e( \, %-5<AC>4%)Z*T6W7Y1\# "+6AJNJ?.)\9rVrHRH     #).49?FP]n$FlEv 6[{uM!_-qG!xfVH<2+'$$$$#!    ! #')(" =P 1#[$           %.0+ +yu`{*%K     $+4>IS\emv}sh^TJB:3.)&#    Vbw m/     ?`mI    >1yn"w7    '_Ok*C          'h-tX   6w!i_o2  ?3zci,    "YExg(E   !$&''(())****))('&$#""""!    G)avc.P    /dDt~`!dhQLfe#_zl<b0 <t6TO'`?Etc|;R#   A9<I E5G7 L$  ,e@ec:\33[:ehFt;   >s(O'     "N$*f4 "Q5;!o;  6l)~G     7m$t@     (SO)    4^e?0;_]4     3W|`=!   !P2,zSW/l|[k3(+&-Ebl_^ioN6("!""! "#$%&&$"&9W~kK5+(*-.,& !2S:bqe@ eC.$!!! "*18=?<70'   " A p;A,+AZ.IA KB.bcpKB_3730_POP7_BDTv3.mobtermACAATCTCTGCATAGCTTCTGTTGAGCTCCTTCTGTAAATGGCTAAAATCCTTCTTCAAGAATAGGTTTTTAGTTATTTATTATCAATAGGAATAAATAATGACTATATTTTAGTAGATTCATATTGATGAAGCTGGATCATGACTGGAATATGATAATAATCTTTCAATTATACAATGATCTTGGAGTAGAGACTTGTCCCGCCGAGTCTCACCAAGGTCATTATTTTCATTGTAACATATACGAGAGGGTTTTAAACAAATGATGGCGGATGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCATGAAACAAAGAACAATCTCTGCATAGCTTCTGTTGAGCTCCTTCTGTAAATGGCTAAAATCCTTCTTCAAGAATAGGTTTTTAGTTATTTATTATCAATAGGAATAAATAATGACTATATTTTAGTAGATTCATATTGATGAAGCTGGATCATGACTGGAATATGATAATAATCTTTCAATTATACAATGATCTTGGAGTAGAGACTTGTCCCGCCGAGTCTCACCAAGGTCATTATTTTCATTGTAACATATACGAGAGGGTTTTAAACAAATGATGGCGGATGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCATGAAACAAAGA 2B\btz (4BRalw*6ALVbkx(6@MYeq|)4@M[hr|(5BNYeq} !-8DO\hu%/=IT_kv&1<GT`mx  , 8 D Q \ h t   $ 0 < I T _ j w    % 1 = I T ` l y    ) 5 @ L Y d q ~   $ 1 < I V a m x  !-:EP[fp} 2B\btz (4BRalw*6ALVbkx(6@MYeq|)4@M[hr|(5BNYeq} !-8DO\hu%/=IT_kv&1<GT`mx  , 8 D Q \ h t   $ 0 < I T _ j w    % 1 = I T ` l y    ) 5 @ L Y d q ~   $ 1 < I V a m x  !-:EP[fp}! %) 2%+*/-3):6* +":)*4>>>/&>>>.>>>/>> >>/>>  ,-' (;;;;;>>>>>;11>>>>>>;>;;>>>>>>+"");;;>;;>>>>>;;>>>>>>>>;;>>>>;;>>;;>;>;>;>>>>>>>>>>>;>>>>>>>>>>>>;>>>>>>;;>>8>;;;>>>>>>>>>>;;;;>>>>>>>>>>>>>>>>>>>188>>>3>>>>>>>>>8>>/6>>>>>>>>>>>>>>>6>>>>>>//>>>>>>8>>>>>/6>>>>>>>>>>>//>>4>>,>>>( ! %) 2%+*/-3):6* +":)*4>>>/&>>>.>>>/>> >>/>>  ,-' (;;;;;>>>>>;11>>>>>>;>;;>>>>>>+"");;;>;;>>>>>;;>>>>>>>>;;>>>>;;>>;;>;>;>;>>>>>>>>>>>;>>>>>>>>>>>>;>>>>>>;;>>8>;;;>>>>>>>>>>;;;;>>>>>>>>>>>>>>>>>>>188>>>3>>>>>>>>>8>>/6>>>>>>>>>>>>>>>6>>>>>>//>>>>>>8>>>>>/6>>>>>>>>>>>//>>4>>,>>>( ]-:3HE^LC`=E7E ;=5$=6FM/O6_IMJIEB7>< AG&"3^-G2c?;. U.XD=)%2d^(Kc#6 2 K:9/=: 7]C:Rc,=3@'#H^HC%&B*]]<P/43^A2> ?9-])6ba J09 6B WD1%H>`:IUE<; 0.B? <\A3aI0K;a/ (XK !DVM<)'4L/[ ]']S)E2\WA J(QS)0;6"dSHTbBAc S @%C88*4`?a 4J200TG#TB7Q^)5*Z2]"6 >"I( 2X#'*+XY ^z  IrCi sQvIZ%A7XVQ&=prl&P<O/W!'XQjD}~*[BG{BSJ.bL{yio*iXtjaEY$S*+VJBU#}7|It_C 5;O@ A@rHOi[;#kd5 FLjG?: !AIRQP%EY4a.X Tx/ H"1 [ (n! `!'~wjrugq@TgWN:j)77 76zk>HQ5.0)("T 3                                     5`}pp{ppspp< Up%h0NpanOrd+X0O d87pdbH(: 'K L$B!