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F F 0 C @ S * 6 ; O 1 G ) D " I " 5 H / B , F \ 9 D 5 F 9 P G K 5 1 > ' : 4 : / 9 L C A 0 C 7 < , < N J B 8 9 D 9 B Q : ? . ? 1 1 6 : 1 + , 6 * C 8 ? J ; 6 2 4 / : + V 1 < * : B 1 2 ; 1 ; @ 8 D I F > ? 9 6 Q G & A 1 1 J ' = J N M : 9 R 5 E + H & ! D ; C 4 C 8 / D < 4 > D - 3 < , : / F A / 9 7 6 3 : 6 8 G V L > ? 6 M L D ; X 8 K I ? L C ) E W ; J 5 ) E - P : 4 > E : 2 4 < X J H E ? : > L `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b I 7 a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` : D D ? O ; < @ 9 8 > 1 5 B > > 4 < K : @ 4 S 1 > A A F 6 2 , 9 G 1 B Q A Q R I B 5 ? ; V ' 0 L T M : R G I U ? ? A < @ > @ : C O > H b L ; : C 9 D / < ) 6 @ A > ' = I < 6 1 : 9 9 : @ ' / O > : 4 C B A C O H B + > < ; W Z G O P B 1 E F G 9 F O a M f G ) O F ? H H R 8 N S H 8 @ > 5 B C 0 = 9 . 2 1 4 F = B = K K > < G = P C C ; @ F ? = I H ; F @ > 2 9 / E D 3 9 0 $ ; 8 ' M , , 4 / E * > 7 = 0 A A = ? A K . & 3 3 A + 0 : * ; : A N C 4 > C * 6 2 & > / 0 : * M Q E M > L 4 0 + M 6 G X 2 ; 4 I B 6 @ : D b G U B G E H @ K , N F G G E R = F @ J 5 F M M 6 7 Q M F M U J J S S B 9 J ; 7 B K K R ? ` N 5 @ A D ; Q 4 / P _ D H @ Q K I @ > C C K K F ` T ` M J d O R < Q L I G G : K a : A H 5 0 [ : D _ K U T 8 Q @ E B N Q ; 1 9 7 ? C , 7 L H A ? [ J S R Q F = a < H 8 N 7 E E ) I 9 B 7 F E G C B K O > B ) ; J 9 H D > V F 1 1 ? < q I C ) 6 I J D 7 @ > < 0 P A @ B / 9 > C ; C R ; G M I 8 ? > < 7 2 = C G D 9 ' 1 9 8 D G ; 4 @ 0 = D < 9 ; : L 2 2 E 8 = ? 9 C 8 5 : G ? - : > 7 E B & 1 F + E , - : . G 7 8 - . = = 2 : 1 K ? + 8 ) - C 8 8 G P B J ] = L < ? < J ; > > L X P H U G C C / F X H I E C G 3 - : A N ; K . M 3 E C @ 7 @ 6 , - L K O D J > F ! M D ? O < E & / = E E B @ 5 * ? ; ; ; A % C 7 @ 8 A = N 3 6 $ 7 = 4 C I 5 S K 7 * L N < < 7 B P > ? A 5 7 R H > A 3 / 0 2 : ? 5 / 1 * 7 = H 0 ; G C D @ 1 P 0 R 1 : K D L ; B F H > Q G H V ] O ; ; L = > , % Q ) 8 0 . G " C ! 9 + J 0 ( ? / 2 7 ( N 2 A : > 1 C > A 8 M V 7 N 3 ; > * X I G E 9 I 4 < 5 D D D ; - P ] K 5 : E E > A 8 > , / + 0 H 9 C 2 ' 0 < E J D : I H J F I A = = E > 7 Q H I @ < ? S @ L C J > 5 L D G @ V 1 I , ) = # 1 1 5 % ) ; ; , 0 * 6 8 A P A . < 1 U N J 9 ? 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' > / @ N 9 V K 4 M ] 6 < F C L B G E > : J G / J N ( @ > @ = O P A @ B I 8 Y G O G C G K ? T A K A V G C ; J 7 8 N E I R H > * 4 ; Q Q 9 < 2 G M ; > M ; Q H ^ = < 2 > ? 7 % W 6 < J L c C W < & 8 ) ? ] S > L 8 J E [ E 1 Z @ E = 6 D = ( - : 8 C K D R > A H V ? G ; 5 D 2 5 T @ F 8 N 7 @ b I U B ; . M 7 7 A O < = J Q P < K D H C A A 8 B I G e = P W ] E M H . C > 5 I L 1 L K V L A V L T 8 G N H K O X G J l c S S X G e N K 8 ] A B 8 K H b E B < R G H 9 G A @ J 6 A 7 > 6 P 6 K P D @ . G D G F D I F K W U ? : K H H F L U R A N < > 7 E 6 F 7 > I S 9 E 4 X G 7 , Q = / @ Q ? L 7 @ M = d E B : 7 M 2 6 D S G 8 N 7 C H 8 @ @ 5 S F 1 K : D : < ) N F . H 9 9 Q 1 8 4 9 A H = , F L - ; F 6 B E 6 W = ; * J A 8 / A V A > G H C S Q I J P F S b : 9 V > L : P N B Y @ 6 3 - E S O L J I J T = 8 < J O 2 M 8 Q M = F 7 S G : > 7 - H @ : W . I A > G \ R C I K @ ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ca50489c14a4490d9526a1f9bbb7533fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-31_09-47_0101 3130Seq 12817 12817_Steier_K1315_psbA jmadera 96-Well
