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X 2 = 0 ! C ] \ F L _ @ , 1 B H 8 Z 3 C 7 + + 3 G H C N : . C 5 4 ( $ 1 > 8 @ C $ = , ? D & 4 : 8 G 4 0 & 4 `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`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b ; > = T 9 ; = : ; H H ? : L : J = = > 8 > @ > " ? > K 1 : 3 8 I I G 8 E : 5 0 C T H M A 4 8 4 = N D 6 * 2 . < B @ ; M < - A D A C 8 O G < 5 E B 5 1 - ( 6 C C H I = & I \ 3 + 6 A I I ? E : [ I ? : C M C P 5 2 3 D : < O @ G ; 6 > ? / F ; & G E L ; < H 8 6 ' 8 ; = K S I = 8 M : = E 7 @ M H 9 5 E 2 @ D F , 8 5 B < J 6 @ / : + 5 9 ? 2 8 5 7 7 0 N E = ' ; ; P B 7 & = A + 1 = ; 9 7 . 6 8 7 3 ( N W E 6 < 6 6 5 / ) F ) ) 3 6 / H 9 : 9 4 7 @ D , O 2 $ ; ( 6 F I 3 M < D 4 U N G C P / 8 5 L 8 7 & 9 3 : 5 E 5 9 6 > " ; - C 0 S > " B J ! 7 8 5 - , : 9 E 2 N I G 1 A I Q , 1 K : 2 @ K / H 8 ? < 7 F E ' 8 ( X 7 S N < 7 = + 0 A J C < F > ' J G 7 @ + 2 ! P 3 B B > B ? 5 D @ 8 B L ; 8 N = 4 , = @ 5 = ? 0 A T . - < 5 ; > @ 9 2 C J = S = D 4 = % 2 : - @ C 9 M 2 * 4 : 5 4 % 8 @ @ P M 4 A K K 1 ( 2 A @ 4 R V 7 1 B * D 5 0 B @ ? 8 3 5 E ? 2 : P 8 2 3 - 0 ! A C 8 F < 4 ( 4 P ' = @ i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i / _ : E 4 F 6 : 4 5 8 0 # H B 0 5 . B 1 9 1 J 2 C ? 3 9 . E 2 + 6 N 4 ' ? E ( 1 , ; . P B D C 2 Y : 8 G 8 9 E C + . K 9 4 D 6 < 3 ; 0 J @ ( 4 ; ) < 4 8 4 R A 8 ; T * 3 F F 1 : 8 K ? ) = 7 J = B D ? ; 6 G @ 1 ) 4 : 7 I ? E 5 G > ; - < 5 C 8 < B T / 5 D ; < 2 5 < G G / 4 ; B / . I C : 4 C 9 8 * 3 ; A 9 A I B ? ) A G > D 3 C ; M " M > 8 > 9 C ; 9 ' E 1 ) ; 9 9 2 P & - ? : B 4 > * 0 7 - 9 4 V 8 4 , 3 7 7 N B / F 4 3 : A % 2 = 4 6 , 4 D ` F + 6 M E J H M D W B I 4 D 4 V O M I 4 ? C + 4 * I J P + 6 < ) 7 H @ C . ? Q B B J 4 . 7 > 7 > A @ J , / F I Q 7 > D = 8 I ? C L ; 8 X R N C < > C 7 G J > A O A > < @ / 5 G = ? F L & 7 R P H < B 9 F > L ? N F H 5 $ S Q O 7 = + 4 6 7 > 6 ? > : 8 ; : 2 D 9 R B : C 8 > 9 C 0 0 % ! B F Q < D < @ = 6 = 9 < - & T B * O @ / = B ; G A @ M @ < B 6 @ 1 C 0 , 5 J O 5 ? 5 M X P 5 G 9 F G E K ? B 5 ; 7 7 L F J @ 7 > 7 + 0 0 K I A / < I O ; 9 A R P ) 6 $ F H J P < J B 5 6 = O ; N K c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` H @ 8 R _ J B ; @ E T 1 I U T B ? C D F M 0 , < I 1 ? Q ? X 9 6 O D : L + B B Q X Z K 7 @ W @ E K . ; 3 B & @ 8 < 8 < 6 @ T @ . J T 9 + 6 ? : 6 F : F G F K 8 9 < < I A C C < A E + 5 T < 0 5 - W . Q 9 8 B = K : K Q J C 5 Q D A 4 B K P = P @ A F = ? F D ? I B ? ? g C ? B < 3 E J C H / B W 8 = B U Y 8 R ? A 9 H G B ; R > 9 W M G B U H S C Q 9 I E L N 5 9 P O 8 D C 0 < S Y = 1 ? E J 8 2 ? % B + < 8 A L : F 6 : I E J ' A A 4 5 9 P - K 9 L ; E Y U D C 2 4 A = ? [ E R = M S T F 2 Z ) 8 @ P Q C Z 6 b - , F A T : U A A P B G B T C > T E L H L X 1 5 C 7 3 7 L 7 R H J P @ M 6 O D E 9 P K < M 5 * M 7 2 = ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ad7bef41be064767b2937679f0a8a40bSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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KB 1.4.1.82018-07-31 11:36:58 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS , BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
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DATA #s F DATA
#s F 4} DATA #s F {c DATA #s F I DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ * LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS 7 P1AM P1RL P1WD P2AM % P2BA )2 P2RL "" PBAS . PBAS j PCON PCON PDMF w PDMF N PLOC z PLOC PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
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RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% / SCAN 3 SMED
SMLt SMPL i SPAC A SPAC G SPAC A SVER 4.0 SVER ,t SVER , , Ȟ Scal @ Scan 3 TUBE E3 Tmpr < User phAR phCH e phDY big phQL c phTR phTR # This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS , BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
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DATA #s F DATA
#s F 4} DATA #s F {c DATA #s F I DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab : FVoc : FWO_ GATC Feat / / : GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ * LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS 7 P1AM P1RL P1WD P2AM % P2BA )2 P2RL "" PBAS . PBAS j PCON PCON PDMF w PDMF N PLOC z PLOC PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% / SCAN 3 SMED
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