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G 7 W ? @ 0 B : B 1 % , * ' @ 2 : L 9 L L 3 6 7 , 4 6 Q G ; 0 9 8 ? 3 > ' B < C C 7 H 1 J I B 6 6 D > @ - D 0 9 L J F 9 . 9 9 A F 6 G 9 1 = , # 2 > 3 N L 5 9 = D M R ? L 8 0 G / H : B ; E N > L 6 6 5 = . + , . B & ; 2 9 B ` J E 1 : L A % 5 > * 8 ; 6 * # M 5 P ? ? : ? 8 8 > G / = 5 A H . > 8 Q C , " 9 D S B ; > C 9 8 * B 6 = X ( E G 8 ; . H 5 Q E / ; ( D B = J = A / ' B ) = & E C 6 > 2 9 7 : J 5 $ + ) ; * 9 ' . v Q . 2 8 4 4 2 < 2 5 + C 7 G E 8 G L ? 7 A 6 ? H ) 9 6 # G H ; H E > X ' $ F > : G < & P ? G . , / = A 6 ? 5 A ; F E 2 : G # 5 + 4 & 7 7 ) 9 9 0 - 6 $ 8 = 3 . 2 : ? 1 + R + D 8 3 9 * G - & / * + 1 * 9 W 5 B @ > 1 O E > @ ) K ( A H : K F 6 F : 0 = @ - E B > % > 6 F > < & 6 , = 9 < " 1 C ; ( 1 9 J 2 > > = * ( # 0 & 9 D 3 " ! / - B # L @ 5 - 7 6 8 + / 6 + & , 9 < . 3 , + 4 / ; . 4 < * & Q > - 6 % O > % ( = - ) 1 < @ 6 : 5 : ; 8 8 / ; 2 - 0 7 * 1 D 0 1 ? $ 8 2 : D . 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D 6 $ # * + 2 : 6 ' " 7 6 ? = * 1 E = 7 4 : 6 : 4 / U 2 + 2 $ * * 3 2 5 * < 5 - : & C B . 7 % 6 . E E ' / ' , 6 ) ? 8 ; L + < 8 / . / ' 7 @ 4 I . 9 > 3 2 ) 4 . 9 6 * . ? 5 ' H 0 B 4 3 9 % 9 - / > & * ! ; 3 ' = < ) 8 ' : B , * ) : # * $ ) ) J - 2 9 8 1 0 3 @ ; 7 9 4 > ; < I 0 A : + 0 > A 9 , E 5 F 9 : / 7 Q ) < ? ) ) 0 > / B . ; C ( - ! > ? L D : = < 9 ' E 2 2 : $ 4 3 ? K B 4 & * * ; ' ? , 1 < < $ # & $ 1 B ) G : % / 7 , ) 0 # 3 ? 9 8 7 " 2 . = 5 1 + + : ) ) ? - < 6 0 D - 0 1 : % 7 C A + 7 A * ? ! : 5 2 < 2 2 . 3 C C D . / 5 ) 9 5 , K R - 8 7 D " . + : / 6 $ + 7 1 6 & $ 7 3 . 4 1 4 4 " 5 . ; 0 . C @ / % C A H 9 " 5 4 0 E ? L > , ( $ + 8 # < < 2 ? " 7 . E 9 ' 7 8 5 5 / 5 ' / 4 1 0 8 5 7 5 / : ) 4 8 > 0 + B & ' - 2 0 ( . ( ' * 3 - B ) * 0 ) 2 > * B ? 7 5 & @ . - + / 3 7 > / ( 5 0 ' ' 3 " 4 = + B = / / ( 1 + H = + <
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h $ 1 * 5 * P ' = 3 + + / . 5 , H O H : # @ / 6 * ' 2 7 ( ; : ! & ' ? 2 * 3 5 $ 0 > ; @ / . 0 $ E 8 H 9 2 $ : = A - 5 F ( 8 ) @ ; C " 3 0 5 - - > 6 L < : C ? 1 ( & + 3 , = $ 6 7 7 < - > 1 ' 8 @ : G ( P B 1 C 9 4 # I 7 ? 9 6 , A - T , B : 8 2 2 / M D 9 . / 1 $ : 9 5 5 $ : / 9 / A : 5 4 2 , % , > M A 9 - J # ? 3 P = ; 3 I ( + / * B 2 ; ' ; @ G 1 A / / A 5 ? . J C + + : * 2 6 B A 2 5 N > 1 - 3 = > < 7 5 < E % ' D % # @ 5 / * " + 2 8 " / = 9 ,
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5e428776ffc145268c724cfb073e85d4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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@@ ~AA?AƨKB.bcpKB_3730_POP7_BDTv3.mobtermAAGTTCTCAAGACTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAGACTTTTTTATTAGTCTGAATTTAGACGAGTTTTTGGTTTGAAAGTAAGGGAGCAATATAATTTTATTGATTTTATCAATAGACTTTGATTATTGCTCCCTTACTTTCATTGTTGACTTAAAGTCTATTAAAGTATGTAATAAAATAAATAGAAAGATTTAAAAAAAATTAAAATATATGAAGTAAGTATACTATTATTTACGAGAAGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCAAGCGACAAAAAAGTTCTCAAGACTAGCTGCTGTTGAGTTCCATCTACAAATGGATAAGACTTTTTTATTAGTCTGAATTTAGACGAGTTTTTGGTTTGAAAGTAAGGGAGCAATATAATTTTATTGATTTTATCAATAGACTTTGATTATTGCTCCCTTACTTTCATTGTTGACTTAAAGTCTATTAAAGTATGTAATAAAATAAATAGAAAGATTTAAAAAAAATTAAAATATATGAAGTAAGTATACTATTATTTACGAGAAGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCAAGCGACAAAA ( 0 G [ a r x $0?O`ku &.;ES`iu".8ANZes'5@JUblv&3@LWbo}!,8DQ]ku )6BNXdq}
