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W g h m U b T K T j O X W ` L ^ U _ p R H A Y V W M H f W W C f M k f l r H \ [ n \ M a u h \ V j g b j O > L X C R ~ d ` q l F l s y e l u T e x e t r { v v s x d { m x g c k } e } ~ y ~ m z } ~ u o ~ x w s p o u | e { c o V y h k b T Z _ [ p o ^ e ^ r | j ` ^ t d b h ^ s n n b j u s g p n ^ m Y M _ ` P V b g ^ o b S h L T R ^ ^ 8 c c y W _ f P d b p z U ] Y j C Q Z R \ W O V [ J b R H Y S [ e S P L _ N @ Y V a E O c g d T a J f \ X i S V C H @ b U { W : L j d U \ N H J T k T Y R H A g J K ] S L K J O Q ] i d b N ; ` K W 8 ; K R ^ T V M N k > F : Q c Z d d P R l E j Q H h L G _ S : Q c i n ] Y Y h ] T S b j ^ M \ a Z L ] r E ` H K Q U C 3 Y U Q L 5 P > L f 2 n \ Z ^ ^ S N T ] Y @ ^ R [ U [ J [ Y = d V d Y F V I ] 2 H D n \ X Z ^ U d K U ` ] _ U V ^ P \ K < 8 Y 5 M A T A V Z D H A _ p E ` J P Q k ^ E \ O B [ \ I R d q S H \ R U Y b O N V F 5 c V Z 5 F W M B ; a e E N F X : F Q j G C G P Y N U J D H Q B X W k ] H 4 D R K [ Q W W T S e w k q ^ P ; k ] X ` Z i m w W h _ v o o X Q ` [ R e Z q p g q L k d _ n b l z t s c | ^ f z k l d { j h f | k z | n m i v ~ w f w u t a f | w n } g ^ o e t s | q r g ` g c q R n Y ` l ` d Z e U s S T ] m V d } l Y X Q ` Y v b K Z ` P e V _ C d E X [ A ] W O O K ` V L k R Q e L I T I f W \ X ^ T K D f E Z \ D f ^ W I O K L P @ = Q Q O T i V O S g i T v _ c J @ 0 \ R , O d U L M Y 6 I Z 9 L Z I Q F M R L T 9 D ` , X U Q \ T L r S W h B X f I C O B ? D L [ B _ L > @ E 8 D X [ G C B ` O ^ \ G X 4 M S q M K H R K w R K ; K w [ H V V K ^ V + J I i l Z 9 < U H u Y 5 ; M A ^ g a V Q V _ M f c G G ` m ^ F K X H T V T g E e W F c \ g ? K g ] g Z } S C h b O B V W g \ Q K f f O N X Z = O X C [ a ` Q n r 8 L H C T E R T / C O E V W U H I L D T Y Z G U H b O M I G I \ V M H Y X ` A L o ] c e S Q V ] z o W < c Z b d B E ^ Z ^ U _ Z d E I [ U M 7 F U a \ Q G N ? F C Q ` W N M J X _ ] U K M = P F B C G B I L c ^ _ d X : @ N > [ J S E f E A x W 7 X Z E E X _ W I M h W E W / 8 [ ] X O G ^ R I U 3 Z J H M g S R Q W O R P C Y h W A N ^ _ ] > S C o S M 6 \ b R ; G O V = Y W h n d I _ \ [ B L Q P ` M e C = R A O @ ^ < P ] T _ u U k V T V S h U p \ d T G G i T Q S U ^ R J E H H , T D U i 6 R ` l U W I ? 9 H ^ D = B D c i d f b b - H 5 > H N 7 b O E X H - 0 G : j a N W ^ \ @ Q \ S _ R @ X S Q > Z M S B Y b U J : T Z L H @ d e F 6 X c ^ V U F K K [ U - U < d N & O D B O C K X f _ X X H 9 T P U N O $ I K B ? 