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( 9>- $      &                                v          16E6R<D,."&  gP,,@II^^^{{{&&&999DDDXXXaakvv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Fcijjjiiihggeeca`_^]\ZWWTSQNNKIHFDCALMMEEEaaawww   ###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bbb11923d9b84cafbe2b6965aa091d7aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-Well%K1336_A2_Passiflora_terminiana_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zzzyxy  ?dBl=`{#Kj}w`OC8*}MjK, wS6 4EKG=426>FMT`v L2g@jp1Ro%^,wlhm|(8AC@@ESf{~vsttof[SQXh| X>_T3^PS|zthXG7*$4?A9-  &( yfP9% $-4871(  ,9I_|6cvI)rB3.G;=G<|C,Z7#!%(**)(''()*)'#  #%%    &(  &/2/)%%',/0//159=@AAAA?9/" !/8:6/(%&))&+De;yu8*xTtc>-&" #&$   !Y=`T]c:#   9uo@fF& $$      "! 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M7B (-PK>RG&B_>Q?[$ F [ D*A+X$*<L09U\$NEbBF8N9DG/9F GSB 95 GNVD7)]K!IE&/#TV)P!Tc9GU#, #<1C=3F@<01G^E GQ =1F^3%"7P@N<NR(82Z-&HYJ9[;$"V))^W2.QI1;6$ :-[(#C[>.D(J?)MF$<3+!3B*^. E4?2J`^Q"N]TD#E%_8-b@ .DOQ "U7!\5aW%!^#L\S4K*6.$#<g_eW?+,"OCXl/vQLLt<yC:YS&&aK7Gf<U+eL\z7?CW kvRm=) B/hmAKq)eTkJiK!M[s*0,-PpvbY<+gZRlI|) <V)@_#U 7|:\#&/dCDc2pH]'" [&^Gn}Mdq`gD/A6-?3  ]82 < ywPRildas_[0i_qY|7R6,T%unYb)@@ ,z3J6)${[X8J'"^/.   1  4 $5 Q)4c"R*2  !+'T)'%!Ms :CG%!$0g$ ,ppj,e K>AR.ERQLMv,e7HHtqVJ8A\0'FhRV7 &W;,:Lo S-,58\X:dr 1#dnFV3QMZou|3n_ML4u ufa}e;3TJ^PM"^a`lqT?!WsJ.RzrrUx@Sw|l<2qC\:f7s~uATC3K!C:f1!"@fOI{igZ6mqx`jnL[UUotWhI7$&Pak;2Fv%)d*Uf(b>i58`vt9-;~Dtlj+MT@W!/%bxqG&]:6rg< 1fe$!Lq}rl)5] m ^JdCACCGGCCATAGAGTCTACAGAATTAGTCATGGAATATCATTACCCCATTTGGCACACTGCATCTAACCCCAGAAAACCGACCTTCATGCACTTAATCGAACACGCCACCAACCCGGGAAGCCCCCCGAACAGGCATGGGCTACCATTAACCCTTAATCCTTTCCCGTTCTTTAGAACTTCAGTGCGGACCAGGGTCCTTTCCCACTTCCTTTCCTTTGAAGGTAGGTGCCTCGTTCTTTAACCTTCCTCCTTTCCGAAAAATCAACAGGTTGCCTCAGGCACCGTACCCTTAAACTCCTCTGCAACTGCCCCCGTTATATAAGCGCCTCTTTCCCTATTAAGAAGGTGAATTGGGTTAACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:37 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtIASPtIB1PtHB1PtHBCTSAVBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9sTDATA 9sȿDATA 9s<DATA 9sDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~?LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS$P1AMk3 P1RLk04P1WDk5P2AMk;P2BAkk>bP2RLk8PBASkk&GPBASkk$PCONkk.PCONkk-^PDMFTPDMF$PLOCk*PLOCk'PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%$SCAN@SMED SMLtSMPL&&TSPACAp>SPAC$SPACAp>SVER4.0SVER AKSVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH$phDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtIASPtIB1PtHB1PtHBCTSAVBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA 9sTDATA 9sȿDATA 9s<DATA 9sDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabOFVocOFWO_GATCFeat//OqGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~?LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS$P1AMk3 P1RLk04P1WDk5P2AMk;P2BAkk>bP2RLk8PBASkk&GPBASkk$PCONkk.PCONkk-^PDMFTPDMF$PLOCk*PLOCk'PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%$SCAN@SMED SMLtSMPL&&TSPACAp>SPAC$SPACAp>SVER4.0SVER AKSVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH$phDYbigphQLcphTRphTRFj6%n?oX?HYTw|bT|MI>"oCTRM*?Bcz6F&ZC'nUz}%?^`0L)^,KUS|Xq:u0#|UR7\6|y9o31)-PA)y1XV7:>JMB*u $O6T-jpjK4|<"G+SP7T]Zjp7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/&({T?HK_eXjKy#Id`Po<_+MWQ(SbXW,VE+,[Ol% eIe]/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGxNMVp:12(`Mo0Q0yA./GTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9A1=SUSm|b0BIGbH|'$cu\<]*:853`lEA_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_bP2RLk8PBASkk&GPBASkk$PCONkk.PCONkk-^PDMFTPDMF$PLOCk*PLOCk'PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0GRunN""JbS/N%$SCAN@SMED SMLtSMPL&&TSPACAp>SPAC$SPACAp>SVER4.0SVER AKSVER,,/Scal@Scan@TUBEA2Tmpr<UserphARphCH$phDYbigphQLcphTRphTR