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'7</  1!   !    #  '     /  (     51  7?4BE;<G<HA=+&!,7AEK<;0)3G!$,,@II^^^{{{&&&999DDDXXXaakvv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Fcijjjiiihggeeca`_^]\ZWWTSQNNKIHFDCALMMEEEaaawww   ###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f0d226ede1624f4c81f10398c14e686bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-Well K1336_G6_Sanicula_liberta_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zais 7Vdhgjw/j Z0 c'[I # ? n  C ^ ^ K 3 ' . 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JOZ:V^**adM$S0#6Z-AIZJG& C*] 1$ T[)Y' [:M -=C]+O1SN\S5W=$J W7,"GDB -C3/86P=O")H`6'8 :> IX`"8H5aKS^*W7$-d>:;b4DD\M bW^K9 9VCAF\UK:$:6R:VPLDI@E$ @5# Oc6Q `b N=-^J3(:TT9>b'_P@O M#:FZPS +R>WF2SL[# V /*_3aD]$)NQ/Z8H6(-] J5 [6\"G=_]b+EJVZK7LW6Z+>:' *MP-* b),FM6?O1c2:O14WG?"O9$(0[ .NH Z Z8^!'^B_ 7@)>%EU\:ZP 'd2[^)"(X9:N2 a5@\YW YLYAcXFCM 2"d  0X]W^$2K\" ~ ~ } (#^ G\<ASo(^C3wt`2=k0s8zP^MMHr4 nq%I<Q-91Ne%^Ko$>n2} 1v602kDAD4dffjkJ9;V*<Rcr'I 6EbX  2Nv\ M+Y" *xZ&V [|R<U9; hXv\g$ #moBB]cWadaZ 7wRKxIp Hy-D//^#4J[%\K1;n3@11AO= >m~UqK2Z C2^3Q>3}T pf ~~Ec>G*}eu  AI#  XasSp)z Wh HbI*,a"x-e.(`x2 Thla\nS!N!MV/7U_( 4H$ hq )F ( 1) ' &  ( 22V" ##(  #  3",+   D@# '7 ( &P      t2@  ! " KoC O7C8;-% !> <]+87+ B*')B\6a5%(C2Ru}>    ,,/  Y3"9,,,,,  7 77a7,,\9  777977O&7$, >,7,7,R,999,99,9 7  ,,,, 7  ,.bpN8s!Ir?Q[Nd.^Idp! dqk;$} H !I08,K-J,:,>+ \@sq%> ~,sz1\G%NiAVR5Gk`y4G)qDRwBFnuQc@K[4LBb%.Qb&6R]n8>!^y"p:AzzA~oZPNY08z&#1I,,<Dia8!,s }[~v 3f8vp/qfB+G_sp[{ #SyP=6HRlA#dRr&&VKpddd! G > ? Ed + 8]zS`,  W%k]H8yM!&wqI(`V lsosm}'Fe-J;3Es ~iXt}:Z]1 =VCjDRkaNmPun/WFsLyt[0V1qB(p6A=P7xv`bsFpR~5AL^\gq>3a_0p6PxoU/T-pEd WC%xj`TA!=u?Y<M6y7FZD["Gy|U%rnYI:.1%sF_|iEZvt]\qs`Z_EhdMj~`\7:8Vtnu`y=h@%SdemCnbx=@kw|UNkSqAEGvd{>3'h\I]l[vO26iZiJxXv$)N!$j6`2z8&4S<-l!G +wEh]'+IcC6brluT:Y:B=-`v^ wg/bpy2$ *=*+WTTAAGGCGAATATAGGGGCGTTGAAAAAAAACAAAGCACATATCATTACTACATATGCATCACTACATCGAACCACAAAACACCGACATAAAAGCAGCCAATCGAACAAACCTCCAACCCGGCATGCCACAGGAAGAACCGAGGGCCGACCTTAGTGGCCAATCCTCTTATAGCCTTTAGACAGATAGCGCGGACCGAGGACCTTACCTACATGGTTTCCTCCAAAGGAGCTGGCCACCGAGTTACACCTGCGTCCGGGGGCAAAAGTTAAATGTTTGCTACAGGAACTACTCCCTGACACTCCTCTGGAAAGAATTGCGTTAGATAATTGCCTCTATCCAAGGTAAGCACTAAGGTTGGGTATTCATCCCTTCACACTCCTCTTCGTGTAACCAGTTTGAGACTCCGGAGCCTACGTAGAAACCTGCTCCTGCCGTTGTTCTTCCAGGCTAAGTAGTTAACCCCAATGCTGCCGGGCAATAAGGACCCTTTGTCATATTCAGACTTTGCTACCAATTTAGGTGGTAACCGGTAAAAAGCACCTTGAGGCCGATTCATGAATTATCTCCCTTTGAAGTTGGTAAAACATGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:55 