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w L z M dW , ~ , ns K d3nR a O " lA! ! |ippt5 A Oo CE@p m B8 ! f g p X/ 1 0 ' d g d d% 88 0 i[ gv AB A< X | - : ; Y ` 4 & I ^ t h * A E 6 P = K A E $ ) A U fmgB ' G= D F C 4 I M 1 W l Q { G3 p d > j 4. E q J + i [ p 9 | P e Q" 4 5 `[ < 3 k y # > s 3 ( P # 8 X P ` Y q 3 A - _ n ? / R b j m k H v \ e t r 6 4 v < T G t G % G ; " Y v k . '
T g P L [ Q O \" v TTAACTCTTTACAATATGCGGTGGAGTGCTACGTATGCTTAAATATGACACAGGAATAAGCAAAATTACATTTAACTCGGCCTAAAAAGGGAATCCAAGAATTGCCCTAAAAGACTCCCCACATTGCCTTAAGGTCAGTGATAATGAACTAAGCATAATGGAGAGGGCGTAGCCAAGTGAGTGAGCCGCTGTGGTTAGAGCGTCCACCCTGCGCGAATCCGACCTATGGAGGGTGCCTACCCCTCCGTAAGAGGAACTTCAACAGCAGCCAGGTTGAAGGGGGAATTAGAAGAATGACTCTTTAACTAAACGCCGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 11:37:03 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS & BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G DATA
# G 5k DATA # G }? DATA # G DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ $ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM < x z P1RL < x P1WD < x P2AM < x P2BA < < #Z P2RL < x j PBAS < < ^ PBAS < <
" PCON < < PCON < < PDMF w PDMF
PLOC < x PLOC < x PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC M SPAC SPAC M SVER 4.0 SVER & SVER , , Ȟ Scal @ Scan 3 TUBE B3 Tmpr < User phAR phCH
phDY big phQL c phTR phTR 5 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS & BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G DATA
# G 5k DATA # G }? DATA # G DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4z FVoc 4i FWO_ GATC Feat / / 4: GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ $ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM < x z P1RL < x P1WD < x P2AM < x P2BA < < #Z P2RL < x j PBAS < < ^ PBAS < <
" PCON < < PCON < < PDMF w PDMF
PLOC < x PLOC < x PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC M SPAC SPAC M SVER 4.0 SVER & SVER , , Ȟ Scal @ Scan 3 TUBE B3 Tmpr < User phAR phCH
phDY big phQL c phTR phTR ]VX(p:-"UZ~en?R/FIOb]_C} IBEBW8w|J"Wl&8{[ Mb.$ru$IC6~bTUV5PZGf$ZP}Gs+OStx0Rx;i,sWYT8FBj'~;Hk@*w&RAXWl>jA:>y7$?js3W4KuYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEefwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]ML.Me+bS_?Z p~!*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/W "KLh]$X>VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f7k7:J&q~}JIXEG%w8.2_/ KRZHlEDx8_1tm9i2=#<>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["[87zF4@xSbhhgl5f|&!Bg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW397m@kl+?OR\~[2iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/ AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt AUDT j j B B1Pt B1Pt BCTS & BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA # G DATA
# G 5k DATA # G }? DATA # G DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4z FVoc 4i FWO_ GATC Feat / / 4: GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ $ LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM < x z P1RL < x P1WD < x P2AM < x P2BA < < #Z P2RL < x j PBAS < < ^ PBAS < <
" PCON < < PCON < < PDMF w PDMF
PLOC < x PLOC < x PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL i SPAC M SPAC SPAC M SVER 4.0 SVER & SVER , , Ȟ Scal @ Scan 3 TUBE B3 Tmpr < User phAR phCH
phDY big phQL c phTR phTR