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!'''..-555:;;BAAGGGMMMSRRXXX^^^dddiihnnnsssxxz}|~~~~}}}}}}}}}}}}}}}}}}}}}}}}||||||||||{{||{{{{{{{{{{{{zzzzzzzzzyyzzyyyyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzzzyzzzzyzzzzyzzzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyzyyzyyyyyyyyzyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyzyyyyyyyzyzyyyyyyyyyzyyyyyyyyyzyyyyzzyyyyyyzyyyzyyyyzyyzyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyzyyyyyyyyyyyyyyyyzyyyyyyyzyyyzyyzyyyyyyzyyyyyyyyyyzyyyyzyyyyyyyyzyyyyyyzzyyzyyyyzzzzzyyzyyzzyzyyzzyyzyyyzzyzzzzzyzzzzzzzyzyyzyzyzzyzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz{zzz{zzzzzzzz{zzzz{{zzzzzz{{{{zz{{z{{{{{{{{{{{{{{{{{{{|{{{{{{{{{|{{{{||||{||||{||||||||||||||||||||||||}||||}}||}||}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}}}~}~}}}~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 29e6370cf20f479d904eb80346a0501eSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-01_15-45_01183130Seq1282712827_Steier_K1337_psbAjmadera96-Well K1337_C2_psbAKB_3730_POP7_BDTv3.mobSeqPop7z !"#$)1:BEB9/(&,6AJMJC91+&!2b$PEfv &$3:=rA=5){n ) 4tJ`*c5 jO6!jO;0,*#~m\L=1% 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""!%$#3<:/!  %.1,#"-585-&!"&,27<BHOUXYZ^gu &4CQ^ir} &9L]hlllnvqbZ[g{lUC80' sg_\^djnmg\RJGHIHC;2*&%$ zuqqtzzpg_[YWVUVZaioqokhinw~}gQA=DQ\b`VI>6346762,%   ,9?;. $$ "%%      *5=DKT`mzxvz{wuurjZF/    *3871*%#$&%!(14313<L_pxvla^hRm p FH-6 Y Z? v ^  PTf.4]s@CgCM g2[ oyS..GW+G\0 3mAVD@0 ?3A { Bi1vzI%d-94:>?=94.~(#  Tx"\(b=eK5! zvy|wrojd[QJFFHIHEA?AHS_inng\OC:68AObuzhYQQZhyvia^^bju{~{k_XVX\acc`[Y^lynkmomeVC0  !6JXZR@)  &*+' +>NY\VG3  $" !%#&6AC;+    )032-%   0<?8*)/.%    $<Rbkr{;Vl4IXadaVE/^:qcZTOF9)%.1/'             )8FOPI<+  !   $)' $5GVZSB,&/561% $)');LVXPA0  ',+$$&"  "*-)! ")-//-*&   2CKJC=@Ohyi[QLJHC:,   )6?@:/$   !&$+EY`ZH/    %175."  &5DLNIB<840)            %++$              "%! ##  .65,  #**$    )2:=;3& %&!                )01-$  "&"                   !(*$         "((      "*-(  )6=<3%              "$!%4=<2"     "'(% "#  (+'       #'&!         "%#                                                          (--'                                                                            "                                            y\A:VmA:ѷAWMA*0KB.bcpKB_3730_POP7_BDTv3.mobtermTATTACGCCTCAACCTTTTAGAATTCCTATAGAGGTTTTGGGCGTTTGTTTTCCCCTGTTTTCTTTTTTTTTCGTATGAAACTGAGGAACAATGTTTTAATCTTTTAATGTGACTTTTCTCTTTGTTCTTCCCTTTAAAAGTATTACGCCTCAACCTTTTAGAATTCCTATAGAGGTTTTGGGCGTTTGTTTTCCCCTGTTTTCTTTTTTTTTCGTATGAAACTGAGGAACAATGTTTTAATCTTTTAATGTGACTTTTCTCTTTGTTCTTCCCTTTAAAAG*?FYbo|+?HZjs#/;HS[cu&4FPZm|3KYjy%;L[hu,;ENct%/9KVjw*?FYbo|+?HZjs#/;HS[cu&4FPZm|3KYjy%;L[hu,;ENct%/9KVjw                                      `V6%E >>ZI^55DTP"E8U`YH[,96+'UW4a+O 9ABGML^ \L42 <]TVS0U'%>J=b!6^# Y0$J  4/Zbdb! [0+aYCL VN\% *$7,!. IR!`YR3E f ,5FA ad;Z/7 Fr6&pYd;&K +\l  <)Q-" KXTb X Udfh `P79 ( @ ]8P0 *j2 ?pYDzq]r BP*@Epv;|bXL{+(;g@\6@  m+P<i"yl,yq $y:_Dpjv?*,}- %K*P{u8N,"jYjpP&=,1Ax9AoG`7ck&%f+P31b/ ) H C a d R)1 kg{~Qx#Lbc:im}u?26ODXzon@}hT|BiGW1, ].YayK9 ~dmATAACGAGGGGGGGTAAAAGAGGGGGGGGGGAGATCCCATTTTTCCCTCCCCTTTTCCCCCCTAAGCCACCCTTGTATCCCAGAGACGGTCCCTAAAACTATCCAACTAAGCTTCCCGTCTAGGAGCAACTTTCGATTGGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-01 17:15:20 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $IHDATA $IH--DATA $IHuDATA $IHQDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM P1RL P1WD P2AMP2BAP2RLPBASPBASPCON PCON lPDMF{PDMFPLOC RPLOC8PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%SCAN3SMED SMLtSMPLmSPACAwSPACSPACAwSVER4.0SVER SVER,,nScal@Scan3TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $IHDATA $IH--DATA $IHuDATA $IHQDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab!xFVoc!gFWO_GATCFeat..!9GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM P1RL P1WD P2AMP2BAP2RLPBASPBASPCON PCON lPDMF{PDMFPLOC RPLOC8PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%SCAN3SMED SMLtSMPLmSPACAwSPACSPACAwSVER4.0SVER SVER,,nScal@Scan3TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR]%A'on!w.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwN1:^M*92[sgVTzPCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqiH(IwSCmLwV&#g1mo0/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]wclQ[>A{%i/"E.Om,\eyDW"[Fw.eA$^Gj:_]NFJhlyUF_l]hl5cS_d.u?zAw3+fF<7:cFU.F4/~L][U_1(_CcS{[}7"=I).zP{?7.N{Cj_"@?j~J!Eb4;,}E -kb+N4+t457fc|+{Wj2oFtX>j3XV]'p8NpdNmbcn3?GY\U {%KTfx'H9Kx'!byXYhRwZj1& evr.FZH~{8WGic"~9R#1[OIxLqRJ4ir$GG\}d MfaqQl&A6PG>}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTjj/B1PtB1PtBCTSBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $IHDATA $IH--DATA $IHuDATA $IHQDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab!xFVoc!gFWO_GATCFeat..!9GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM P1RL P1WD P2AMP2BAP2RLPBASPBASPCON PCON lPDMF{PDMFPLOC RPLOC8PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT -RUNT RUNT RUNT  7Rate RunN""S/N%SCAN3SMED SMLtSMPLmSPACAwSPACSPACAwSVER4.0SVER SVER,,nScal@Scan3TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR