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R = / J D 6 - L [ M H \ N L - W V H + C P > F T 2 C X c J ; 7 P S X _ N h I ] M \ L Y Z m w E h n d _ Z l v ` ^ l i { b j d ~ l o ^ m \ | d S _ \ h ] a Q H s [ B W b Q Y M c ] S A Y ] r e l | s P l r i ] Q D V X B ` d P I Q S _ 6 F R T @ J N ? d 2 W E E F U K f G c [ \ _ a E P 9 f N _ L C C a M S c D X L X c ] N H r \ O l Q Z W d ` e Y W [ 9 U J @ E L E M R 7 E G B O D Z 5 ` J @ J J ^ @ H 3 L \ O \ R @ T g I \ W [ Z b J Q ^ = E O X _ L a G S G 7 D N 4 K P b l M I 1 S H 4 > [ = > l L _ ` Z i l b i \ ] | j w v p g w s _ l S H i F S T ^ W V L Y P J I T P Y Z W l u ] y s t v n y g d t s y [ v u z { z x m q [ K ^ n Z y c w | n p p z Z h \ ^ Z w f [ D ` n Y ` Z Q T L t [ ` d [ p p Q ] k [ ^ Q b J S } S d Q M K L \ 4 P | z o _ j ^ V z | ^ { ~ } t d R W Z ^ S f l ] r V P \ I O O ` l b _ R 6 J M O E V X ^ _ a M Y _ S ` T c l ` p O Z m i } m Q w v R e ~ } k q f I k d m | y z c _ h \ ^ ] V J T F b > A G V b G j a R m c l c w R e e [ \ v [ e M O Z M N h P W G M J A G 9 Q 7 H Q [ = Y ? Q A I d b J _ X X X ` J < ^ D ? ^ R G J ` f S _ U \ e E I b w T W k d g t } p d v k R [ L h @ J Q ^ F H U P f V \ a Y [ V ` o p h n x a T d h k x Z ] O g Z W a U a a D F E 6 G e L I A > P Z A A 6 v H N S X Q I Q ? R D L S H S Q X G R i B M 4 X e W \ X z | r ^ k b Y g ] l c M S \ F G O d ` N Q P G N L S H H 6 @ 7 K Q K S @ Y 9 = L G P T 0 _ L S C A I > ; 9 M C O Z Q 5 > ? V U P S U a P = P O < 9 R X T R M W M 9 P G k R B U ] E T S P M R V S X Y S D R ^ 3 O Z Z s L Y L \ f r f b { X g c I F P W m C T ` d ` S S . I ] O L 5 . I 5 M P @ L W ? 7 . > D S V [ W O W U ` v 7 X E l d P H F T [ o i k b n { p o t } { X \ l ` _ i e q d Z U h B B D f Z j h i Z h Q [ E @ b D > a x > Q a f X L ; 8 P C V _ \ X R U W F T U N V P 9 I M L A L 8 C A 7 I [ D P D C 9 ; H D 2 = K D k J I L ? [ 7 Q S Y H G > X 9 U W G L V V L Z P V Y T P j T u ^ T b [ i a q K I _ G Q J X M U Z I M ] \ L Z _ a i o \ t J W f i T W h n [ l G Y G l s X = G M ^ a Q M g ? J a c b f N c X N h H b V g e a M | ` b I N [ Q Q M F ; _ O T S G + Q Y U T = N [ G P H X Y K O I L V O c _ 3 [ f ` Q f i e U S c Z K [ E W H V e M P D k M G 8 : > h = r ` _ Q r a P h i q o H A b o V X _ [ < I P 7 ? ; 8 B D I , I 6 + F A 5 A W N J : P Z / 0 K K = : C ; O @ B 5 A V N 7 _ : E ? \ P Y R F I M @ D # I E 5 I H C < c K L E W + I @ > P > < > P = H ; / . Z I U 1 @ D @ Q F 8 = C L G : d S Z V v U D < M I V $ K @ @ H a F J I C K 8 O > > F 5 L K R @ \ G Y P 9 M O ] 8 I 5 C 0 > E F I I H A : C 9 B ; H A < N K \ 8 B T L A G S S F N U p Y d d y h l u X X l S O F Y Y S 1 G = U 8 H B 4 0 ; A S P 1 J Q C 0 E H U D ? E C g Z b @ V Q B I G P S ` h K I Z d e C ; X H 2 I L O 6 P G Y ] F H D M : > T N @ G M = M J J B K [ 4 D I 5 B - Q > c Q P I T E F N S L P 3 6 C o W ; P p g d F H A f F _ d l j g c z x | f e Y t d B l S K T \ E . M N U Z Q W \ U B @ N S 5 H 6 ; N M O 7 R a 1 Q _ [ D F D 3 J M 6 K D 7 \ C : 8 > @ L ? N ` 3 > _ P S P V A I K / D : 4 O L 8 > P E @ < i I f Z = 2 E A P 8 E A F A J 4 9 @ 2 D < C U U F 4 N V < ? b M D J < X V 3 7 F ^ ; 6 > < A ? 