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# %&?3  "( !#)' &@2,$&#'!M&  $,"'#"' '&!&))   3, 86<% $953-!(   ,*  )  '*          + !+           ,,@II^^^{{{&&&999DDDXXXaakvv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Fcijjjiiihggeeca`_^]\ZWWTSQNNKIHFDCALMMEEEaaawww   ###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 445deed447b14078bfe90e6aa2643792Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_D5_Dahlia_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z}|&/34227HiI\P.#ck.k]^fjbK- (/, &)#7Xv}D 7[.ljA~?eC#hJ(vnq{<.*|"n+9KyT33RrI}a8FvZSW\[VSXhtmsqmpw|wgO5"1IcxygR>."(;KRM<& ,LoB)!Infgmu<D^L_Wm6:n)dE2*)/<L\gg]I1'8DGA4(!")3;BFJMOLD8*   (5;7+  ,AS\[RB0 3X$wmPPYgt{a 1>EGFB<5-%   $)'  +8@@;43AdPD_V*.|zeH.!.?JG9$  (5?B?81,(%! 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01&a64EY2_;?L' `>I)%-4H$KHZaC6O EYC,:1B!B+>L2Q0LD",") G^*P.OIF60J7'd )[Z'BVD b.G8?D ;- P*PS\D 2GD  THF HL+#*NNX)O;M_ %W1-BO 8alf2-M8Uw$Pv  f`e&r> uIfu Jcyz& sFH@ ~!tHb,-;cB5@T Bk%O.EYM:O3SzEEnN K1ybirT]m" o)iGb>fW Z|(m 7IG(]c}:oI]# ucZ<^" 'Y4xV g:*&MM"x3fBn vwI&5p`>R&{{GeSsHguE[vt4o0/a}ow[&<kw&?SoaeWW4$Quse[m)`ncO>49<2 O\ NjpDPS<d{BFoz[{0LJou4S2 ~L5l&{k!Y.M 06h&[:{M<XnM'{_Dua7k1Bo1D EG 6;,1b '+&5=(6$ 7   <0  9   "  ,L9+f ;H'    ' O .  89 !1#C Y&36/+@. 0Ib6" $R Jf "EV( :O,u%     W6>6(   ' D5<)     w ( @  < <<<7< ( 8^Xp>_ppY L*ph}t!*iTdG;8dA8pSI'9iZ`,t#:V8p67p|>%McX"+jpZ:(~n:$pY]8Kx.xzd98pjHj* 3iV Acr<M'3T %v,Z& =R.q54vf`-z9Y>zXm|p8c@(,pvCuG0k9DrTZFTA0 (hqqx`pRq]%gf Z!pAI[3C8)Qwrbo i \d<pD,8 *' Iy Z y 1Gh>WI7Q|XC|f>l8W5]K t@!I8Xc.Fff_pz5S$9B#K{p<;@'C2533>FTUC#^}_rTZog*VOI"NzH_'JO+Hp`",~lE+qX90LB;hNvvW}^<sKKUFUh3 i@Ai`3./f9]V-a`_k36:5$wu.dRb@A+d);AHFvWOYzuX@h pcK.Ypx7vw7qhxTV9I7W}bVTN3u^hTTARpr`WsQ]uiLFoe'tY134~h[P_Ut%}VKq{j6X|JLv>>bKQahX-?ReOxnS[KeZ[I_g=E zM2_E_t.0PTsf tYD]yHUR $LijfTSK[CXqW5r"~ Ik<]3ObcA@p(:#^0/4M D y M EA`TwEcTCAAGAAAAATAAAAGGCCTCAACAGATAGAAGAATCAAATATCATTACCCACATTTGGATTAGTGCACCGGACCACAAAACACCAGCCTAAAAGGACGCGCTCGGGGTAACCGCCCATATGGAGATCCGCCGTGAATAGGTAGGCCCCACATTTGCCGGCAAGACTCTTTTATGCTACTGACACATAGTACGGACCGAGGTTTTTTCCTACATAGTTTCTTCTATAGGTCTTTACGTGGTAGTTTCTTCTGCCTCCTTTGGGAAAAATTAAAAATTTGCTTTAGTCGCCATATCATTAGACTCCTCTGCAAGGAGACGCGCTAATACAGTGTATCCTTCCCCGGGACGCACTAAGGTTGGGAGTTCATCTCTACAGTCAGCTGTTTATGTAACAATTGGATAATCCAATGCTGCAGCTTATACTTGATAATCACGTTTTTCTTCCAGGTGACGTCGTAAAAGGTAATCTGACCAGGTATGCGGGTTCCCTGGCGGCCAGTCCCGAAGATCCCCAATTTGAGTGTTATCCCTTTTAACCACGACTGAAACGGTAGGCAGGATGGACTCCCGGAGTTGATTATAATATCCCTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:40:00 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSiYBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <ywDATA <yH1DATA <yDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;P1AMPSP1RLPN`P1WDPWP2AMP`P2BAPPeP2RLP\@PBASPP>0PBASPP;PCONPPLPCONPPIPDMFTPDMF;PLOCPE PLOCP@PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%;SCAN@SMED SMLtSMPLTSPACAlSSPAC;SPACAlSSVER4.0SVER iNSVER,,/Scal@Scan@TUBED5Tmpr<UserphARphCH;phDYbigphQLcphTRphTR*This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSiYBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <ywDATA <yH1DATA <yDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabwFVocwFWO_GATCFeat//wtGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;P1AMPSP1RLPN`P1WDPWP2AMP`P2BAPPeP2RLP\@PBASPP>0PBASPP;PCONPPLPCONPPIPDMFTPDMF;PLOCPE PLOCP@PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%;SCAN@SMED SMLtSMPLTSPACAlSSPAC;SPACAlSSVER4.0SVER iNSVER,,/Scal@Scan@TUBED5Tmpr<UserphARphCH;phDYbigphQLcphTRphTR8Bb:({.X|L=6XO?Tve!@.%J^K'p=mjzs,t5/`3T6YJ:0/.p9;4^;5s#oC&Opau_l_e5 }37Rgx|ff[`#|D5x_7&OMb'~6/lD-b)jJJTMF4Fi%P p'Icf]4'Uw\Pcc{\A_}$A>AR5Z(yHPx%bg*p/YgnP:Y'e(nNcgp2G316V\7rUj3 t/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuU$HTl:h~6O$@v=aiBx[FohiXN"}a8rOrm>vdA8|+xC,?aU(Zjp~X0&}jk)jA~]/bZ]"Po1Cd}F<_`VWY+a:WkeC.%oh^5Q1V?|&@8je]i=mc~=TtWW; *D/ CoCf3v4*un,T(k"T'ogLOE2(/(W,XD3J6EX! w+[_3sh(2#1WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<AUDTB1Pt;B1Pt;BCTSiYBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <ywDATA <yH1DATA <yDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabwFVocwFWO_GATCFeat//wtGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;P1AMPSP1RLPN`P1WDPWP2AMP`P2BAPPeP2RLP\@PBASPP>0PBASPP;PCONPPLPCONPPIPDMFTPDMF;PLOCPE PLOCP@PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%;SCAN@SMED SMLtSMPLTSPACAlSSPAC;SPACAlSSVER4.0SVER iNSVER,,/Scal@Scan@TUBED5Tmpr<UserphARphCH;phDYbigphQLcphTRphTR