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X E G _ @ K > E 6 @ 8 X M I H O X G K H B @ A 6 B . e W X M N L Q D _ g A L P I P I M V E ] V Q i P 7 R M G 6 = ` Q 9 W T J O 5 G A X 8 K F U P B = $ * C O > F H G < D / 8 D D > O M @ O b ? e K P R M A ? W T K @ 1 U 3 G F 9 ; 8 N C 3 H G H F ; G @ ? H O G D O H P E ` 5 W G O E R C U R B I G 7 @ E E ^ < Z = V ] b U D O L P K B Q 8 Y C O E N ; I F P 9 D * I H J N C = L C E H B R G K ; = H K @ N P = W _ J L 3 1 ' N @ < J @ Q F C T O E . X C < A 8 B P M W B 8 J 5 I L E D Q . H D D ; H H > J D S E D > E B O @ Q @ > N ; a R O < E & 2 2 : 0 A 2 E Y a _ < A = 9 : E W 3 G B C F O C O M O A ? G ; J F W c L C L > J C > Y 2 < K = 6 6 ? 3 I Z J < 9 > 4 N G W / B 5 , \ A I D = L N 6 R K O 7 = M Y ; = < < 2 + 4 = U K W @ N h T V C > 8 > = E D S - ? D 5 < H Q 6 @ I D A F F > C S G - I = N D A G = A K J H Q : A B 5 8 A - G ? T = J 4 L H = D P E 3 6 @ < Q A K H < M L Q ^ O H 5 E L N H - * I @ K K G C F : 9 = : ? 8 G N B Q A > S P J L L M 3 U ) E 2 : K ( . ? H 0 ? F A G 8 ? Q * G ? 1 J E C J I D E 7 L G < E I B . 6 R 8 T G Q 1 3 5 = E / C O B i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d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b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b D J C G T . D 5 = @ 9 2 H 6 B @ 9 ; 0 ? ^ L E @ A - 7 K G 1 D & 5 = 1 A 5 9 6 4 D Q N 0 8 C H B B 4 < ) > : B . N < N 9 = J ? Q B 3 Q 8 * 1 J L C , S 6 @ G D ; F - . 0 7 E N M = < B 6 0 I = B I ) F D L M < B 7 E < > L : 8 B L B F ? : < : I 9 Y Z ^ 4 T F K G 0 0 4 8 : 5 - F K 8 P 1 , 1 4 k ? F K N @ J = > A 2 * 9 D * H 4 8 A = ? ' 6 H 4 = @ 4 ? F V ; ; 9 6 [ Q < 1 U H O ? < 9 5 M < < / E 0 ; 6 / < A I E > F 5 ; 9 ; " 7 B K ! , = : B B Y 1 0 ? 2 ; " I / 0 : - 5 A 4 7 - > C 6 + V H P ? 3 E & ( 2 A & ' ? < 3 & 1 4 ? 3 A @ F 9 G Q ' 5 6 B D Y 9 ( S J - H G 3 ; J 9 C B N E A D 9 8 : % * : < U L ; , 1 D E = < 7 P E = D 8 7 C 3 * ? 6 4 > O F ? ( I H < E = P < ( 4 : A J = A ) A B A : 5 D J A 5 5 Q < M % ' < G 8 `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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 29ef3092ee3c49b8b3dda76a1b8dc6e7Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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B 4 k 1 6 8 I F % ? > H 7 4 F C [ > 0 # # @ 8 = S V - m * * ' < / N B A ! ) A E ; c U ] 7 ? , , > a X G y 9 @ [ C S \ 5 X j | B s " S ; T O p - e X m a U D A b c = / 0 L I z % ; U ` k h k ^ \ u ) ( % C , H > * . , q @ & @ 4 W ) - h . : E $ n ' 8 S 4 ? ; k 4 G $ > % X 3 P * N 3 ) * N W \ 7 L > z ^ & R = < 2 M b 5 5 R B d # I \ H J- "CCACTCTATATATATCGGCGATAGAGCGCTACCACTTAAATATGACACCCTGTCATTGTGTAGTATAGAACTGCCGAAGGTACGGTATGGAAGAGTTAGGCTCTCCCTCATGCTCTGTGGAAAATGGCGGGAGCATCGATAAAATACATCCTAACTACTATACAAAGAGTATTGACGAGGTCCTCTGATATTATTGGGGCATTCCAGCCGTCCGTTTCCTCGGGGGTGGGGGGCGGCTTCCAGACTCTATAACCAGGCCTTATCCTATTATAGAAGCTTCACGAAGATCTCGTTTTGTAGAAGGATTGTAAATAGTAATTCTACAACAAAAAGGGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 11:36:45 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt J ASPt J B1Pt I B1Pt I BCTS % BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # Gh DATA
# Gh 4 DATA # Gh |g DATA # Gh DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ $b LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ? P1AM P P1RL P P1WD P 2 P2AM P r P2BA P P # P2RL P PBAS P P PBAS P P r PCON P P PCON P P R PDMF w PDMF V PLOC P PLOC P PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL i SPAC A| SPAC O SPAC A| SVER 4.0 SVER % SVER , , Ȟ Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH m phDY big phQL c phTR phTR J This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt J ASPt J B1Pt I B1Pt I BCTS % BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # Gh DATA
# Gh 4 DATA # Gh |g DATA # Gh DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4F FVoc 45 FWO_ GATC Feat / / 4 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ $b LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ? P1AM P P1RL P P1WD P 2 P2AM P r P2BA P P # P2RL P PBAS P P PBAS P P r PCON P P PCON P P R PDMF w PDMF V PLOC P PLOC P PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL i SPAC A| SPAC O SPAC A| SVER 4.0 SVER % SVER , , Ȟ Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH m phDY big phQL c phTR phTR XPvc.[.Iu2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*27C[%#i$fau?>`:!HI+kmbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@ESLw6i,?P?zwq9Wlwe*vddD\4A,h{|dsgbk1^/.S3xCmqL}4|Md^_w&;H26.?bFW?E)X~6"den?,}&8)TK7\4v3s'kY6C}B-<`}l#[GVsI.J:*yEwiOfFD\4**+^cfoQek>HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX6jJ}Bq#pJE8m+adJs_-ZZ^4n\$0L^#.{glBe+KRCNoE@v#`5E^W6t{_"#k6l2WePm6W.#oU4v_DwW>qO9`}!&RW8X&tG98PbC.Ju5%2-*z$x]f/E03Bvn\N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^