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n k i k z j j f ` g N \ b V a f X C V ^ ] R X T O ] Y k H Q _ j ] _ l y z ` ~ p { x .3LJR,2 +Jdp*g4 s f k i \ k X L I N M ` l B 8 A L X I I I ? L T X R J f M H L J l ; I H H _ | O T ] X a c S o z n "Tus$E<R= L+ { w a U t } _ _ M w g ` ^ o Y ] g p Y T _ [ @ D g Z I b ^ L U E > Z O O I b F V \ ? C ? ` Y > = \ e [ ^ [ X ` ^ w ] b i e _ k p n j X Y @ F b = _ s E Z K P ` W T _ l > L [ P [ X ^ k k Z a X S U U U G ; L \ a Z _ U Z V Y R L _ \ \ T M @ O F @ C P O ] H B Y S P U F H 3 B G H L P H G A e T K D K J M e R K E D ^ B A X J W J F > _ = ? N T J [ X \ > N H N Y W K [ ` ^ _ U A L T M ] Y c U \ l q x t l n u k f v u C&4# v t y a V w [ { p e R q h g \ Q a _ V ] W S I J M I O A 5 O J V O d B D ? M Z M N P I > U D K : I M M 9 6 / 0 O E S ? < G I K L C S o O N ` U T m b ` e l o { q z o k _ m o g r j y t m y h l o m \ f e c d W ] X O N B a Y Q X J ; a a K E > ; G F 7 L G B < B 5 A M O 8 E @ K F : L D M 8 D ; I @ H Q C P D M > d J D @ 2 J G \ F J 4 U - E L G W - 6 / 3 4 . > F B 4 G K J D J D 8 : A O F : F F I Z 3 U ; : G H G Q R B T J F Q a [ D M Y V A K C L J I E < Q N ' I C 6 C * @ @ D I C B ? H 1 7 Z 5 D I O H F [ A H G W C N H G M C Q C 6 L E D C N 7 G . A A 4 # % K @ M I E K E O E H ? 2 ? < < K O D < F N D A ; K : 8 E 8 I 5 L H K B 6 L B 9 @ N = I D O : E K 7 R N 5 > ? N ' : < A ? E : ( ? : 9 L @ G ; ? O C F I M T \ I 8 A 8 \ @ H G P L a M K J V Z A > M P = @ ; 6 F ; B C @ F U ^ H L I F : I D D ; Q ? 2 @ F = G < H D A D : 0 > ; 9 C P C 6 R O > K \ \ Q = P O P b z V%T
H e d u [ d _ P S W X ^ p Y L U a D L Q \ W W H 7 N @ D > M D R A U ? ? @ 3 T B < L E I ? O D D S S P H P O E B C D ? I K A 8 = I W S 1 7 5 G ? @ B S @ J N X U N N : I 5 F J J 2 ? \ H E I 8 T 9 B < A F = S ` F P S G G P A > L L R E I L H M P R B 9 C J L 8 I > F G > 8 \ S D E I J I X N S Q C < H ] C B K H F U D ( B K $ 7 A B / 9 J M ] D L = 7 I B @ G F E F 7 M J Q N P T M S U m j g v >e8GkZ5|Q } ~ l k a o [ c u g k M K T ^ I [ P R [ ^ f X Z n ` o s z <s0Tn]Ek% o \ f ` T a V ^ X K ] h O P K K U Y R + M K G 9 J R 3 N J D F < M ; S B U 3 ? 7 D / : > 8 > A I V N E R V Z F V _ V d f 0{Crog,S. u { q t _ Q T h t [ { ~ t k z A_Yim@? "<{@Vw,zD, p a3RckH&v& z q ~ r ] S b h Z H b I M S T O = K Z [ 3 T e I M S R B = P [ C C K H . . 