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T 3 - ( : 1 1 ; V A 2 > J 2 H 2 E g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d I B E 6 * C ; > 0 8 0 L ? O , C 6 D < 5 @ 8 D E G C E / ) 5 8 , = 3 / 7 H @ ? 8 S Q < C f 9 , B @ 2 L D - 6 M G 8 J f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h > 9 < - J 0 8 5 O ; ) 0 9 - 1 @ I C F N N 2 ? A 8 ? 5 H C H 4 & Q ? < 7 T E 8 5 # R I 6 M F 8 < M A 6 A 7 A D M K P A > H J @ 5 8 / G J 3 S : 9 @ ; 4 W 7 ? C P + Z B Y G D 7 D B : 6 7 / E A K B @ I ; B = : 7 ; 0 D X C F S @ 1 F G D , 7 6 = 3 8 L 4 D < ; R ? K * 8 F 3 3 $ I < E > A 5 D J B K 4 / J O 7 6 D 3 * B # N : C 6 A ; F ; 2 J = > 4 G 0 / < . B O 3 6 ! D , G C : J A M 7 6 D M B / 6 , ) 8 ; I 0 L < ; 9 6 / 3 7 4 = & > * 0 , / . 4 8 i / O H 1 E U D 3 7 ? C W 0 ; 6 ; . @ 2 [ C L 7 H = A = F 9 S ) > 3 5 @ E - I C N 3 + A D I N 6 , < * O ? 0 B F 1 9 2 3 . @ < G 6 + 6 B F L 1 ? F M @ I 1 ? 3 0 ) F 6 6 6 9 B ? > M 1 F , * 8 # ( 1 - ; - . B / F A , ? ( 7 ; 9 0 R + ; 1 . 7 ? > 6 G > * : 4 : 2 V , L : ; . D - C 8 7 @ A 7 H > = 9 @ B U G 8 < * 7 F B A 4 ; - C 5 > 2 A , ; < F 5 D 4 8 H / 2 < ? F + 5 ] 3 D . H 1 I < K - E : > 5 3 @ ? 8 F 9 ( K P F . 7 4 5 ' H ' / 6 > 6 E E A 9 K / , C @ * Q G S @ > ? E < 3 F 7 C ' , 7 5 4 + ; 3 5 / B 6 < ? F I ; T 3 7 < ! 8 Q ' = @ ; 8 . E 1 A - 1 B > U ; . 5 : J 7 ) ? ; E A / . F F 6 ; 5 B B K > = 8 F G 2 H 2 > 6 I F ? C L 7 > @ 5 - S > 5 D G 4 E 5 < 5 > ) / 9 0 B < 8 < A 3 P G L M @ E < 2 < C P 8 3 7 ' 4 - 0 : P * @ T A V I = @ = + 7 M < 5 F , = 8 5 B C $ 8 2 8 / O B C = I . B F 9 < 9 J 7 A I > , C 2 I O D 2 3 @ / 9 C , D 8 9 3 6 8 = H M & D ) 4 D N I A C 2 9 5 @ L 9 > # $ C , S L A 1 4 E D E H Z D J C D C H 3 $ F I K D C I ? B > 9 @ < P H - 6 2 - Q @ F [ = 3 A 2 E > @ : 7 2 $ 5 8 G : : Q / @ = 0 ? P W 9 > M @ I H " O K D ; 5 C = 9 C C D B G 0 5 G 8 : F . : 6 A & H M L L J @ $ 6 6 B 0 : < B 0 9 2 7 ? > 5 G C . 2 R : . = > 2 6 K B F 7 2 9 6 2 3 6 5 3 6 8 @ / = Z 6 E L Q Z D = ? E 4 J G E < : Q E L J G < L 2 1 N - F U R H 9 F F 4 - G : = O H U F M P Q ; 1 - S > L G ? G 7 = 9 D - N ? O 7 < 1 G 3 B E ? 1 ; < L 1 C ; 9 T E ; < N I 7 J B 8 B 6 > G B R 7 X E 6 E : = M \ @ E : 2 H 8 7 0 S 3 H N ? E 5 A , = ! C H G D L A E F # M 9 1 8 ; 9 > 6 B K 4 @ - B 9 8 , @ R 2 < O 6 J > I 8 3 = K R 4 N 2 ; : 5 ? : , , < > : O Z 2 - : : R B I 9 1 = ; D = R B L A A 3 E C E . C L A D G L 9 4 ) V / - ' F = 9 3 F D 2 9 K C G O : E ( 2 ? C @ @ Q - @ = < ; F I I K D P / ) ) B B G ; N $ O 6 < F @ ? H < M N 4 E 2 N 6 B @ 3 < D G 8 5 $ H Q 7 a B E > O 3 ; > > D I 9 2 5 / 2 B C E - ? > B 9 J N 8 0 < & ? 2 @ 9 ? W M [ ; + < A A 4 A 6 H C F 0 * N ; B @ B G C 5 > 7 L ; 4 ; Q D O J F H 8 ? ; < I N 7 D * D 9 D E H 4 G 3 L : _ J A I < < J B b J E N \ A J ; E W > H Q C D P ? C = O Z B G 4 ; @ G 8 W > * : ; # ? - T C 4 ; 1 O 3 O U M ? I E A F A 9 M . K @ > @ N > < / O J ? 5 N I @ E * L = A ) B F G ? 8 9 N D B < < @ Q G 5 0 G N @ < A M < . 2 B I I R B P @ F 2 [ W A B = J ? J ; 7 - = < 8 J L = H f G C G ? C N C I @ A 7 # G U V > > ; @ 8 ' = H @ A D ; 6 ! 4 4 > O 7 2 A J @ B ? < > 8 O R F J H G 9 9 C C A > J @ P W K R E L > 1 = C 1 , : L > G S 3 < Y : * < A B < 5 M J D 9 B 1 9 W > I D U @ Y = V [ N & 5 E : 3 B 3 R / C B > D ; D W 6 ) 6 L B 2 9 L N I + 7 D H / A 2 A 9 < U P I E S J @ < . K J B ' ; C / 2 C A > J ; 3 " 9 ; 6 > O H " C F U A R 9 H ` G D = < A M ; 9 B B I 8 5 I D G A ) * 2 9 / M 0 5 K J N ; G H E ' 4 1 9 C 4 U N ? X K I K ? ` : ? ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: af279951b8194f8cae041b3453ccb28aSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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# D ^ i ! c _ t B 6 5 H m / $ ; i 3 7 5 % W z + . H [ [ T M & } d @ ` & / a { j ; $ y d i & 5 ' y ( a w (*K q SCACCACTCTTTACAATATGCGATAGAGTATCAACGACTCACTTAGGACACCTCCTCATTGTGTAGTAGTAACCCCCTTTAGAGGCGGTATAGTAATGGTCAGTTCTAGGTTCTCCCTCGAGAACCCACATTAGTTCTGATAGCGAATCGGTAGACTGAACGGTCACGGTTATAACAACACTACTATAAAACTAGCATGTTTTGAAATAAAAAAAAGGAGTCATCCCCCCGCGGGGACAAGAATAAGTGAAAGTTAATTGCTCCATAAGGATAAAACCTTCCAGAATCAGTAACCCCATAACCACGTAACGGTGCTTGACACCTATGGAGGGAAATCCATGCCGGCCGCGTCTAGAAGGAGGTTGTTAACTAGACATTCAAGAACAAAAAGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 11:36:59 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ) BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G$ DATA
# G$ 4 DATA # G$ { DATA # G$ DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 'z LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS
/ P1AM P1RL P1WD P2AM " P2BA % P2RL PBAS PBAS
b PCON PCON PDMF w PDMF
F PLOC PLOC
r PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N%
' SCAN 3 SMED
SMLt SMPL i SPAC AA SPAC
? SPAC AA SVER 4.0 SVER ( SVER , , Ȟ Scal @ Scan 3 TUBE E4 Tmpr < User phAR phCH
] phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ) BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G$ DATA
# G$ 4 DATA # G$ { DATA # G$ DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 7^ FVoc 7M FWO_ GATC Feat / / 7 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 'z LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS
/ P1AM P1RL P1WD P2AM " P2BA % P2RL PBAS PBAS
b PCON PCON PDMF w PDMF
F PLOC PLOC
r PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N%
' SCAN 3 SMED
SMLt SMPL i SPAC AA SPAC
? SPAC AA SVER 4.0 SVER ( SVER , , Ȟ Scal @ Scan 3 TUBE E4 Tmpr < User phAR phCH
] phDY big phQL c phTR phTR 9pL)Eoud+O'i~o4!KC_K:Zyr-}JIPSX 3-k>"A;%OM5/YME5ts_{'#=g;PFi147nM6!TZ7m8Q`Kp>?0T4`FI57%6C}:*SM|aZL."zr~Mu^yc&w*{we*S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve]^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| VkycEh,MR}*D]d0ov{dk@ /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DbhLYr4EjQI}>uDs5iqbyMimE1ZDh7BjLh|2,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9]CR"lWW .C&q6X+9muy%lFLuLrtrwEU|W{k=0&9 3{ES57hVo+&k=;A{%i|o(>V&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBArpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si! AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt AUDT j j Eq B1Pt B1Pt BCTS ) BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G$ DATA
# G$ 4 DATA # G$ { DATA # G$ DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 7^ FVoc 7M FWO_ GATC Feat / / 7 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ 'z LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS
/ P1AM P1RL P1WD P2AM " P2BA % P2RL PBAS PBAS
b PCON PCON PDMF w PDMF
F PLOC PLOC
r PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N%
' SCAN 3 SMED
SMLt SMPL i SPAC AA SPAC
? SPAC AA SVER 4.0 SVER ( SVER , , Ȟ Scal @ Scan 3 TUBE E4 Tmpr < User phAR phCH
] phDY big phQL c phTR phTR