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   $98IMiZXOK5;- 7,,@II^^^{{{&&&999DDDXXXaakvv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Fcijjjiiihggeeca`_^]\ZWWTSQNNKIHFDCALMMEEEaaawww   ###+++323::?AAGHHOOOUUU\\\bbbhhhmmmsssyyx}~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 74ddc922d1754ada8a8454f20bb5e54bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_15-52_08843130Seq16973135372_135372mfagan96-WellK1336_A1_Heliocarpus_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zLOQTUSMD<9>K]p~~sdYSU]elnmifegkqv{}}|ofditumiksukm|#7GNI8 {y!(2C]}4So0MtL}<h4Nlw3{#j8=r M %\}v,{ | . 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"10"_%DP* .F-H:  D (4WUPC$OCUM^=((^(_8C$IX+=F'TOU)a^T6$XI>WG ?ARV>b;c64J MWTEDaFM'/*6CU1>aYH<UA1N 65O,aH9 K+.S)2P'2.H;7CXQSUH X. Q c'#QBHRS%BGHbV$@JbS/+>NI- 'H?J)@XE*&S'56(a^P + T!$B8A%5AS/JR 9bP6"FSF>%_.K#4ZYXCHTb=dM C;7&NLbbKE)U4('J!A=::,:R^a>; cG/c'C I@!#6>  4BU$ @+Z?S7XQ`_]?(*N?NG1/TAQ/M'13JZJAY %V VI 4:A=PKVR0/ cW89 Q')S)c1 M[5>0:cAHPG*W _=H%= 17a!=N&Q Z R1RJcA|X: " !P &-f $|x @__Y|9tmPE i7)el*OL.W87AKYx!gKZkUxx9keXQI |V8O} ''klfaf:,v'u t^-\II;3}Z}%CUoJ~Y<(ko#mt8FR1`R][,J4YI,thLJ*XMQlIBVnU'=.M); eMkfdmSB_-*34N# q!Cln ;#Bpry??}V>-' Y{iu D?Q:yb$ 6*8#-]Oo{8.xbvO  ~AF|0Y39*)H6,nHv!T1Qr<m`9xxyMBmz {w$ V0T \zbN~q[hhrF&rR^mH =6tBc N'TN+A?+QS0():~_D"8?(& !,%1   & +$  .#$D)  *        " ;54!H (   (7  L%, -1  o-<:": /J -!m  2 !" O;3%B(/_X -+ M9 /)$(V[-R/i--T&  1!iH3$$8 -55$[5C+3--7%Wh;>>-6L5(((-2--5--lY="zlipZ@(8DI78CVpa0qX"~854+D<Wel['dse5lQs!'\O*&dT, :97 +oO(y=A,"y/@M?*pVPYGd#d8y#ZltITHsH>8LQEU\wWPN8%85,$,,Pc`K+- .8LuB!JtRE4\DN2%855I4c'cfd3&d''_e_yO18_nX}\nl RBq+,d"0J&u'<B}8BsWZ5Q71 B{5)s.a" m+2V9"hU#;7]C}5BxFdTC7>oRjwG.Nwjs(]W@{,3T?Y.@sJ^~(iN!#bs[T7:]VXSdsX^KW2%CAT[X6747S,(f6QrQit_oS|0~IYGkK;x_;nBc{WVVDP~Q{obpV.BgO_WBf1:J=N:\EKIJ=1Ew28ubBk:}77Qc`\h#JWX]/F(XwbUB Z|yRr<~VnLl%5O}sCTHThzVRZlXFX<?}PVYMMqE8RsmbFkm?P9D]T5,a*NG[`_V;S|xtGsrTas`d7`E/Gf&u`6U.u-f>^lG*pE_^Rc~8hir3VsIj rin@!x2H.}wY=.=A 1OLpQ^m~1g{!#K=Ki46 i CACACTTTTTGTGATTTTGGTGGAAAAATATAGAATATCAGTACCAGATTTGCATGACCATACCTAACCCGAAAACACCGACCTAAAAGCACCCCACCGGGCCAACCTTGAACCCGGGAACCCGCCAGAAGAAGGTTGGACCTCCGTCAACACGTTTTCCTTTTCCGGTCACTAGAAAGGCCTAGTGGCCCGCGGCCCTTACCCCTTTCGTTATTTGGAACGGAGGCTGCATGCTAATGACACTTGCCTCGGTGGGTAAAAATGAATAATTAGCTACCGTAGCTATCTCCTTACAATCCTCTGCAGGGGCTTGAGTTACAAACCAGTATCTTTCCATGGTAAGAACTTAGCTTGGGTTTAAATCCCGGCACGCTGCACTTCAAGTGGGAAGATTAGAATCTGCAGGGTAAGATGAAACTTGCTTAGGACTTTTTTATTTTAGACTAAGAAACAGACATTAATCCTACCAGGGAGGCGGGACCCTTGACTTCGGATTCAGAAGACCATTAAGTCGGGTATTATCCGTTACGACCAACGTTGGAGCTGTAAATGAGCGTAGGTGCGGGTGACGAGAGAAATACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 17:39:36 