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***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f8ed94e91a3c41968941851857b27b84Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_C4_Clematis_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z"1>M_r)b"3D[~W*.% !:P]^WLA6)Y+zNna_^XG.dD():GNKA2" -S$KCX9bvnG9f b+U  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"  !# (03/' $/3.# ,45/&(13-!  #2;:/ #+28@IQURF4  )3:=:1%  "*6CMOG6 %&  "*)  !%" )010./38<:2$ (00(    &**% /DQO?' )33*!/88..64' 0CQTK8 &+)  /;=5%  (-)   )<HLE6# !  $++#   ,BRVM:" ,694(  (054-"  &-,#        !+264.$ )9EKJB8+    '*' '.244321.(  #*-//.,+)&!   (*%OX]H@GA1'A<A e,KB.bcpKB_3730_POP7_BDTv3.mobtermGGGGACAAAAAACTGCTCAACCATAGTTCTTAGCAGATAAGCCCAATTTTGGTTTAATAGTACATCCCAATAGGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATGCCATGAGGCGGGCCTTGGAAAGTTTTAATATAAGCAACAGGAACTCGCAGATCTTCCAGACGTAGAGCGCGTAACGCTTTGAACCCAAATACATTACCCACAATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAAGGGTAGGCTACATAACAAATATATTGATTTTCTTCTCCAGCAACGGGCTCGATATGGTAGCATCGTCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCCGTAGAAGATTCGGCAGCTACAGCAGCCCCTGCTTCTTCAGGTGGAATTTCAGGTTGAGGAGTTACTCGGAATGCCGCCAAGGGATCAGTATCTTCGGGGACAAAAAACTGCTCAACCATAGTTCTTAGCAGATAAGCCCAATTTTGGTTTAATAGTACATCCCAATAGGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATGCCATGAGGCGGGCCTTGGAAAGTTTTAATATAAGCAACAGGAACTCGCAGATCTTCCAGACGTAGAGCGCGTAACGCTTTGAACCCAAATACATTACCCACAATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAAGGGTAGGCTACATAACAAATATATTGATTTTCTTCTCCAGCAACGGGCTCGATATGGTAGCATCGTCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCCGTAGAAGATTCGGCAGCTACAGCAGCCCCTGCTTCTTCAGGTGGAATTTCAGGTTGAGGAGTTACTCGGAATGCCGCCAAGGGATCAGTATCTTC,4AJV_jqz (5BMYcr}(5ES]iu~ +6DQ_ly)2<HUalv+4ALWbmy (2>HS^jv $2=HT`lz+6CP[gs    ' 3 = H T ` l w    ( 4 @ L X c o z    , 8 C O [ h t  " . ; G S ^ j w    & 1 = I V c o {  "-9DQ^jx*8EQ\iu*5BO[gt(2?LYeq}&5@KXcp|&3?KWdo}'5?MXco|*4ANZgr|&5BMYht{,4AJV_jqz (5BMYcr}(5ES]iu~ +6DQ_ly)2<HUalv+4ALWbmy (2>HS^jv $2=HT`lz+6CP[gs    ' 3 = H T ` l w    ( 4 @ L X c o z    , 8 C O [ h t  " . ; G S ^ j w    & 1 = I V c o {  "-9DQ^jx*8EQ\iu*5BO[gt(2?LYeq}&5@KXcp|&3?KWdo}'5?MXco|*4ANZgr|&5BMYht{   # % )1,464'>8%*'/''&8!*/'2,",'888.'3#+ "++"13;;3.882+(''3;;>3;1;+..+(02(...."$.#.#$$+221;88.$.+)""" (;332'06.28231+6"6;"2(1")'.+.0("''(0 "#%-0.318(0..22,&$+2"13388'3;$$.32#-6.1;882.2'+,.+0.+1++1+;1. -&++(+%%+"&&".#"$"".28(13$""'"  "#"+& "'$$.")(""&""'( "+#  ("'"&(&  1&#+"+2""((" (& 6+"2+"++"& 0$.,""( "  "&)." 0$"'       # % )1,464'>8%*'/''&8!*/'2,",'888.'3#+ "++"13;;3.882+(''3;;>3;1;+..+(02(...."$.#.#$$+221;88.$.+)""" (;332'06.28231+6"6;"2(1")'.+.0("''(0 "#%-0.318(0..22,&$+2"13388'3;$$.32#-6.1;882.2'+,.+0.+1++1+;1. -&++(+%%+"&&".#"$"".28(13$""'"  "#"+& "'$$.")(""&""'( "+#  ("'"&(&  1&#+"+2""((" (& 6+"2+"++"& 0$.,""( "  "&)." 0$"'    )GD R]X1J_F$Q YC UN=:7R)C,L^DMCN \DBJVE< 8YW_0O'1O _LZ?S2[;M;CZGNc (VW7<FRFR9 X Y .*D];] X*C@N69VEJ37PL3 "^J<=@J@ +5J<'6/?#>@9U` -R YFA 1\/E%23DJ .'I$5PBcA,Paa>Uba.V>HOXQ=_T/- 7,1F<TG#3H^[ HRIOJ<.-<P+8>@SD UF&F-C)a>$ M(=1VT:b %:=YK9<TOEQ#->JINb Z$H YGB 7(J95]3Bd0(YD<E9 @4b&!QUZ.