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'7HB@D /&( F11.(.:HJRB! 9 &98J/; &:GHOF !   )2G=+$  6- #*>-0  0#*""#(73-,* /&&!)$*+ )!9D    *=@%! 2C32,3 /'%VDI9+ #*2,) (*.:%%!! $"" #'(2*% 8)1  (8631  * !!!'  / "5&.&      $+2)32&08SCBMFMD4-        #:.3'!  <,,@II^^^{{{&&&999DDDXXXaakvv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Fcijjjiiihggeeca`_^]\ZWWTSQNNKIHFDCALMMEEEaaawww   ###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c109f42b6fb94ee19d9bfaf314eddc3eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_A7_Peperomia_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z'8J]rK+U !,4;DMTUQJEFNZdhbTA+[B9?LUP8n[K9!yX9  '36547?HQW[_gr/c`_hOlU@,      =f,?> T? 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'  2'-"     )"     #*3           U3>,3 H A\( #Q6 4X81'1&?@DSZ$,8.P\4%" >(2 - Q MNH;F9 9/5ScZ7@(;')= B]TT?V:,.dOF-R 6!J:VSZ>!/>)J?X"b 5&S`AYb N "/ KCLaY2C GJA(C>: 731KBFF_Z7WU1^U+3:P [W%8BHC&]3 V=EM3 F?&.4X65U W`JAG"4=-?K8M+VU+771%>IU N_"%#44 IZ,8>E3Y1FG43("#+Z7U_`4X]F@EMXC[U_ B^3U26NQ;a6U`,Y="_;OGK, N1KW.>@*NVC6_ \#P,C9'HX[7N_ac$&W8PcDR'9K7]-5 Jd7U08<?*%VU6`\!W%#c W' ab`<dV'*=9$:c'T-`-!,UWaFbJ"4) NMB/) #2NJV4[,\:UVdH%VBa/A36 2 95I4DX(,'b/]2D7,>2\G Rb;\b!P0A02[ KHX~q 6< z`wl#Y2faO->Oz&*z;Ds*I+fj9u." ,ov$M ~a1.~G'VjF4)J1zpVpw@fEkO5 TKfY   cg84* tn(4?&}<5xF$ Efz/ {- X) n/~s=4g!iv}=n9Wm]\/ gt8vApm?MU}:h:;u,pbX:8\5E7pvZB3/Fk^;pg;l<tb+n]ua/O{#sNYkJ1!])0Z yI +qDz5  K)d >`[<]qjeU\?`@h$\~^v` Z] D0Q H Ik]31ni-1@xw-}7wESXL0+*cv @n *!+J>*!17B3&,+bK*'   !5  +   & 3   & )V( /)+*i4 *% >6! 1+%H</d-"*!k"%F5y;$M{M  -#M #%Q:#*******% 1*Z3@*##*##K*6*##*@***K9 8#%******1**>##*r KMpC Bp[p.gdd]Xes=>m$pr%T} -X+*y7uto5 U5I,vP`U0=z !73X*'>\!DgO9*C/}6!A;3ph#n/A psd3an _\nhCAO%p5~2y,Kd*k1R/ )/ 8fLaW8x!ig7L vK'KLj#2_\i= pEb\V8ucQ6pp C) 3sap,Y6N,,Nhpp&afp L:x ?,8xpfLppp5"h.<j1_r_iu>Decy_L1M;ic.(f>_X?TEq@7]f|Z~cJaiC1.G-zIa@o}D,t>:}wS8:Pe8e<uPi}FQi=AhZ'6j[_0zTfFi[)^{LvazcR|pR@Q:M8PCU8SVX7cOq?d+BS\Zdul:ZRX?- Z-V}<oV4eH^-{Vxd`tmes5'cxO2^%U/noB-HCzCnPU~/Oj: WTE9gv,"JUwbVmzHKZpHx~WUgTLeyjgg-jbNrR9nSQc\k(p?nbOo|yF(M-sg_2elmX]j]jmq:`n> utlL^qv)?QpHD Aqlo$ib#} ,iITaOH 1rR>w'f PW/^zZK w/uX7F 3nm^GAAAGGTTAAATCACCGTCTACCCAGAGAGGCTAATCGATTATCATAACCTAAGTTGAATCCGTGGACTGAACCCGAGAAGACCGACCTTAGAGGACGAAGCCGGGGAAACCAGAAAGCAGGGAAGCCCCCCGAGGAAGGTTGGCCCTTGATTAGCAAGCAGTCCTTCCTCGTCCTTCCGAAATTCAAGTTGGTAATCCATCTTAACCTATTTAGTTAGCTGGAACGGCGGTTGCCTGGCAATTGACCTTTCGTCAGGGGGGAAAAATGGAAGATTTTCTTTACAACCTATGGGGTTGAACTCCTTTTCAACTACTCGCTCTACGAACTAGGTTCTTTCCATGGGGCGGAGGTAGGTTGGGCATACAACCCTTCATTCTGTAAGTTATGGAACAATACAATAATCTGCAGGTTATTTCAGATCTTGGTTATTCCATTTTTTTTCCATTTCTTGTAGGTAAACGGAAAGTTGGCACGGATTCGGGTACCTTGGGTTGCGGTACGTAGGTCACCAAAGTAGGTGTTAAACCTTTCAACGAACCTGTAAAACGGACTGAGACTTAACTCCCGGTGAAGGAGGTAATAAAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:40:21 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtTASPtTB1PtSB1PtSBCTS0PBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 5kTDATA 5kJDATA 5k+DATA 5k^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~.LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMLP1RLLP1WDLP2AML'P2BALL,{P2RLL#KPBASLLoPBASLL#PCONLL7PCONLLPDMFTPDMFPLOCL SPLOCLPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACAoSPACSPACAoSVER4.0SVER 0ESVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCHphDYbigphQLcphTRphTR!ÁThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtTASPtTB1PtSB1PtSBCTS0PBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 5kTDATA 5kJDATA 5k+DATA 5k^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab>FVoc>FWO_GATCFeat//>kGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~.LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMLP1RLLP1WDLP2AML'P2BALL,{P2RLL#KPBASLLoPBASLL#PCONLL7PCONLLPDMFTPDMFPLOCL SPLOCLPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACAoSPACSPACAoSVER4.0SVER 0ESVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCHphDYbigphQLcphTRphTR^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+AO5L Ml~5E).'=mghpCq>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@fol\UyYd!i e.*w_+)Yo9H/w=E*A-;Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pf|r,"}&A8uD=O?HS|G;~fw O_Kk9Cdc0Eo8:"]R_s_be1}1"/"E.Om,P>bH6X12g[jWcgiQF4Np3`4 x'gJx%*{#ODYnWZjdO{7vMjL3ro{`N%7`h?I8rBArIUwch[}k@_a'>yj6m}P,PHq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,ComMOxq6agI){y&m"o`^ kS\KQpYp&!'\/.:AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtTASPtTAUDTLB1PtSB1PtSBCTS0PBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 5kTDATA 5kJDATA 5k+DATA 5k^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab>FVoc>FWO_GATCFeat//>kGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~.LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMLP1RLLP1WDLP2AML'P2BALL,{P2RLL#KPBASLLoPBASLL#PCONLL7PCONLLPDMFTPDMFPLOCL SPLOCLPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%SCAN@SMED SMLtSMPLTSPACAoSPACSPACAoSVER4.0SVER 0ESVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCHphDYbigphQLcphTRphTR