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E                   1                $+('76)#'       '& % L)    +, #  "0010:Xg=CW%!(:9%;3D$;3# " & &    +'1#&; '#E$0$=:7''  ""$!-&-9,$(:    3 ?*D"(-#($    $<")+#$!$.!A%* &!#   ' %  ) 9MIe{`K1:( *,,@II^^^{{{&&&999DDDXXXaakvv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Fcijjjiiihggeeca`_^]\ZWWTSQNNKIHFDCALMMEEEaaawww   ###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3363e7f3015e454cadb582364d2188eeSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-Well$K1336_E1_Triodanis_perfoliata_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z]EE_{aZm %%!4aD^<^jp t+Xghp#^5}K|ja`_T7aHCINH6~hVI?;>Ka~ou9PG ?+-Tp)cx?u[; iToJ+1T{kmo9gVh9"6KZ]R:xP:683|y`gz~c> (QS0z+$-ElmA;U|{F9 '&*<Ws{]/oY@#?luGtkmzysj^QJOc%EijTB6.,++("9^}lXH=873)}ZA305=BB;/!   ,7;8-  !! +F^mqhYG:44685-"*9A@4! 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K V a n y   # / 8 F R _ i r    * 5 @ L X c p ~  $ . ; G P ` n v +7BMYfr!-8AOYakv}#1=O\gv ".8@MUhr "/=GQZjv&3BO]js .<GTcr(6BVamx&6AO\ly,<IRbrz    * 7 H X d s  & 1 ? K V a n y   # / 8 F R _ i r    * 5 @ L X c p ~  $ . ; G P ` n v +7BMYfr                                                          "                                                                                "                      J4E/L "J?,;>/aCR.0#E?)&EGa'@c-D*TMS<UNT;;OOYB5 9=\=1T/._\?T\E JG/K862KLS*<_KMJ: c'S?@ SLJ%H*R&5<F+BcUN+VO6MG/:!&;"E "MFP&F$/ =_+Rc LR"$G(V=@ DL.9Y,HL)#)BR?Q_0:/MU&V-6@ 3)$G_."Q 5*T7NaPF<G/``*]!T.!'4"\Va42@\]4b QH0^JJ48-2A8[W_$UG!F.M+(?S_)6; q,  =6fR* Q]d}HaM+5z<lX#Tr9wY$V ~r>>y:d _:~\X;A*^Ll1<lm}r^D7,Ta6O,=9[TQ/Y`,~7Cy%zR)TC#mF,GSfGsIo)`6i7VQ)2'<M#\ ba8HD/TW:nMk2JEQF/K ([HF*{eb xz`33tK 1{N/^HVTWXRhX1O^g0 8B , Q/K](MF  B>F-:'lDE+/' @;3,/:!:D,>qF Q?BT,_m3xG#I,p|8YBL(5;P@9l%CSu-01iTII~qN\iq6 P p`d3fW+v, +V 5CdfmY% ;+p""I{*>58W?brK64@7/9c${eiBO-1,[DS5Xs0)dBW(C9 `7fG /T3l\!~;N3s <Sl5m&EdmXs[)GAAcJp? VgtU >Im?|V:h 'Ro5SKxW fBw:tYi(w0#ia-N`b!c_eVt!=Z3q^iRIAP<4M1sY249C\o+A_QWk:n&;uVL'gXaACCTAAGGATTTATTTTTGGCTGCAATACGAATGAGGATACATCCTCCCGAGTTATCAAAGGCTCATGCTCACGAGGTCGCACTAATAATGACCCCAATCAAATCAACCAGCTCGCGATTGGTGCAACTGGACTGTTTCCAGTCATAAGACCGGGATGCTCACTGCTAATAGCTCGGTCCCGGAATACTTCTACACTGTACAGTTGCTACCTACGGGATTGGCAGTACCCCCCGCCATTCGTCGGGGGGCGAGAAACCATTTAATGCAATATTGGATAAACCCGTAACCTCACACAGACCGCGTCTGCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:28 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgB1PtfB1PtfBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 6.l\TDATA 6.l\$DATA 6.l\-DATA 6.l\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~bLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AM5jP1RL5jP1WD5jP2AM5jP2BA55-P2RL5jYPBAS55PBAS55sPCON55PCON55 PDMFTPDMFWPLOC5j GPLOC5jPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%8SCAN@SMED SMLtSMPL%%TSPACAp֟SPACPSPACAp֟SVER4.0SVER SVER,,/Scal@Scan@TUBEE1Tmpr<UserphARphCHnphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtgASPtgB1PtfB1PtfBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 6.l\TDATA 6.l\$DATA 6.l\-DATA 6.l\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab-EFVoc-4FWO_GATCFeat..-GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~bLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AM5jP1RL5jP1WD5jP2AM5jP2BA55-P2RL5jYPBAS55PBAS55sPCON55PCON55 PDMFTPDMFWPLOC5j GPLOC5jPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%8SCAN@SMED SMLtSMPL%%TSPACAp֟SPACPSPACAp֟SVER4.0SVER SVER,,/Scal@Scan@TUBEE1Tmpr<UserphARphCHnphDYbigphQLcphTRphTR-Dq.$+WH x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&rBA <3Ofgis$z=AM=/nzy:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]Eg+x+ojG"GA9r::S#6v%B]fH{_Q+cr[t>&,b|MR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4ZP}Gs,"T'oJ-omMOx+6T,IL|2g5^j4foAH%Wnn>iGuxg'jHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"B[M@{`29w/>rU)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#