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**)333==<FFEONNWWZ_`fhgooowww}}}<<<;;;<;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 183c9e9bab084eb19d2aef1f539ef518Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_21-47_17233130Seq18094137932_K1322_K1318_testjmadera96-WellK1318_F1_Ageratina_psbafKB_3730_POP7_BDTv3.mobSeqPop7z[[[[\]_^[UNF@=>AFIIFB?>?CHKLLIGFEFGHHHFDCBCEGHHFC@>>AEKPUXYZZYXWUSQPMKGC>940,($ 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KA=A+APgAOKB.bcpKB_3730_POP7_BDTv3.mobtermTATCTCTGACTTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTGGTCTGATTGTATAGGAGTTTTTGAACTAAAAAAGGAGCAATAGCTTCCCTCTTGTTTTATCAAGAGGGCGTTATTGCTCCTTTTTTTTATTTAGTACTATTTGCATTTAGTACTATTTGCCTTACATAGTTTCTTTAAAAATAACAAGGACTTTTTAAAGTTTGGTTCGATTTGCGTGTTTTTTCTTTGTATTCATTTATATTATAGGTTTCTATTTCTATATCATATCCTTTTCCCAATCTTTTATGAAGTTTTATTTCCAATTCAATTTCAATCGAAAATAGATAAAAATGACAATTTTGCTTATTTATTACTTTTCTTTATTAAATAAGAAAGAAAGAAAATGCTTTTCTTATGTTAATGGAAAAATATAGTAATACTAGATAATAGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCACAATATCTCTGACTTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTGGTCTGATTGTATAGGAGTTTTTGAACTAAAAAAGGAGCAATAGCTTCCCTCTTGTTTTATCAAGAGGGCGTTATTGCTCCTTTTTTTTATTTAGTACTATTTGCATTTAGTACTATTTGCCTTACATAGTTTCTTTAAAAATAACAAGGACTTTTTAAAGTTTGGTTCGATTTGCGTGTTTTTTCTTTGTATTCATTTATATTATAGGTTTCTATTTCTATATCATATCCTTTTCCCAATCTTTTATGAAGTTTTATTTCCAATTCAATTTCAATCGAAAATAGATAAAAATGACAATTTTGCTTATTTATTACTTTTCTTTATTAAATAAGAAAGAAAGAAAATGCTTTTCTTATGTTAATGGAAAAATATAGTAATACTAGATAATAGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCACAA)=Y^r} !/=Qamy /7CO\jt'3=HUdr} "-9DQ^it*6BOZfp{ !.:FS_kw(3?JWdp} $0=IUboz  , 7 B M Y e p {   $ 0 < H T b m y  " - 9 E Q ] i u  # / : F R ` l x    ( 4 ? K X e q ~  $/<HT`kx)6BO[fr}&1=HUblx  ,9DP\hu&3?KVcnz #/:ER^ju)4ALXdq|'4@LXer}&3>JVco| $09DT`mx)=Y^r} !/=Qamy /7CO\jt'3=HUdr} "-9DQ^it*6BOZfp{ !.:FS_kw(3?JWdp} $0=IUboz  , 7 B M Y e p {   $ 0 < H T b m y  " - 9 E Q ] i u  # / : F R ` l x    ( 4 ? K X e q ~  $/<HT`kx)6BO[fr}&1=HUblx  ,9DP\hu&3?KVcnz #/:ER^ju)4ALXdq|'4@LXer}&3>JVco| $09DT`mx  $'( 23.::)5&":-::::6/>>>/886>)'>>>>86>>  ""(+3;;;>>;>>>>>>>>>>6>>8>;>>;;+1.+(18;;8;;>888;;>>>>>>>>;>6.0081.>;;8601883166;;;>;8;;23;;;;;13$22.;8;;22888;;;;;;>>6;>>;;;;;;;;;;>;;;;8;8;;;32;>;;;;;;;3;;;33;;;;;>;;333.2+1+8;2..'33>;1+.++.+1+;;;;8>;>88;;;;;8++'-"->;;;;;;;3;3-+'++;;88;>;88338-883-)3"#+-3-3+&2,&**3882& &%)8333882388833888" ! !222&8883>88>)>/.2888/88+88+'!8$.8.*+*./*&*24*6++2+*461644)46'!!.4%6:" "*!44 #*.5*/. "     $'( 23.::)5&":-::::6/>>>/886>)'>>>>86>>  ""(+3;;;>>;>>>>>>>>>>6>>8>;>>;;+1.+(18;;8;;>888;;>>>>>>>>;>6.0081.>;;8601883166;;;>;8;;23;;;;;13$22.;8;;22888;;;;;;>>6;>>;;;;;;;;;;>;;;;8;8;;;32;>;;;;;;;3;;;33;;;;;>;;333.2+1+8;2..'33>;1+.++.+1+;;;;8>;>88;;;;;8++'-"->;;;;;;;3;3-+'++;;88;>;88338-883-)3"#+-3-3+&2,&**3882& &%)8333882388833888" ! !222&8883>88>)>/.2888/88+88+'!8$.8.*+*./*&*24*6++2+*461644)46'!!.4%6:" "*!44 #*.5*/. "   K=+AL1D @ D#+C+HNRU:I)I\0a:NP#0=.Y/^`d?AWO%-Y%U $PKCR &21GaTC+R d8C/(d#5 *Z3^;9D#O RH4E %9&9"W/"'bI',&HcY:U#bMME;\$%8 b*b/.