4 2 ; D : D D H H @ M F 8 < > C 6 6 7 H I = E : K : = R ; B M G G J B S E I @ X G ? X @ R R M N 1 ? O B > C > ? J T L K X 1 I B > C E A 6 F A B E C F 9 8 K Q ? I 3 6 ( B P 8 = C 4 4 O F D G D D G L > 1 E H 9 G @ E C o T a l z p ~ j o o g a a ?3" y x x n j d g s ] g | h j f f f H M K \ a ^ b _ Y N ` N Y O F I F l F X 4 J X E = @ 6 9 D I L E C < + . A & 4 0 6 > 5 6 7 E , 9 = ; = M I A ; ? 5 F > B V 0 D T G E K F < 3 A 5 : + D ? D > C > ? 7 & 8 3 5 , ; D 4 C ? ? Y D 9 > 7 R 9 ? I : 5 ; M 3 F A E K F ? B 6 @ @ . A 5 H ? 9 Z B H R P [ O h a ` Z ` e 1}+L[ lmDDHi ,?7g: p z u l \ I Q K D c N ^ W Y d ] T L \ ] c Z K K d T L 9 U E V Q M > A F D L 4 I T Q 1 9 Y J W \ S F N Y J c c h p o D,[zu\'f?9OCnX m u z ^ o m b J N b J B I @ a a T F S D Q S I A : D U . 6 C D 3 5 @ J A F 8 I 1 B ( 4 . - J < 9 3 1 $ C G ? J U @ R I M ? K a k x z '3A6s% } z n s W g i O l U \ a F K K I O V Q P N L M M S W Q ` K U C K L E I N F B / D D ? M < H L D 8 7 A 5 B ? C 6 9 E < < 1 : 2 ) F , ) 4 / ' 6 / 3 ; 7 5 > + 4 ? * = D 9 - - " S E 7 8 @ A ? ? ? 2 < C > / E E P ? C @ D ? E 3 B : 9 > 5 @ 4 P > 7 6 6 7 7 : ? E G J 9 > L D < C @ D @ : B O + @ H C C B 6 . 1 < B 7 F C M ] P D Y a M [ V h e k { E|K_ibw6 RC!5G
a& { j l X U U G S G M O U J \ Q [ N K J D N F V 0 6 K O ? 4 ; = M L / B I < B G I ' < 4 C 4 0 5 F 4 4 = = P _ Y f L E V @ A X Q B E E 8 N * G F - 5 ; 6 1 E * / F A ? B D A > . > 5 R K G B E 3 = I D A @ A > ? 7 8 F F : o 0 E 6 9 M 4 C < < 1 N D 6 R S 2 : A 3 ; @ @ = B 9 > L < F : 6 > B = 1 9 = A @ N ; D ) 5 : 5 : 5 4 B . 6 R 5 8 = 6 4 2 > : 4 = ; * 8 8 D K 1 ; 4 ] G 7 Z V - I = M / C B B 1 ; 8 9 C F 0 < & ; ; g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` 2 Z P @ @ H C U K K f I O F j <{,%}`VW 6:%"S
j g S n V ] c Z e X X Z D H H Z H 9 < 7 O G D L E A A R ? / 6 8 = 7 D % : F = ? H ; < E C 2 E L F L * 5 ? G P 3 O 3 9 : , E 1 : 2 ? : E 4 B / < 6 9 D D B < 8 I 7 M ; C E ; Q F 9 B 1 = C D 4 R G 8 O 0 ) 4 6 & ) = < < 9 2 > D 1 ? @ H V ? ; 2 8 @ 9 ; ; D 0 7 ) 4 2 ; + : 3 A D B ? J . * N A 2 ( C < 9 B , 6 = B D 5 8 : @ 0 D 4 @ 7 D H J ; K / 2 > I 1 ; L ( A = G 7 3 B D J 7 = . - : C D R 4 O ; J ; 8 A L 8 I 7 < 6 H > ; G ? C @ A @ V J G > : : O L : U G _ [ @ M I M T ; L [ [ [ P k m j _ h p u t k q ) ~ o _ q T e p a k g \ [ ^ V V S h e m [ ] s e k W b k k Z J b _ ` d S G R @ ^ U U U S W C \ O \ Y P f S Z H T c ] g f g g b d o p | q t 7>TG8) 8aqlueW.CF9V>z\o:@DN@BNdwuylNVO8!