6 3 D C : E M J A 3 3 8 < B S 1 D C > 3 , B , 9 ' 2 1 H 8 7 5 H _ G F O + @ M O ` 9 P N 3 . . 7 E H < J 5 L 2 F / 7 Y N ; J 9 H 6 * / G > A 3 P 1 \ L O C F @ , S ? + H @ K 4 ? J 7 : @ O 5 ? 5 6 G 0 E > ] G + F J X ; * D O ; ; . D < E B M M Q C F F ; 0 M 2 ( F ) 3 9 @ ? I H 5 O < R / 2 C B A 1 O D F C , - * C ! M G R C I ^ < O D > 0 b : ( E 7 ; O I C ( 7 7 P B 0 # I ^ Q [ D R J I L , = 7 @ F I ^ A = 6 > B & T 1 @ 3 G I : @ D 9 6 E Q : , M ; - ? Y V 6 K 8 K ) @ F P 8 < = 7 E B = = C C 8 E A S + T K E E 4 E D _ 3 W W V H _ O > ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4840904278a149608b266aca999fa82aSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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4y`Y gB>x% X'6 V o8mV L N,Bs@-M qppL i { L$ l GCCCACGCCGCGAGACGGGGGGGACAAGAGGGGGGCACCACAAAAAGGTGGGACACACCGCCACACCTACACGATCACCCCTTGGAAAGAAGTTCCGGAACGATAGTGGATAGGCGCTTCTGACTTTGGGGATGGATCCACGGGGATGGGGAGGGCCGGCGGGTGGCTGCCGGACGGGGGAGTGGGCCTGCCGGCCTCCCACAGAGGACCCGTGGGGAGGCCGACGCCCGGTGAGCCAAGGACGGGGCGCGGGTTCGCCGCAGGGGGCCAGCGGCGAAGTCGCGGGACCGCTGCTTAACAGGTGGGGGGAAGTCTCGACCGTGGGAGCGCGGCACTTTCCAAGAGGTGCAGGTTTCGTGCTAATGTGCCTCGATGACCCACGGGACAGTGTCAGAGTCTGGGTGAGTTTCCTTACAATGAGCCCGGGGATCCCCGGGCTCCTGACCTTTTGTTTAGTCCCATAGTTACCACACCACGCTGGGTGTTAGTCGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:52 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt W ASPt W B1Pt V B1Pt V BCTS , BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA #< Fx DATA
#< Fx 33 DATA #< Fx y DATA #< Fx # DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ +m LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM P1RL > P1WD P2AM % P2BA ) P2RL ! PBAS PBAS PCON Q PCON d PDMF PDMF PLOC PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC Ax SPAC SPAC Ax SVER 4.0 SVER , SVER , , Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt W ASPt W B1Pt V B1Pt V BCTS , BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA #< Fx DATA
#< Fx 33 DATA #< Fx y DATA #< Fx # DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab ;P FVoc ;? FWO_ GATC Feat . . ; GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ +m LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS P1AM P1RL > P1WD P2AM % P2BA ) P2RL ! PBAS PBAS PCON Q PCON d PDMF PDMF PLOC PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC Ax SPAC SPAC Ax SVER 4.0 SVER , SVER , , Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH phDY big phQL c phTR phTR k#?`DuCdb{wMzmaiv5&oX ,zNH~h9bA3^%^#?p;ZJ}wXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]fuq.$>y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUf_|b|~!|3H6*k-;Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~6>udrV0HJA@vd*W]U_I[Ddg/ P1WD P2AM % P2BA ) P2RL ! PBAS PBAS PCON Q PCON d PDMF PDMF PLOC PLOC
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% SCAN 3 SMED
SMLt SMPL SPAC Ax SPAC SPAC Ax SVER 4.0 SVER , SVER , , Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH phDY big phQL c phTR phTR