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}1ARTNASPFASPtASPtB1PtB1PtBCTSEBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA DATA UDATA &)DATA 8jpTDATA 8jpŶDATA 8jp6DATA 8jp^DCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0)EVNT 05EVNT0AEVNT0TFWO_GATCGTyp!!0sHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~CLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:OfScP1AME/P1RLE*P1WDE3P2AME<P2BAEEAGP2RLE83PBASEEPBASEEmPCONEE(PPCONEE& PDMFTPDMFQPLOCE!PLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNBRMdV1RMdXBRPrNJrRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0gRunN""JS/N%2SCAN@SMED SMLtSMPLTSPACAoSPACJSPACAoSVER4.0SVER E SVER,,/SatdScal@Scan@TUBEA1Tmpr<UserphARphCHhphDYbigphQLcphTRphTR&This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}1ARTNASPFASPtASPtB1PtB1PtBCTSEBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA DATA UDATA &)DATA 8jpTDATA 8jpŶDATA 8jp6DATA 8jp^DCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0)EVNT 05EVNT0AEVNT0TFTabSpFVocS_FWO_GATCFeat//S0GTyp!!0sHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~CLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:OfScP1AME/P1RLE*P1WDE3P2AME<P2BAEEAGP2RLE83PBASEEPBASEEmPCONEE(PPCONEE& PDMFTPDMFQPLOCE!PLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNBRMdV1RMdXBRPrNJrRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0gRunN""JS/N%2SCAN@SMED SMLtSMPLTSPACAoSPACJSPACAoSVER4.0SVER E SVER,,/SatdScal@Scan@TUBEA1Tmpr<UserphARphCHhphDYbigphQLcphTRphTR]VX(p:-"UZ~en?R/FIOb]_C} IBEBW8w|J"Wl&8{[ Mb.$ru$IC6~bTUV5PZGf$ZP}Gs+OStx0Rx;i,sWYT8FBj'~;Hk@*w&RAXWl>jA:>y7$?js3W4KuYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEefwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]ML.Me+bS_?Z p~!*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f7k7:J&q~}JIXEG%w8.2_/ KRZHlEDx8_1tm9A1=SS<}#gll0jZS[H#WSW>7.c$<\U["iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NHUV$tU)(iA>oOyqIQ7}:EBu&h`%Bf73ibT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^iAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}1ARTNASPFASPtASPtAUDTcCB1PtB1PtBCTSEBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA DATA UDATA &)DATA 8jpTDATA 8jpŶDATA 8jp6DATA 8jp^DCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0)EVNT 05EVNT0AEVNT0TFTabSpFVocS_FWO_GATCFeat//S0GTyp!!0sHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~CLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:OfScP1AME/P1RLE*P1WDE3P2AME<P2BAEEAGP2RLE83PBASEEPBASEEmPCONEE(PPCONEE& PDMFTPDMFQPLOCE!PLOCEPSZE`PTYPJPXLBRGNmJRMXV1RMdNBRMdV1RMdXBRPrNJrRPrV1RUND RUND RUND RUND RUNT 4RUNT 'RUNT RUNT &Rate 0gRunN""JS/N%2SCAN@SMED SMLtSMPLTSPACAoSPACJSPACAoSVER4.0SVER E SVER,,/SatdScal@Scan@TUBEA1Tmpr<UserphARphCHhphDYbigphQLcphTRphTR