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 777f5b048cc441ac96389f7f506f66baSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well K1315_C12_psbAKB_3730_POP7_BDTv3.mobSeqPop7z ) ) ( ' % " " % ) , . . , ) % ! # ( - 3 7 8 8 5 2 0 1 5 : > A ? < 7 4 4 7 < A B ? 8 1 * ' ( * - - + ' "
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Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
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DATA ! Cb DATA
! Cb 0 DATA ! Cb t\ DATA ! Cb DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE \ LAST ~ ~ )r LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ( P1AM m P1RL m P1WD m w P2AM m "+ P2BA m m ' P2RL m Q PBAS m m PBAS m m [ PCON m m V PCON m m PDMF x PDMF ? PLOC m PLOC m 5 PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC A= SPAC 8 SPAC A= SVER 4.0 SVER * SVER , , Ȟ Scal @ Scan 3 TUBE C12 Tmpr < User phAR phCH V phDY big phQL c phTR phTR Ɓ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt > ASPt > B1Pt = B1Pt = BCTS * BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA ! Cb DATA
! Cb 0 DATA ! Cb t\ DATA ! Cb DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 9V FVoc 9E FWO_ GATC Feat / / 9 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE \ LAST ~ ~ )r LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ( P1AM m P1RL m P1WD m w P2AM m "+ P2BA m m ' P2RL m Q PBAS m m PBAS m m [ PCON m m V PCON m m PDMF x PDMF ? PLOC m PLOC m 5 PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC A= SPAC 8 SPAC A= SVER 4.0 SVER * SVER , , Ȟ Scal @ Scan 3 TUBE C12 Tmpr < User phAR phCH V phDY big phQL c phTR phTR kl{Zq\-4/CeG1{?{sb: MOsh9VMR5t(jICx@VFmYK3AQZ`K'H:{GKipM`{`3e&Vc:z x:H-p9bgh`U*{Ga$FwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]Z`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ/^idT@CzS[~k8N'O?6pkC:J|bU,iPis|KRaD678w!lVcr,,z^qXnF){3&H9n9f}~E\\&(6Emc9tp] j MfaqQlYm.bBT9zH'zeE^O{U,\)lOY%}WeoVL6e-In(0\5?(,wS:+zB`gVCW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1@ -Tr+ TW>qO9`}!&RW8X&tG98PbC.Ju5%2-*z$I]Ou`Gn'Xu\Mn-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9ci|\Z(dNV*]<4S'dXMTfg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9U|y0Nl~F!,"dI.9L-Y.pa6:!.tkj^4E2rW.X}=_;!;`sEY5_}4]'Z5P%?p MfaqQleA./q}.}:>JWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTcS[7}?e78SG9}_HtV2}2?Yk6]DZSPW~VW<77U AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt > ASPt > AUDT l l Gi B1Pt = B1Pt = BCTS * BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA ! Cb DATA
! Cb 0 DATA ! Cb t\ DATA ! Cb DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 9V FVoc 9E FWO_ GATC Feat / / 9 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE \ LAST ~ ~ )r LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS ( P1AM m P1RL m P1WD m w P2AM m "+ P2BA m m ' P2RL m Q PBAS m m PBAS m m [ PCON m m V PCON m m PDMF x PDMF ? PLOC m PLOC m 5 PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC A= SPAC 8 SPAC A= SVER 4.0 SVER * SVER , , Ȟ Scal @ Scan 3 TUBE C12 Tmpr < User phAR phCH V phDY big phQL c phTR phTR