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###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 449a4580a4c24c6ab05fd0a5640d4932Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-Well K1336_H3_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z{.=Nf;m!h& )\:m~ye2|*^*c$sqw~~s[5wt{~jS?43516IPPQYmA|ah   D P  X  0 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}  9 m*bA*XC7?;2* <(`iVP!2 & 1|S/ Cz&}' eVMO  "^m#d3Dj*N]8TPCD[P' 8P &9Ee^fNBYkU*},r`2_Q};?*6Y?\\D# Ds90/A&iulC3TWh0Z$_'D`,m3rx$J. 6KDans"Idkqn$F]m`9i08l. ]`{dHhq/iL %e6{I<4=eS/]tp<.ag0HVu` 'T4FbP>pcu^va4-0JRF\6-nIyWi'<}vwoWG\ EC#e 7  CC{2 #V  f4*+T0FAx]'voNAa)*@MYLF7/ \2' /#/#E,  R/m;!8"/ , !  )0 X 9     *"20$: #6a&N F# 1 <  '  S -[9?  ,! ;s>-VHi(4M+%.Dh]KP/s(T[    <              "    <  <      9  L <<  < )   < <<<<<< < <         j!YE8-6o{.@88C8^xK  II{57;5.q1cLi]js~4dIUm?EolRD,tbS2EPqo3wHWy9['dlGiDMtp!Mu9>g8R(I2Fz*[2MFa zy3%!89g )Cp#y,=&t;|odg},CNV KsC)gd{Z;bzY@1p8HpCd Tgj!6M9%p@B}P29n phpsPp , /EC%3dUFtem[]8<p,N-d  5}F 5 p G".oq9w]k~VdQrregiJ0vhzSGt *Vqy}Aou~wTxv}z\WMw}9ZSwiwj|v>FR `xxu>|lU ;Go-,B51gNl`dGtDy$BFOj@FqSm"f@Vy<ad~'`@ z~3Rr6[5<X~"1  -V^E"lFzEu7X'aUM6kQuaXh6{W!MN0k1n2$7M>zjz.DG+VV:">DOzNWv${*! ep/.o3y^u]-eB|V-16aTt?@i TO6Nj++B qc S'GAAAAAAAAAGCGTGTGCTAAACAAAAGAAATAACATACCATTACTCCATTTGACTTCCTGGACCGGACCACAAAACACTGACCTTAAAGGACCAAATCGAGGAAACTTCGAACCTGGGATTCCCCCTGAAGAAGCCAGGGCCGGGGGTAATTCCCATTCCTCTTACGGCATTTAGACAGATAGGGCGGACCTAGGACTCTACCGCCATCGATGCTTCCAAAGGCGGCTGCCACCTCACCGCACCTTCCTCGGGGTGGACATATCCATAATTCTGTTTAGTACCTATCCCCTTATCCTCCTCTGCAAGGGAATGCGTTACATACCTGCATCATTCAATGGGACGAACTATGGTTGGGACTACAACCCTGCATGCCCCTCTTCATGGATCGATTGCATAATCTTCAGCTTAAGTTAAGACTTGCTAAGGACGTTTTTATGGTAAGTTAATTGACAAAAAGTAATCTAACCAGGGAAGCCGGTCCCCTGGTTTCGGATGCAGTATACCCCCAAAGGGGGTGGGATCCGCTACAATCTAGGTTTTACAAAAAACAATTTTATAAGTTCTTTTTTAAAGAGAATAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:38 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTSHBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 8qTDATA 8qƆDATA 8q8HDATA 8q DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~GrLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMI2P1RLI.OP1WDI7sP2AMI@P2BAIIE)P2RLI<PBASIIPPBASIIPCONII,PCONII)PDMFTPDMFPLOCI%+PLOCI PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL!!TSPACAo)SPACSPACAo)SVER4.0SVER HSVER,,/Scal@Scan@TUBEH3Tmpr<UserphARphCHphDYbigphQLcphTRphTRށThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTSHBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 8qTDATA 8qƆDATA 8q8HDATA 8q DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabWVFVocWEFWO_GATCFeat//WGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~GrLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMI2P1RLI.OP1WDI7sP2AMI@P2BAIIE)P2RLI<PBASIIPPBASIIPCONII,PCONII)PDMFTPDMFPLOCI%+PLOCI PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL!!TSPACAo)SPACSPACAo)SVER4.0SVER HSVER,,/Scal@Scan@TUBEH3Tmpr<UserphARphCHphDYbigphQLcphTRphTR,qJ'8f7j}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"CC+MCUET6D3~x4Gm1lHUNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\cKnY\}wMlhM7y"kVM5`_Q&Ux@~[4mo,*7GP 5Go-D7I 9xxsWD@4ir$GE1Xz.:Axe<>/D?*Uq$[-g]ARc[_8#y-OWVEFiP=MpW\ nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLV:b0;*FbH@O!p8NpdNmbcnPl'Z9dEe/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? M;8'i2{ysPl`A(.:U q<6(mu y-WPv8U)HJxmAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOAUDTeiB1PtNB1PtNBCTSHBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 8qTDATA 8qƆDATA 8q8HDATA 8q DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabWVFVocWEFWO_GATCFeat//WGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~GrLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMI2P1RLI.OP1WDI7sP2AMI@P2BAIIE)P2RLI<PBASIIPPBASIIPCONII,PCONII)PDMFTPDMFPLOCI%+PLOCI PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPL!!TSPACAo)SPACSPACAo)SVER4.0SVER HSVER,,/Scal@Scan@TUBEH3Tmpr<UserphARphCHphDYbigphQLcphTRphTR