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###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: dc5efd386bd44e3f85620dc5efc9f7a0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_E4_Commelina_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z !,//3<JYdlpvubTLJIHD<2& &O'hc3SXDw^R%5y(t5wh$j)w3}snlllmos| 4` 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KO-^/E0K,(,'E4LbF/E2.2H!%$:+6,%)Df;7.1K6@8L)YI4%92D&+/@4?!+9LQ=21&-%)$XYhd,&0>/+33$K4?),2A6)+:18&(%/C&3*$@*K`!O7+7A5?z21/HUO@=bI<+*1PX7LnOV&cf9;G?GAGAAATATAAGTTCTACACAATAACTCAGCGTATATTATCACCCCATATGGATTCGTGGGCCTAACCCGAAAACACCGTCCTTCGTGGACCAAACCGCGCAAACCACCAGGAGGGAGTGCCGCAAGACGTTGTTAGGGCTGGCGTTAGTTGCTAGTCCTCTCCCGGTCTTCGGAAAGATATGTCGGAAAGAGGTCTTTACCGATTTAGTTTCTTCCAAAGGAGTTTGCAAGCTAATCACACCAGCCTCCGCGGTGAAGGCTCGAAGGTTAGCTTCAGTAGCTATTCCCTTTCACTCCTTTTCAAGGAGAAGGGCTAAGAAGGAGTATACTTCCCTGGGTAGATTTAGAGTTGGGTGTACAACCCTTCACAGCCCTGTTTCAGGTTGAATTGCATAATTCGGTTGTTTATGAGTATCCTGATTATTAATTTTTTATTGTTAGTGAAGAAGGAATAAGTAAGCATACCAGAGAAGAGGGTCCCCAAGGTCGGTGTCCAGAGGATCCCGAATTGGAGTATTATCCCTTTCAATAGCGCCTGAAAGGACAAAATGGGTTATGGCCGGGTGTTGAGAGAACTTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:44 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtEASPtEB1PtDB1PtDBCTSRBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :tTDATA :tDATA :t<DATA :tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~PLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS%@P1AME<%P1RLE7P1WDE@P2AMEIP2BAEENMP2RLEE9PBASEE'PBASEE%sPCONEE5VPCONEE3PDMFTPDMF%WPLOCE.PLOCE)PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%%8SCAN@SMED SMLtSMPLTSPACAkv1SPAC%PSPACAkv1SVER4.0SVER RSVER,,/Scal@Scan@TUBEE4Tmpr<UserphARphCH%nphDYbigphQLcphTRphTR*?This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtEASPtEB1PtDB1PtDBCTSRBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :tTDATA :tDATA :t<DATA :tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab`vFVoc`eFWO_GATCFeat//`6GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~PLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS%@P1AME<%P1RLE7P1WDE@P2AMEIP2BAEENMP2RLEE9PBASEE'PBASEE%sPCONEE5VPCONEE3PDMFTPDMF%WPLOCE.PLOCE)PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%%8SCAN@SMED SMLtSMPLTSPACAkv1SPAC%PSPACAkv1SVER4.0SVER RSVER,,/Scal@Scan@TUBEE4Tmpr<UserphARphCH%nphDYbigphQLcphTRphTRn(_T+D(_7&OMb'~6/lD-EkOptpuD:\E+mH_a2TS7}_'A\3[}k@_aA+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\dxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]4UvI{OF?jp,V0Id4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oX$^(`])>}JcVGkB*Sig]r;a4g0_[YBPxrl@;Gc+[b`[.b=jrOCy~R^bfFc':Z^xDFU)T;w>xP=O_l;mqy=Y`ge#cme/^i1>QD/Px6MkJB1L;,PnRR)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG|@twZ0kz){9a7U9~HK&T|+adJs_-ZZ^kPngD]j;K.LMPS{R2|l|tM%h'_1Z4=KxWTikt72WeKWK2\]G!3@*7_62sF4GG\}d `J8daxW[.b':8 v/qaC^DfFc':Z^STt4)s\T`H%OTKo2W)&p}6irOrm"jTn^@*$9^4ppO{tG 8~#w@Wdgs7_ K]FY{kN{;!Y"%+x]zv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtEASPtEAUDTnB1PtDB1PtDBCTSRBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :tTDATA :tDATA :t<DATA :tDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab`vFVoc`eFWO_GATCFeat//`6GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~PLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS%@P1AME<%P1RLE7P1WDE@P2AMEIP2BAEENMP2RLEE9PBASEE'PBASEE%sPCONEE5VPCONEE3PDMFTPDMF%WPLOCE.PLOCE)PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%%8SCAN@SMED SMLtSMPLTSPACAkv1SPAC%PSPACAkv1SVER4.0SVER RSVER,,/Scal@Scan@TUBEE4Tmpr<UserphARphCH%nphDYbigphQLcphTRphTR