< DPa&IX 1 )&&%BN-7Z1,\2XY?Gc29Fa!d&P<NQ'Q"PH8 bKDQWYXM.:8:$aP?Z`I84ZZ#]$IKJ87!< +0cB K_9QTUH;1a 0$k ;yT(0&vB M7~Ke" *r#iY762w oDz *E 6V`t b`p1S~GBI `_uLIFrSu sU 54rP?re8\H}PfUOSva,amg'"u2#?wlY(z~OeJpep 6QB$w?D);0"D:U|Qq#1hOPue8m! Vb {Crj wV'j~kzIFKP  [D4K3O /gWR 0S=zH_~P.b  }<~LOJeMKaWh5:(H/3ELTW<m,99.R/,G0=0J:.%   - #   - B.!@  H+- "C HD&  ZG. %13,MZ>"A<)]&7:8?% 4+ d$/+%oB+_'"b! v00"Kc1]GpzBpppUppx}#mmTppi/?mor8y >)O#"ZFB"Xj5uO\/(i8=86tjQ1R)sgx<-v/U](Huwke*W8<Tb&,;}>V!C[# ! p E NLd8YQF;:i"LCPu  !.*X% ;gAy, ~@HA<!T%`{5;h,~}^6Bu?+G;D\8H:MVW,\#KHNJZ5HZxqR_jvk$n1OdMHQ-P22`WR5Fjs97{?C_qVuON |M.s<zG\Ww}Hk ^6}n?sD2}\a"Hjf[d^\rbEf_;~}aGu5OZ`wCR+|kLq72johpAOy!]'>\[B.p_cM nRy^fw3S+F=CfIhj`FGVW{}7$^utg`'wrligW>(@4/:AACAGGGTTTTCTGTTCATCAACATAAGTCATCACATATTCTTACTAAAGTTGCATTCTTGCACCGAACCCCAAAACAACGACCTAAAAGCGGCAAATCTAACAAATCACCAATCTGGCGTTCCGCCTGAAAAAGTGAGGGCTGCTATTAATGGCCAGTTCTCTTCGGACCTATAGACAGATAGCGCGGGGCGCGGTCTTTACCTACTCGGTATTCTCCGGAGGCACTTGCCCCATAATCAGACCTGCCGCGTGGGGTAAAAATCGAAAATATTCTTTATTACCCATACCCTTAGACTCCTCTTCAACTAAACGCGCTATAAACCAGTATCCTTCCATGTTGAGCACTATAGTTGGGAGTTCAACACAACACTCTCCTCATCCTGGAAAAATTTCAGAATCCGGAGGCTCTATAAAAACTTGATCATTACGATTTCCCTTCAGCTTAACTAATAATAATTAAATTAACTAATGGTGCGCGTACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:48 -04:00AEPt@iAEPt@iAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtCASPtCB1PtBB1PtBBCTSvBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~uLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMdP1RL`RP1WDgP2AMorP2BAs:P2RLkPBASSPBASQ2PCON^nPCON\PDMFTPDMFQPLOCXPLOCTPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%PSCAN@SMED SMLtSMPLTSPACAl-SPACQSPACAl-SVER4.0SVER vSVER,,/Scal@Scan@TUBEC4Tmpr<UserphARphCHQ-phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@iAEPt@iAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtCASPtCB1PtBB1PtBBCTSvBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~uLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMdP1RL`RP1WDgP2AMorP2BAs:P2RLkPBASSPBASQ2PCON^nPCON\PDMFTPDMFQPLOCXPLOCTPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%PSCAN@SMED SMLtSMPLTSPACAl-SPACQSPACAl-SVER4.0SVER vSVER,,/Scal@Scan@TUBEC4Tmpr<UserphARphCHQ-phDYbigphQLcphTRphTR.iTr8p|_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*8ml%RZnKng_ B!Cnq (Vc:=Gk;0|5Q1V?|&@8ja:3AGXS<< xrTW?iMAkc PkIp?H^Poa#]}Ko)6V8JX|K2-!;9xxsWD@|DmOY,f *"49J_'JKxwQ4fC\{lKd#8 UC;2i$@jEl0bRBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziPtJdQex-U[dPz;+hj3T'7_6t;:!8/.Th_sUxiPI^)0F_6`"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"Bm`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt@iAEPt@iAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtCASPtCAUDTB1PtBB1PtBBCTSvBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~uLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AMdP1RL`RP1WDgP2AMorP2BAs:P2RLkPBASSPBASQ2PCON^nPCON\PDMFTPDMFQPLOCXPLOCTPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%PSCAN@SMED SMLtSMPLTSPACAl-SPACQSPACAl-SVER4.0SVER vSVER,,/Scal@Scan@TUBEC4Tmpr<UserphARphCHQ-phDYbigphQLcphTRphTR