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2y\eTc U NCGCGGTCAACCAAAAGGAGGAATCCTCACTAATCACAGAAATTAGGTTACGGTTGTCATAGTAAGACCCCTCCAAGGGAGTGGGGGAATTACTATATCCTTTGTACTGGGGCCATTAGAAAATGGGCCCTCTACGAGGAGATAAAGAATAGGAGGCTTGACACAGAAGGTGCATGTTTCTTAAGAACACCCACACGCGCCAACGGATGAGATGGCGCGGGATGGGGAGGGGGGGGAGAAAAAGATGAATAGTCATAATAGAAGACTAGGTAGCAGACTACACTTATGATCGGATGAGGAGAGATGGATACGGAAGTCTCACTCGATGTTACAGAGGGCTGTGGCTAGAGCGCATAGACGACACACCGCCGCAAGACGAGGCAAGGACCCCCGGATGTGGCTGGATGGCTAAAGTGTTATTATAGACTCCAGATTATTATAGACCTCAAGTTTAACCGGAAAGCTACGAAAGTGAATAGTGACTCTACAACTACGCAGAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-31 11:36:52 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS +: BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA " E DATA
" E 3C DATA " E x DATA " E DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ) LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS O P1AM P1RL * P1WD P2AM # P2BA ' P2RL PBAS w PBAS PCON 5 PCON @ PDMF w PDMF f PLOC V PLOC l PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL i SPAC Aw SPAC _ SPAC Aw SVER 4.0 SVER +/ SVER , , Ȟ Scal @ Scan 3 TUBE A1 Tmpr < User phAR phCH } phDY big phQL c phTR phTR ' This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS +: BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA " E DATA
" E 3C DATA " E x DATA " E DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 9 FVoc 9 FWO_ GATC Feat / / 9U GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ) LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS O P1AM P1RL * P1WD P2AM # P2BA ' P2RL PBAS w PBAS PCON 5 PCON @ PDMF w PDMF f PLOC V PLOC l PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL i SPAC Aw SPAC _ SPAC Aw SVER 4.0 SVER +/ SVER , , Ȟ Scal @ Scan 3 TUBE A1 Tmpr < User phAR phCH } phDY big phQL c phTR phTR '%N`!2Fu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ou{#\#96q.UtN~EFh%-]3:!h]R_s_beT 1"/"%)O~exg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*#RB63}mZ\Y6G roH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{Z(cyHZ(+i/:uzZ6($UU8>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8Em{s6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(+gbk1^>7(FTcSO[mOEW<#GnP Qc$@)k,g5?w AEPt 3 AEPt 3 APFN APXV 2 APrN ɇ APrV 1 APrX } } ɐ ARTN ASPF ASPt ASPt AUDT j j G B1Pt B1Pt BCTS +: BufT
Y CTID + CTNM 1 CTOw I CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA
^ DATA
n DATA
~ DATA
DATA " E DATA
" E 3C DATA " E x DATA " E DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 9 FVoc 9 FWO_ GATC Feat / / 9U GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ ) LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS O P1AM P1RL * P1WD P2AM # P2BA ' P2RL PBAS w PBAS PCON 5 PCON @ PDMF w PDMF f PLOC V PLOC l PSZE ` PTYP Q PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT $ RUNT
3 RUNT " Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL i SPAC Aw SPAC _ SPAC Aw SVER 4.0 SVER +/ SVER , , Ȟ Scal @ Scan 3 TUBE A1 Tmpr < User phAR phCH } phDY big phQL c phTR phTR