1 7 4 N B ? L H > e G H 8 / = G R R Q c _ P U 5 = 9 F a U N S \ P ` ^ i a ; ` @ Y C Y > B S S @ 4 V W I L J H Q H B E V S F P R @ > 1 ? C = @ P O N , A > E E G c a a J M 6 O Z R V _ D W @ N C M V K 2 4 C I < F Z V J H A C ` Z P F L S F ] L G d N c V ^ E W I E W F = Q O b R K 9 D \ f ^ S J = C A I N M B k C X B K V B E Z M H Q S N Y \ R U a @ G Q E @ > H Q f N J P W 7 Z I e D R C I I T g U P % 2 : J E L L K 6 R Z F I : N W B 7 E 4 N S C I R Z Q E I E ^ R Q P A 5 5 A N D Q b H b I F E , T W > ? 8 P P 7 D : W $ X T ^ ] D X J Z W ` ? A 0 B 8 ` Q K X M I & I I 7 C 6 D S H Z t C ] L R 9 6 H L [ P X : 6 B O G - H J 5 ] ] ^ \ R Y L 1 V F @ H H 6 S T 0 V ] : Q S Q I _ e c S X X S Z = D ? O L > K S m ` D Q A J m Q ^ T : 5 U D E @ P : U ^ ` N < T 8 ; G N S 9 A L 8 _ Z [ [ \ L J R J 4 D 4 ) 2 ? > 5 . + * H I 6 O G S ^ ? > @ O ] < N A 7 < h E 2 @ R ` H G G P b V N B V S M C R T K G ] P 6 @ Y R C O : 1 e O X 7 Y @ _ U : F N j < N U S C Z V R 8 J R G 9 Y \ G I @ P C > A \ V 7 F N q : S [ @ J 6 Z H ^ A = @ @ * ) T o m 2 T _ N 3 @ ` G 7 V N 8 F P V b n V N V g _ L T _ m j ; F 9 U X V C B M X N > ] W A @ o W X 7 > E H [ T H R i V I S I D C 7 C > 3 P X I > D T I M S Q M [ _ Y I M @ w Z 6 S J V E E @ A P ` C k L f e ` X h _ H < G I A ] U S ? N > 1 U = A f 1 Z K J Z O D 3 V 9 S K Z d I \ E @ D Q V B Z I @ G Z m V Q [ M N X ; D H 8 K B K H M D D O R > L I F U 6 ] a > Y ; k I > T N L = \ N X . _ Z c i Q P Q Z ? 9 O ] I : B E @ H H M T O F E D c [ T ; K X W E G A ? X U e _ D : G ` J E T T U ? C A S M I P W ] N J M > J N : 9 O 9 A C M K ^ N K R F Q U L S K M K c I I C * T L b d T [ U 5 E : B d 6 E G V J [ W S G F _ S @ P D ? M 4 G G V T p V X X ` P P ? ) < D R K E ( F < = & T S b U S C B W f o b R I Z P U < h D Z C _ M _ j M L P Z R d I D K P W c K ^ R a G N @ X N ` H O A O J F B G R L H 0 _ ? ; H B I B P _ F J X ? b ` L L I C 2 9 F Z \ N C a B ^ J E O I B I 8 L M V J H P 3 3 P > J C B T B H L i ^ D R ; C ; ? P Q O P E U W ? C Y c S W M F ; S D @ I Q B ? W J S M U Q D E N L R Q [ D F M R 4 ` L U Q h W T 4 f _ c R N V H = V G ? H O P D = > G U J c Q O V p D T B @ S F F 2 V J L S u ' D I O M R M G \ Q g ] > N 3 D M j Q Z V ^ M G 2 G ^ R ^ q E 2 G = K 2 D ; 9 E I H P ? f W X . T L f P Q M $ M P H O > J J H [ ? a Q = 2 K X J K X @ I G _ O X [ \ 4 K I L S \ U ` 7 A C U G A H = I < F A K ? D Z G M P R c R c = N m L F f c W H L K N U 9 R U D G I K J . N O ` \ \ S G S P O 4 C F G , 3 Q L J X O A : J - Q W [ V A P < 8 S : 8 C J I J ? J J _ S U Z Z I E E E ` o U Z 3 = B L C C 8 J b S > = F . 9 Q E K Q Y Z \ B P = I P H 8 Z g R = O l Z I I 3 ? , @ W a _ W ^ k M K C D 9 Y 8 G ) I 5 V I B X B d O A = D @ Y A V @ < P N + < L Z L Q U N T Y K Q V < C B f L H a J d P I K 8 C f ^ T < Q B T Y : O 0 @ ? P J X 0 I : M % T J Y : S J K c Q B [ 5 6 2 : $ F ? 9 6 U O > G Y I O J @ N W F I F h 6 S Y M 7 L 6 4 C 6 D W f G ` F E = F T [ < F \ > N D D S @ K 9 P = . Q O E N G H M I > C K E B U Q a : 8 h G < Y ^ L , G Z E D < N 9 I 3 2 X ; T G [ H P M 9 > N ; M T ; K _ T N V U ; H = @ M > Z > / N V e U 4 H 9 G ; I O I a 3 @ Q T 4 K L J P L M O 2 W L M 1 > R I % Y O C Q @ = C K O X ? 9 > R a C ? 1 K O 0 > J = D ' T D Q 3 > H R T : 5 Z X W U , 8 J M A 9 7 0 / D ; \ & 7 D @ D 9 E E S D I ; V j g ] h N > : B ` ] < @ J = L J N 9 a 5 K K C L ; @ / K O V X [ Z F N 0 a [ W D F $ # F Q B 6 S ? F W C I G < O Y E ? ; L @ f @ N A 8 F Y @ S ; H T V B = J W J V U B F 7 ? < N K K Z D L 7 A 0 C 8 A G C G E C M D J R D @ R ? < 1 ] ? R G Q : R : U G K A J = = J K 8 L M B U ) 5 1 I I \ ^ M I V ; a > . F E E ] M : D ` L \ Q G K K H T M 6 C E B A Q d R N 4 < F Q 7 O K C L * D . P R 0 - P Q R @ 4 4 : X < Z W J Y N R I H - . C X C M : C H c M 7 % H < 5 I P a N ? G < a > 1 \ H I B D W ? ^ L = R k I X ? E ; L N < @ F , P V 7 L ? ? B @ R Y P : O A I X _ L D P < S G P 7 @ - M E ? < K U W F H 1 B T L M U X K ? > < = @ . : A D T C F @ E D U F S n > + ; @ O a I K [ F A = < J 1 : A ? = I 7 E @ G M Z T T [ Z J U ] O T K : I P J C N - @ U : 8 N J O ( 0 1 G N 2 J P D J ; D = 7 Z ; M Q M @ ? P g X 6 J K G G 5 7 E G X K Y 9 N Y K \ 7 B 2 4 A . 7 P / K X L > V X = / N G Q N 7 A E G L B W X 8 E A B T ] E J @ S K @ A O g S T U P F A P C M . 6 S E D 4 B S 7 P J 2 B > : . A I ? ? K ; * < - 5 9 . = U @ V O J N 1 [ 5 \ D 6 K 6 > < R < C T B < I K 9 K . ! Z I K A F D [ F F F 5 G b R c ? 8 U ; 7 H P I L J O ^ L Y D U F [ = ; I F ? # H ; G Y P < R 6 F L ? B 7 . C A Q j M E A K J Y < Y @ = S N 6 S J L T U C V O > Q J E P 3 a D ; ^ I I [ % ? E % U M 5 U & K H F X @ K G U ; X T @ V ` ? E ? 1 B C Z l V I ^ \ f I Y 5 U . U Q J ? ; F ? N = S 4 H M M B X A ( D C > L \ r Z A D 4 F L I M H A K G L S e B 3 @ 5 Z < M E L S = H U D V J c @ B B 3 D M E > > O Q ; A 7 C < H C H Z d J F G I 3 \ c b F , R F : - T < F 6 N I 1 = 7 O V ? R U K ] N [ E H K C ; T L < + . ] E K G V S E 7 ; M L 9 & L A D Z 9 2 Q \ D : D b H A N G H L Q Z R D H - J W Q K I M 9 Z , 8 ] C K 8 = B ] 9 M Q W I : Y h 4 W K j G L K @ H ? G R \ H R F E 6 R L H D 7 P 4 ? Q g _ K ? N @ p 1 6 = > F R B T 3 @ P K D N H E ? ? E 8 2 9 Z 7 B D V S D G Z f M L N H : 4 G P . F ) R P G 0 = 2 > Q E > 5 + G < ? ^ 2 B ? > T = V C g L L C A $ 5 % 1 C I J X [ H E J @ I B X 0 M Q . 8 D U C G 5 < G S R 6 I U . ( F C K " A B P S I M ] F , N [ > N 9 O O O Y ` N H K < ? M E S P @ D S L 4 @ F E I ; : 1 I @ : D M V @ \ Q F 1 ' S V E F A L H V K D I d 9 F F N > = > U = J ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8b8dcced298d4fda8d45573b4a006585Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-01_10-38_0115 3130Seq 12829 12829_Steier_K1340_psbA jmadera 96-Well
