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C = - L \ H F I Q E 6 N W d 5 . - C F @ G ( = ? M A b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` K J N Y D 9 D I C Y @ F 4 K B D J D F 6 ? O N A T [ A 6 V M D C @ U G F @ ` [ L ` @ = f F 3 O ` E S M E ] M P ^ L O _ ? C Y E G ` E T d 3 d I O @ C D d @ 7 G L ] K = G M X O S J B M A > U L h > N C : ( P J = ; B R ? ; 2 B ' M A * B 0 > D D X 2 ? 7 @ < 6 M 7 > L C : 6 E M P ( 7 / B < . I 2 8 A ; - , / 7 = B 1 C " ( = ? : 3 B E : ' : 8 L 2 F C ( - B A . < % - / ' 1 : 4 1 . $ 5 5 2 ! ' - . 2 - 2 7 I B E L 3 ( 4 . * < J M " @ " 6 E . C 0 3 : 9 J F G # 7 M O ; ? 6 - ? : 9 3 , . $ % 5 9 ) # % 1 , ( . . ' 2 & ; ' 3 ; 0 ! ? 6 ; * 8 6 5 - ) 2 3 ? ( J - 5 , . ; # . & # % 7 / 7 + % / 6 & + ! * ) . 5 $ . # 7 , 9 0 2 ? 8 6 3 6 0 * < M ' - ) 4 <
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d665511b0a3c48ba8bb82558c5ae2ac7Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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" . : NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 11:37:11 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G DATA
# G 4 DATA # G { DATA # G £ DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ' LAST ~ ~
A LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
2 P2BA
< P2RL
( PBAS PBAS PCON
PCON
PDMF w PDMF PLOC
PLOC
PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ȟ Scal @ Scan 3 TUBE E5 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G DATA
# G 4 DATA # G { DATA # G £ DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab $ FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ' LAST ~ ~
A LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
2 P2BA
< P2RL
( PBAS PBAS PCON
PCON
PDMF w PDMF PLOC
PLOC
PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ȟ Scal @ Scan 3 TUBE E5 Tmpr < User phAR phCH phDY big phQL c phTR phTR qk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|@g}V'B<~[V&=zZG&nak'eaK|`h3/[zeqr[&Xj|82Z~9}__';_/e"^=0`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+J-w/l?"ZUDEdi?#vD/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWDZOv'5d7`Ed,{w.P&-Gt?E>@d&r&iox~*4fC\*WiEa7i`8&Ff250bbzE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa$Fl@i2<`2fvn[?a9TghW~^N@.]neZ_kjh'SQ AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN v ASPF ASPt ASPt AUDT j j (7 B1Pt B1Pt BCTS BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G DATA
# G 4 DATA # G { DATA # G £ DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab $ FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ' LAST ~ ~
A LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
2 P2BA
< P2RL
( PBAS PBAS PCON
PCON
PDMF w PDMF PLOC
PLOC
PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ȟ Scal @ Scan 3 TUBE E5 Tmpr < User phAR phCH phDY big phQL c phTR phTR