>(TX ?Z2 54A]W2JR3Z(CM)M8 7JU25HA7QM`T"/B?P%P  TLP*1G -]T%N:>O&ZG Y HMP%9M/="AEbWE8!Y90M',T_2'A@KB \X)>8H\XR_E&B=!b. !dSNM=42&[YD/c7/#]&O"D:aZ+ ._@\Q]_8A5?2T &72B*-)b,ZDU"HX!I=&;)RC>2RV%W(W8= Z+F QN+=VT-_>=JA P/ 7 0? VM\-M-C0>QGK9Y /2R6*R>S LQ&*yK0?;e@dMl;r,Hf NCoD*b `& PvxadG53:+G eM1-~B%";0:Rsx.@3GLVx]0[&)o}s`mvjOz@PTA oWJJ&_J5ZUD{;~cdpUb$PBc@p/o/)0~;\*N!ZYyI_]U%My<Fd+Fu)>qDAVp-{NK&|%8jiaU[Pg,9sJ@V.kx9Cn4-tv[k] n> LYWBit\ acnl?].>[~3 Tr]!wAaiSuvi>CgMjAfhiF  *?A<M,C=';<3#/-7F<-r|#.   m                 .  )   ' ) 1 !)$!& -"2!'6, ) ":%:(@(-)-##&C&C-G'2>0$$!!L0F,2!!$1!@6 ,F9:E!!P$$!$$!3 $!$!!gppppQ,p//b~>`pp }dY=7<pd^l{d]*Wwy>kYddq=iyp%  ipKd(4@0_zk*&?ajp;[o.aEr<|~g,P<mdc6[D/_;B&Mw<Dd19@!Y xBV@l/'HAImlw$jWcd%78} :"AMc6TpBCp[6p8:I`j}<Po09WxG}Q-/+7_"1-16."9=gl+('>6vbZg[>!A5A3M+)(1016&B$AM+>1^<zlhM}U5<==>"fw]C" EA.d:b-ITnx1'OJ7*]T1BYs>aQn(h[_W`wPBy&9 Im+k.P3t. t+#uC~dr9ptYz}Sypp\6xj"rkJsN'+Y{)QBazJ/F*7uEHv<tw6;F~ XsE_Dc[W|ng+ ZH&&|76$)d8?*c\Q2: *=1zgVhm.}m>Cd*'6r "% c_(.mMojjamcHbsUlzhzu+ji$>lCQwW%EUlGJATATCTAACAGATATGTGATAGTGAGCTACATATACCTAATTAGGCCACCCCTCTCCTAGACTAGACCCCCCCAATGAGGTTTCTTTCTTCTATATACAGTCTAGTAAACCCTCCACAAAATGGTCATTCTTACCAAAAATCGATTGCAATAGAATTGGAGAACAATCCGCATAACAGATTGGGAAAACCCCCAGGAGTAAGAACAGATGCCAAGAAAATATAGGAAACCAAGAAAAAACACGCAATACGGACCAGACTTGAAAAGGCCTATGTAATATCTAAGAAAATTTGGAAGGGAAGGAGGACTAAATGCCAATTGGACTTTATAGGGTAGAGGGGCGTGAGGGCCCCCTTGATAAACCAGGAGGGAACCTAGGGCCCCTTTTTTGGTTCGTAATCCCCACACCACCCGACCGGGGTCTCTTCCTCTTGTTGATGGGACTTCAATAGCACCTATTTTTTAAAAGAATTAAGGACATTACCATTTAGACAACAAAGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-10 23:17:51 -05:00AEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPt ASPt B1Pt B1Pt BCTS^ABufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *QTDATA *QT9UDATA *QTDATA *QTDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~\LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS7CP1AMKP1RLG&P1WDNP2AMVP2BAZP2RLRPBAS9lPBAS7vPCONE0PCONC:PDMFPDMF7ZPLOC?NPLOC;bPSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%7;SCAN3SMED SMLtSMPLzSPACA]* SPAC7SSPACA]* SVER4.0SVER ^6SVER,,nScal@Scan3TUBEC6Tmpr<UserphARphCH7qphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPt ASPt B1Pt B1Pt BCTS^ABufT"D6CMNT -CTIDCTNMCTOw&CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *QTDATA *QT9UDATA *QTDATA *QTDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTablFVoclFWO_GATCFeat//l\GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~\LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS7CP1AMKP1RLG&P1WDNP2AMVP2BAZP2RLRPBAS9lPBAS7vPCONE0PCONC:PDMFPDMF7ZPLOC?NPLOC;bPSZE`PTYP.PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT / RUNT RUNT  RUNT Rate RunN""S/N%7;SCAN3SMED SMLtSMPLzSPACA]* SPAC7SSPACA]* SVER4.0SVER ^6SVER,,nScal@Scan3TUBEC6Tmpr<UserphARphCH7qphDYbigphQLcphTRphTR[4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95E3&iL! 34pUiT5*L9M cEbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NH!P$JU)wXFT[l"^:w\O_y1m?(}hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bf+mb~97+A.w}9UyV2@VqA4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#