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt>ASPt>B1Pt=B1Pt=BCTSJBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9r"TDATA 9r"DATA 9r"9DATA 9r"*DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~IQLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISTP1AMN4P1RLN/P1WDN9/P2AMNBgP2BANNGP2RLN=PBASNNPBASNNPCONNN-PCONNN+[PDMFTPDMFkPLOCN&PLOCN"#PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%LSCAN@SMED SMLtSMPL!!TSPACAm*jSPACdSPACAm*jSVER4.0SVER JSVER,,/Scal@Scan@TUBEG6Tmpr<UserphARphCHphDYbigphQLcphTRphTR(ځThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt>ASPt>B1Pt=B1Pt=BCTSJBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9r"TDATA 9r"DATA 9r"9DATA 9r"*DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabY5FVocY$FWO_GATCFeat//XGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~IQLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISTP1AMN4P1RLN/P1WDN9/P2AMNBgP2BANNGP2RLN=PBASNNPBASNNPCONNN-PCONNN+[PDMFTPDMFkPLOCN&PLOCN"#PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%LSCAN@SMED SMLtSMPL!!TSPACAm*jSPACdSPACAm*jSVER4.0SVER JSVER,,/Scal@Scan@TUBEG6Tmpr<UserphARphCHphDYbigphQLcphTRphTR}0+wU]C.Bv|[Eh2!Ul628z>X}m ;gEF~ba+;x~Cm*qHE?OZC@weCS'J7)Ku3Be%{M*A#t5#r~^;?nU[PbI^6E]bZ&[VGgPH/#+-'M35FQ6n:.]X?K90plql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2WA /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DuYkqU*{Zq9Fy$C.bT8Poo=[/19~{yrA]#_WfOMt_`VWYhY8|kRYN4+HmLP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]SSK.N6jn6O1HnYDx8_b_l4P3LFRt( g>::#Q A0oNm~ |'v9 x&lB9!X4G$$RevlKor5^|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#{HU#9^q.`tNhEe+%-]3:!h]R_s_beT 1"/"%)O~exg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhIGtu6@mV*9w}YE_QqD 4/&;~O2[4mg$VeKD5u/03BSmv^LoT.iP""#]eYWjoW`$~~hY8|kN_uw >xP!t p7qf j O5TwARMASPt>AUDTgHB1Pt=B1Pt=BCTSJBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9r"TDATA 9r"DATA 9r"9DATA 9r"*DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabY5FVocY$FWO_GATCFeat//XGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~IQLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISTP1AMN4P1RLN/P1WDN9/P2AMNBgP2BANNGP2RLN=PBASNNPBASNNPCONNN-PCONNN+[PDMFTPDMFkPLOCN&PLOCN"#PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%LSCAN@SMED SMLtSMPL!!TSPACAm*jSPACdSPACAm*jSVER4.0SVER JSVER,,/Scal@Scan@TUBEG6Tmpr<UserphARphCHphDYbigphQLcphTRphTR