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E,,,III^^^{{{&&&999DDNXXaaavvv     ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR             EEE??>XXdll{}}     !'''..-555:;;BAAGGGMMMSRRXXX^^^dddiihnnnsssxxz}|~~~~}}}}}}}}}}}}}}}}}}}}}}}}||||||||||{{||{{{{{{{{{{{{zzzzzzzzzyyzzyyyyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzzzyzzzzyzzzzyzzzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyzyyzyyyyyyyyzyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyzyyyyyyyzyzyyyyyyyyyzyyyyyyyyyzyyyyzzyyyyyyzyyyzyyyyzyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyyyyzyyyyyyyzyyyzyyzyyyyyyzyyyyyyyyyyzyyyyzyyyyyyyyzyyyyyyzzyyzyyyyzzzzzyyzyyzzyzyyzzyyzyyyzzyzzzzzyzzzzzzzyzyyzyzyzzyzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz{zzz{zzzzzzzz{zzzz{{zzzzzz{{{{zz{{z{{{{{{{{{{{{{{{{{{{|{{{{{{{{{|{{{{||||{||||{||||||||||||||||||||||||}||||}}||}||}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}}}~}~}}}~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a21f9adca4274d7483bb37949c1bf18aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-01_15-45_01183130Seq1282712827_Steier_K1337_psbAjmadera96-Well K1337_C8_psbAKB_3730_POP7_BDTv3.mobSeqPop7z{ #4I]lttoe\TOKHC<3+'+6GWdhf`]am~zfQ>/()-21)    1CS_m #>XqS~pjjjcP0 9FIKWs0GL@+h?c:'>JLE>;@HOPNOYs%P3c! 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" " ""&.&  23&""    d"-AS0 (BVV=>-0Z1_XMU?= * 3 FJJKb7d3A\ LQ(:$N=@FL&:%OQW$3HM!7;'`'U7P[2[%-/*K''X!UOP(#P>9LJYc#Q''?R?L0X6;Y9R."`U! `;3OY' WRCR^SZcS>2cK%0X`Y`b>ZM`P>-IB'/Q&RL-'0N) %B]M@A_VJ>&E?X-^"('!SdLV$OR)=,?DU6=4TaIZ / C$K;6N>  <NE?RT/+KdBMF 6Z8740S, T`Qa+M=?K>I6>%P<';%bC&FLB^T"+[YG: 4 @J\E18JD0$ O4/<7F>-3&.;Q ;/X[#N<S/&WGWB,.GC_-Tc+K>6ZccP,WV8B.NC$/`*!au\=)1  KE/,ACwm&3x'.CjTD`i P  rLhM]V3? )fB >*#O# mY1g&3iMSn{*`#-kU`EJEL,e\w%Gq/c;^6d|PQya U`Xuhs%UxL&]wT;E< pMH5UC'*7/ : 4-mi-?^kDs6 oZ}SrF<!TnSqq25F5VL-.b3uolTBn'Usv Da`.Jf ^ DkA'}3tQN0eMcU3eD\*aJ0Dg2 #  xt4 1>61J    </ 1d )7$uZ %! nd$&O:  * \ b$W,I   ; M1C1 'Z( >; 4P sd2Kt; <m;  @?7R,?> Y :< kD#3':C%HW.}^e pMe; x&e68 Gki=J;AcS^.}hHIb < vo=:`BlE , p%-zALntvc7MPi<_tVY#e9,L+95YR[iLb\HgTKPC@/HN;ME$Q5Fp`BZ}A 0Hl(@I!T.#,6]C}WY ;r 4c N+<`P~j5S=Qb5,xDuVjbbRp#R4 yd m":5N/%` ;CL+5BHA$' 8sI jbAEd |%Q;-[;\SE1|@2@W4y:[d7vu>cXitv}A-wUYHr,@xV-y01)TW OE/C3@M ^A[ Ou{|yJ<D5AI=];l`C!E{G~;]/mn+?G\k_v[sG=*Jh|k|T.e ~hU{ju)'JCd7Cf]`AP&J?mD.bp7dkX wreJB^OT 1GGAAAAAAGTGTGCGGTTCACCCGAAAAAAAGAGCACAAAAAGAAGATCACGCCCCTCCTATCCAGCCGGACTAAAAAAGGAGGGCACCTAGACATCCACTTTTGATGTCCCCCCTAAACGAGATCCTTCCCAACCAAATAAAGACATAATAAGCCTAAAAATGATGATTACAGAACCCGGGGATTCCCCCAATGGGGGGTAAAGAGTGAACATGAAAGATTTGAAGTAGAAGAAATAACTAATTTAATGCGAAAACAAATTTTGTTCGAACTCCCACAGCGGGTTGTCCATCCAAACATGTGGCAAGGGGCGCTAGAGCATCTACCTTTCGCCAAACTCTTAATATAGCTTGTAGGTTATACAAGAAGAACAAATCCATCTTGATGAACAGAAATGACGAATATAAAGGCAGGTATTAATTCTTTTACTCGTGACTGAGAAGAAGGCAGTAAGGCGGAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-01 17:15:56 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS4BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA %qJDATA %qJ/}DATA %qJz_DATA %qJADCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~2LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+P1AM"`P1RLP1WD%P2AM-.P2BA0P2RL)PBAS+PBAS^PCONPCON,PDMF{PDMFBPLOCPLOCPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%#SCAN3SMED SMLtSMPLmSPACAy+SPAC;SPACAy+SVER4.0SVER 4SVER,,nScal@Scan3TUBEC8Tmpr<UserphARphCHYphDYbigphQLcphTRphTRVThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS4BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA %qJDATA %qJ/}DATA %qJz_DATA %qJADCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabByFVocBhFWO_GATCFeat//B9GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~2LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+P1AM"`P1RLP1WD%P2AM-.P2BA0P2RL)PBAS+PBAS^PCONPCON,PDMF{PDMFBPLOCPLOCPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%#SCAN3SMED SMLtSMPLmSPACAy+SPAC;SPACAy+SVER4.0SVER 4SVER,,nScal@Scan3TUBEC8Tmpr<UserphARphCHYphDYbigphQLcphTRphTRFB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TZc:M?khb"r\vP+h?AxRLp$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*r<4,!:Whj5,9EM8`+MTk0WC.%o,n`!3CS[jWcgnW6Ob#7!PkWFt/kqi~j#RFsx)EF@|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme