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A 0 5 8 B B = 0 F 4 K 9 ; G F 7 B O 4 ? ; < B ^ ; < E _ I ^ < g R e k d < F H ; H J I 4 S 3 9 m X _ 5 A B K C 5 H D N 8 T A ; J J H @ F X : Y N ; @ ) H - 1 P E ; 0 / # / b A W K < 1 ? 2 G ? K 3 6 < 4 9 9 : % < M - 0 ! , F ' < 9 ; 6 " 1 @ A 9 / : A E L I 4 = 1 C < F > @ b C \ D O U 4 ; > R L = G S G F [ B R U D > F N K D 1 9 V N C 2 / . 5 * 5 1 B ? % 8 B G 9 R + J M = R . < 3 8 7 9 > / = # F ] B D / E \ h R K O : L L Y r Z B Z b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###***000777===DDDIIIPPPUUU[[[`aeffkkkqqpuuuzzz~~~~~~}}}}}}}}}}||||||{|{{{{{{z{zzzzzzzyzyyyyyyyyyyyxxxxxwwwwwwwvwwwvvvvwvvvvvvuvvuvuvuvuuuuuuuuuuutuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuutuuututuutttttttttutttuttuttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttsttttttttttttttttttttstttsttststsststtttststtttttttssttssstsstsssstssssstsssttttsssssssssttssssstssssststssststtttssstsssstsststtsssstttttststttttststtttttsttttsttttstttttttttttttttttttttttttttttttttttttuttttttttuttuttuututuuutuuuuuutuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuvvvuuvvvvvvvvvvvvvvvvvvvvvvvvvwwvvwwwwwwwwwwwwwwwwwwwwwwxwwwxxxxxxxxxxxxxxxxyxyxyxxxyxyyyyyyyyyyyyyyyzzyyzzzzzzzzzzzzzz{zzzz{{{{{{{{{{{{{|{{{|{||||{|||||||||}}|}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~}}}~}~~~~~~~~~~~~ ! ! ! " " " " # # # # $ $ $ % % % % & & & & ' ' ' ' ( ( ( ( ) ) ) ) * * * * + + + + , , , , , - - - - . . . . . / / / / 0 0 0 0 0 1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 4 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 7 8 8 8 8 8 8 9 9 9 9 9 9 9 9 9 : : : : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 48cc9e41551244be995d0c34bc6f42ebSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well K1315_H12_psbAKB_3730_POP7_BDTv3.mobSeqPop7z # & , 1 6 9 : : ; < = ? B E H J L K H D @ = > A G M R T T Q N J H G G G G F C A ? @ B D F E A : 3 . * ) ) * + + + , . 0 3 4 6 8 ; @ E K N O N K J M U ` m v { z v r q s x | } z s j d c g n t x w s n l m s | ,AWl|oT7 y l a V L C = 8 5 3 2 / - ) & $ $ % ( - 1 4 5 4 1 . , * * , . 0 1 1 / - , , / 5 < C J Q [ l 7g\d YM
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c \ P@A]AKmA $ KB.bcpKB_3730_POP7_BDTv3.mobtermATTTGCCGCTTTGAAGATCATTCTCTATCTCCTTTTCACTCCTCCTTATGGTGGATCTTTTCTTTTTGGTTGTTATTTATTTTTTTTAAAAGGCCTAATAATTTACTTCTTGTATTTTATGCCGCAAAATGCTTTTTCCCTCTTTTTTTTTTTTTTATATTATTTTCTTAATCATAACGATTTGCCGCTTTGAAGATCATTCTCTATCTCCTTTTCACTCCTCCTTATGGTGGATCTTTTCTTTTTGGTTGTTATTTATTTTTTTTAAAAGGCCTAATAATTTACTTCTTGTATTTTATGCCGCAAAATGCTTTTTCCCTCTTTTTTTTTTTTTTATATTATTTTCTTAATCATAACG + C W a o (7@JS^n|
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&3>L\i~ 2?O^o|#0:JW`ny )6@KV`m| *5ANYgr}
'7CM]hu )5@T]o} " . = G
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w?RGGGGCGGCGAAGTGGAGCACACGCTGTCTCTTCCGGGGGATTCTTCATGAAGAAGGAACCCTCCCCAATAAAAATGACTCCCCCAAATCTGCCAACGGAGTACACGCGTCCCATCACAGAAAGCAGGGGGCGCGCGGGTTCTAAAACGGGACACACTCTGGGACGGTAATCGGCGGCATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 11:37:47 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS e BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA #x F DATA
#x F 4 DATA #x F {x DATA #x F h DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ` P1AM f P1RL f + P1WD f P2AM f P2BA ) P2RL f ] PBAS
F PBAS PCON x PCON
PDMF x PDMF w PLOC f _ PLOC f
PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% X SCAN 3 SMED
SMLt SMPL i SPAC M SPAC p SPAC M SVER 4.0 SVER Z SVER , , Ȟ Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS e BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA #x F DATA
#x F 4 DATA #x F {x DATA #x F h DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab & FVoc & FWO_ GATC Feat . . & GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ` P1AM f P1RL f + P1WD f P2AM f P2BA ) P2RL f ] PBAS
F PBAS PCON x PCON
PDMF x PDMF w PLOC f _ PLOC f
PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% X SCAN 3 SMED
SMLt SMPL i SPAC M SPAC p SPAC M SVER 4.0 SVER Z SVER , , Ȟ Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH phDY big phQL c phTR phTR -&/Ur1nsjEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&05t9z"H~:HziP""#]eKZ}X'oM9qB %.}q?AGR(_]u.+I45"KLh]$X>VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f e7{P`+vr4nCe|vH[p!hjqf[MH-YtDSYcJ|_nO*rSC,*cV&hJkcV+9"qsC(Y5_}4]'o>s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52rRU)>`V$];9'k0K3AQZ`K'H:{GKipM`{VL7f4 AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt AUDT l l 4 B1Pt B1Pt BCTS e BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA #x F DATA
#x F 4 DATA #x F {x DATA #x F h DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab & FVoc & FWO_ GATC Feat . . & GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ` P1AM f P1RL f + P1WD f P2AM f P2BA ) P2RL f ] PBAS
F PBAS PCON x PCON
PDMF x PDMF w PLOC f _ PLOC f
PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% X SCAN 3 SMED
SMLt SMPL i SPAC M SPAC p SPAC M SVER 4.0 SVER Z SVER , , Ȟ Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH phDY big phQL c phTR phTR