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!'''..-555:;;BAAGGGMMMSRRXXX^^^dddiihnnnsssxxz}|~~~~}}}}}}}}}}}}}}}}}}}}}}}}||||||||||{{||{{{{{{{{{{{{zzzzzzzzzyyzzyyyyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzzzyzzzzyzzzzyzzzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyzyyzyyyyyyyyzyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyzyyyyyyyzyzyyyyyyyyyzyyyyyyyyyzyyyyzzyyyyyyzyyyzyyyyzyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyyyyzyyyyyyyzyyyzyyzyyyyyyzyyyyyyyyyyzyyyyzyyyyyyyyzyyyyyyzzyyzyyyyzzzzzyyzyyzzyzyyzzyyzyyyzzyzzzzzyzzzzzzzyzyyzyzyzzyzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz{zzz{zzzzzzzz{zzzz{{zzzzzz{{{{zz{{z{{{{{{{{{{{{{{{{{{{|{{{{{{{{{|{{{{||||{||||{||||||||||||||||||||||||}||||}}||}||}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}}}~}~}}}~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 332fe43ec6a4456eab4e0788d83006aeSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-01_15-45_01183130Seq1282712827_Steier_K1337_psbAjmadera96-Well 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'3@QdHbKU@5K*(M^V7%  E\LL 7CG0*)!8 =3@A":;6Q^+<2.E' O K %;@ ^ &  )v&-L t;U#A %M tW4F,Q)d~~{4>cgQ[G*v-@ yzciL(4!fMu*\9vk7w5D)P {VKP@ ^L{#|S3 #Zeqv8.Z^yK *i(\wvyVBe0P3ZwKFQ \}7[E=@|q!O kWn-zn/*4 cjB8-rXh<Fl0+j?)! 4D0',f-6,!-^ '' , DFd6  #M,-4,$  " + 0 $/ Ve `Bapp :y(5u?m9EG..na&lK (aC'#{A4Erlv5s1nNj4 vk$}2Hp,u D(ZddH*srk9 8~j-)LkG" EuR o t 9 j i(ld*CD qYL:vULKK`JzkR7`hrC^U^+8@,,#MJ HW~ Ymhw=6JK?WO<b>bjK~MBo`4Do0~7E4\96R7XycH+u*8q@UI!Y(o7\\"/dQ8\k$T*EZI0]hT)L?Mr764-@2uQnPX8CJOcx}UZYLf>iHW^b{_]RpkDT^1oFp`LaS4GGACTGGTATCCAGATTACCACCCAAGGAAGAATACATACAGATAACGGCATATGACACCTACAAATAATGAATTATTCCATAAAAAGGAGAGTATCTGCGCCCAACCTCTTGACAAGGCCCCGCAGCAGGGCTCCCTCCCTCGTGACACATTTTGACCAAACATAATTAGTCAAGAACATGGGCGTACCGAACAATACGAGGAAGGCTCACCGAGTGGTTTGTTGGTCTTCCCTTTCAACTAAGGTTAAATCGTTAATTTCATTACAACGTCTTTGTGGAAAAGCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-01 17:15:15 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtoASPtoB1PtnB1PtnBCTS$BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #GDATA #G,yDATA #GtWDATA #G5DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM>P1RL> FP1WD>P2AM>>P2BA|P2RL>PBASmPBASNPCON 'PCON PDMF{PDMF2PLOC>PLOC>PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%SCAN3SMED SMLtSMPLmSPACMSPAC+SPACMSVER4.0SVER SVER,,nScal@Scan3TUBEE1Tmpr<UserphARphCHIphDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtoASPtoB1PtnB1PtnBCTS$BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #GDATA #G,yDATA #GtWDATA #G5DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)FVoc)nFWO_GATCFeat//)?GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM>P1RL> FP1WD>P2AM>>P2BA|P2RL>PBASmPBASNPCON 'PCON PDMF{PDMF2PLOC>PLOC>PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%SCAN3SMED SMLtSMPLmSPACMSPAC+SPACMSVER4.0SVER SVER,,nScal@Scan3TUBEE1Tmpr<UserphARphCHIphDYbigphQLcphTRphTRf\mci2a>qN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`Aq0yYUNNsz)tZv/km]ECGKXW .C5wz^`ZdD\4ApZTFS$oa$Fl@i2go~WR&^.|bEdG;_{8@hh/WZ#3|5O-U[dUDNxGq@p'Ic62v^&Gd\{diO}L6>U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#\f%LXA\%36FM`HIt+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4LHmXY#agsC!.:QI5&.XRz+*}Lq&Wi1*L]W@*q:bXm\(ghkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;=AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtoASPtoAUDTjj7B1PtnB1PtnBCTS$BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #GDATA #G,yDATA #GtWDATA #G5DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab)FVoc)nFWO_GATCFeat//)?GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM>P1RL> FP1WD>P2AM>>P2BA|P2RL>PBASmPBASNPCON 'PCON PDMF{PDMF2PLOC>PLOC>PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%SCAN3SMED SMLtSMPLmSPACMSPAC+SPACMSVER4.0SVER SVER,,nScal@Scan3TUBEE1Tmpr<UserphARphCHIphDYbigphQLcphTRphTR