|pd#C78{jH5}SJ*:v8i{%V7}d  ]&Qp8 4,,p8www UerwFqZ&e @A-Z8\]>:-}0FXy@C2K#*W|[;-+) Fyo Ej^nE.B5Y1% -(-V'O'8.!j0k1c,^JP09"!5.$5$:/-\*:G-2$_-$8-;*&,4/0"+6Q</@>'.G*36DSQ0&@#?,B 26GV6).)/,73-3$B" %!Gn0% %2(,"J8CFA1)4!!I47D'5*.I8L)5&;'# =+"36"H"1"%#FD1B8^5BI(tWhaXrR CACCCTCTGTGCATATCCTCTGGAGATCTTCCTCTGTTTACCGATGTTAATCCTCCACGAGTAGTTGCCCCCCCATAAAGGACCACTAGCATTATGTACTGACTCCATGCCCCCCGATAAATATAGAGGAGGCTGCATAATGACTGCAGTATGATACTACTCTCACACTAATCCACTGATCTAGAAGTCCCCACTAGTCTTGCGGAGTCTCACTAGGATCATAATAACCTTGGTAGCATGGACGAGAGCTTGGAATGCAGGTGATGCCGCAGGAACCTACGTCCATGAGGACTGTGTATAGTGACTCTTCAACAACCGGGCGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-10 23:17:54 -05:00AEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtB1PtB1PtBCTSf5BufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,YDATA ,Y>DATA ,YDATA ,Y{DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~dLIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOISLmP1AMDYHP1RLDVP1WDD[P2AMD`P2BADDchP2RLD^XPBASDDMPBASDDLPCONDDU|PCONDDT8PDMFPDMFLPLOCDQPLOCDO(PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%LeSCAN3SMED SMLtSMPL##zSPACMSPACL}SPACMSVER4.0SVER f*SVER,,nScal@Scan3TUBED7Tmpr<UserphARphCHLphDYbigphQLcphTRphTR ;This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtB1PtB1PtBCTSf5BufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,YDATA ,Y>DATA ,YDATA ,Y{DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabtFVoctFWO_GATCFeat//tPGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~dLIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOISLmP1AMDYHP1RLDVP1WDD[P2AMD`P2BADDchP2RLD^XPBASDDMPBASDDLPCONDDU|PCONDDT8PDMFPDMFLPLOCDQPLOCDO(PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%LeSCAN3SMED SMLtSMPL##zSPACMSPACL}SPACMSVER4.0SVER f*SVER,,nScal@Scan3TUBED7Tmpr<UserphARphCHLphDYbigphQLcphTRphTReZ>\&bD8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|Nq_MU39b04Z*PU0P_fSiVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*G* ~.1ON .Oo66iAW[!/> zPuEJeR%$i`8c_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl+9eh>m]#'uyv\R>UTEf6ml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~fS[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0DATA ,YDATA ,Y{DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabtFVoctFWO_GATCFeat//tPGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~dLIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOISLmP1AMDYHP1RLDVP1WDD[P2AMD`P2BADDchP2RLD^XPBASDDMPBASDDLPCONDDU|PCONDDT8PDMFPDMFLPLOCDQPLOCDO(PSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%LeSCAN3SMED SMLtSMPL##zSPACMSPACL}SPACMSVER4.0SVER f*SVER,,nScal@Scan3TUBED7Tmpr<UserphARphCHLphDYbigphQLcphTRphTR