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k)X *= ! p h N CACCATACGGTGGAACCCTTTCCCCTCCCCAAAGTACTGACCTCTACACTGAAAACTCCCATTCCCAACCCATAATTCCATCCCCCTTTTAAGGGGGCCCCGGCGCCGGCCAACCACAACAACGCCTCTTTTGGGGGGACCCCGTTGTCTTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-01 12:26:54 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt m ASPt m B1Pt l B1Pt l BCTS G BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FWO_ GATC GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE @ LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS C P1AM 0 f P1RL 0 6 P1WD 0 P2AM 0 P2BA & P2RL 0 PBAS
PBAS v PCON
PCON
PDMF PDMF Z PLOC 0 PLOC 0
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% ; SCAN 3 SMED
SMLt SMPL SPAC M SPAC S SPAC M SVER 4.0 SVER < SVER , , Scal @ Scan 3 TUBE A8 Tmpr < User phAR phCH q phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt m ASPt m B1Pt l B1Pt l BCTS G BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab $ FVoc $ FWO_ GATC Feat . . $b GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE @ LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS C P1AM 0 f P1RL 0 6 P1WD 0 P2AM 0 P2BA & P2RL 0 PBAS
PBAS v PCON
PCON
PDMF PDMF Z PLOC 0 PLOC 0
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% ; SCAN 3 SMED
SMLt SMPL SPAC M SPAC S SPAC M SVER 4.0 SVER < SVER , , Scal @ Scan 3 TUBE A8 Tmpr < User phAR phCH q phDY big phQL c phTR phTR [4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95\3&ML(g3]OUSc5*79,RVEbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHar$tF)})i%,kc+Q9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wT\3>>B]'p8Npd-(bcn23i8Fbrtr.+6Z=L#=zOE+b.B_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVoJ@GU-SXM6I\(Af73tbT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^i AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt m ASPt m AUDT j j 2 B1Pt l B1Pt l BCTS G BufT CTID { CTNM CTOw CTTL Ȥ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA B DATA & DATA
DATA # G DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN ȅ Dye# DyeN Ȑ DyeN ȕ DyeN Ț DyeN ȟ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT & EVNT 2 EVNT E FTab $ FVoc $ FWO_ GATC Feat . . $b GTyp ! ! d HCFG CE HCFG HCFG HCFG = = InSc InVt LANE @ LAST ~ ~ LIMS ! ! ȭ LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS C P1AM 0 f P1RL 0 6 P1WD 0 P2AM 0 P2BA & P2RL 0 PBAS
PBAS v PCON
PCON
PDMF PDMF Z PLOC 0 PLOC 0
PSZE ` PTYP PXLB RGNm s RMXV 1 RMdN ] RMdV 1 RMdX f RPrN A RPrV 1 RUND
RUND
RUND
RUND
RUNT
& RUNT 7 RUNT
RUNT 1 Rate X RunN " " Q S/N% ; SCAN 3 SMED
SMLt SMPL SPAC M SPAC S SPAC M SVER 4.0 SVER < SVER , , Scal @ Scan 3 TUBE A8 Tmpr < User phAR phCH q phDY big phQL c phTR phTR