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H 4 3 : J 7 O 1 < H ; 5 O 7 ; Y B N J O G Q U N _ W M O 8 E N J T D F M Y A ; I 9 O I ; > a ^ R A I T I S O X C ^ B H [ b > J E D Q F T L I A _ E B Y R ` , c f W [ J L P P N ] l f Y > O : J H H ] Y N M \ b ` Q V _ h d V m a x Z a e V w u y z X y k z Z x v v | a _ b l l a z ] u t h ` o h U ` q a ^ y ` g i Z T j S ^ b g V b W w v V S Q ] < V r v W Y X S e W N _ W a ] c M E [ I ] N S P ` G F A M O S ; = ; H 8 > 5 R @ K Q J ? ; - > 6 H X ? 6 ; < > D B R = > 5 Z - @ H C 9 C H C 5 I S C F B C 6 6 9 4 6 M B B F I I @ 9 X 8 I S @ L O E H E 8 L ? @ = K K H Q < K < Y E ^ W E L A ; D ? 9 F ; U L H D ? O N > P B L B H W \ B M ( L [ C O Y 8 ; 5 0 N ? D ? U Q H ^ J G G H P = B A I M K 9 K K = P ^ F G D ; R , A A 9 I . B D D 8 9 ; D D D 3 8 7 ; K @ L K F L A : L F 5 k H D 0 : . G P B 2 R ) 8 : F = ) ; 6 3 8 : < I , B < F S O I M a ? 7 1 0 B : H = C 4 O 7 K K F Z U F _ \ U < _ L K B h R 3 ? L @ A @ R R ; L @ 8 V H = = G ? M & A > . < 6 L F A L W I A > : 5 \ > E P * & > N < > ? 4 H Q ? A ' ; 5 + C . F V 8 A @ @ M O 6 B O L < B B I P N < 8 : 6 V 5 I W V K V ? O K 6 H C 3 R = L B Q F C M F ? 4 P N Y _ T V < 6 ^ D W I I P M < = / C B E Z O K Q X N R R = B M D C ? O N J C : J N = @ ? 6 G 7 1 Q Q A Q ? 8 8 ; 2 3 U C = - > F , E O : I J K 9 > I M B 5 3 7 6 ; 7 @ L - T * 6 . 8 C Q 8 A B D B E ? Q O 3 7 T b , Z 8 L I H H C 3 < J 1 K : : - H < 4 U V a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`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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d168f2e656f94cf4b725b115e17d70efSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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KB 1.4.1.82018-07-31 11:36:48 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS n BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA ! B, DATA
! B, / DATA ! B, q DATA ! B, DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS O P1AM q P1RL q
P1WD q P2AM q
P2BA q q t P2RL q
PBAS q q PBAS q q PCON q q PCON q q ( PDMF w PDMF f PLOC q F PLOC q d PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL i SPAC AѨ SPAC _ SPAC AѨ SVER 4.0 SVER c SVER , , Ȟ Scal @ Scan 3 TUBE D2 Tmpr < User phAR phCH } phDY big phQL c phTR phTR l This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS n BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA ! B, DATA
! B, / DATA ! B, q DATA ! B, DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ! FVoc ! FWO_ GATC Feat / / ! GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS O P1AM q P1RL q
P1WD q P2AM q
P2BA q q t P2RL q
PBAS q q PBAS q q PCON q q PCON q q ( PDMF w PDMF f PLOC q F PLOC q d PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% G SCAN 3 SMED
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