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   ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a75de3020a8e4a678d03df031409ac23Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-Well"K1336_F6_Bomarea_acutifolia_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z<<=?DLVcq *OxTX7JlD '   !Ekl3v-PT?'2!vKAx"l%dRS3 ? . 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(."8% A#  2&))U!*6*.*7<$)8:1-'()2A  '/"0 2/679CW;EUM73@M  "       ppppp).@18^Plf6 MLZiKppcem3m>wd1F,R3cA7U+m F6 epv9,fcpF<Iz{B0El7e1|hC FGpYwX{Cz%zfEJp}_p'KEpKMYjr*{\k; Hxr_[ V) 8T7a$!ipO[1'|Yg*#Zl\LOa+M dkupnZ8+YAwQmO*H)p7C]{p<F*7pppCZ8pkephO6M \ + V 5 /^+/q +  ;-X Y!8M-hl8B%E<c;'($1"1$+-es[/S2%,=* 8-.$4-!"9!66("h 6 .!%)'%' 2D)##1/"$#%+"! 1. '08"7%5$-#(#% $&,+()!  $".,##.#%F< * )# !'$#$ 85% !!, #%)%!(! +1(,5 * $5 G-5&#"!33;/G""5<'E6%1#+4'"2#1.9332jG$-)%9VWd5-2U""*)A'B4!3"+5F@K> &*WLM=17SEZF:0#-%A(  $%9& <&SL'8bU-V01)(;+-T% 8;B;u712J&1I5Y:]0Qxc4Hgq]rNGGGGGTGGGGTTTTTTAAAAGTGATGATAACACCTAGTCACATAATATTCTGCCCGATAAGGCAATCGATACTCCCCCCTCCTTGGCGCCCTTCGGAAGACTTCTCCAACCCGAACTTCTTACTGAGGAACCAACGCCGCCAGAACCTGCCTATCCTCCTCATGCTTCCTGACTAATGTGTAGGCAGGATGACCCTTCCATCCCTATCCACACCGAAGGAGAAGCTTTTTTCATGAGACCCGTACCCTAGAAGGGCAACCCGATAAAGGGGAAGGAGCATATTCCAGAGATCCCACTGGAGTAAACTTCCATACCCATCAGGATTCCTTTCCCGAAAAGGAAGTAGGTTGGGATCGAGTTCCTCGATGTCTTAGTCTAGAATATATGAGTAATTTTTGTGAATAATAGGGGAAGATTCTCAGTTTTCTTTTTTGTGTTCGCGGTTGTAGTGATGTAGCCTTCGAGGGCTGGCGTTTTCCCCCCCGGCGGATGAGGTTTTCCTGACCCTTAATGTTCTCCTAGCAACCCTGTTTTGAAGCCTGTCTGAATGTTACCCCCAGGGGATGGAATAATCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:52:18 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt*ASPt*B1Pt)B1Pt)BCTSRVBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA :)tRTDATA :)tRDATA :)tR=.DATA :)tRDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~PLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS%P1AM@<P1RL@8 P1WD@A P2AM@J P2BA@@NP2RL@EPBAS@@(MPBAS@@& PCON@@5PCON@@3PDMFTjPDMF%PLOC@/ PLOC@*PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%%SCAN@SMED SMLtSMPL##TGSPACAl/SPAC%SPACAl/SVER4.0SVER RKSVER,,/Scal@Scan@TUBEF6Tmpr<UserphARphCH&phDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt*ASPt*B1Pt)B1Pt)BCTSRVBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA :)tRTDATA :)tRDATA :)tR=.DATA :)tRDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab`FVoc`FWO_GATCFeat//`qGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~PLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS%P1AM@<P1RL@8 P1WD@A P2AM@J P2BA@@NP2RL@EPBAS@@(MPBAS@@& PCON@@5PCON@@3PDMFTjPDMF%PLOC@/ PLOC@*PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%%SCAN@SMED SMLtSMPL##TGSPACAl/SPAC%SPACAl/SVER4.0SVER RKSVER,,/Scal@Scan@TUBEF6Tmpr<UserphARphCH&phDYbigphQLcphTRphTRTFo@L15FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3{,89Lb_b.O_l;5)F}o-W2Bj9}x{:LkACj_-2)O~P@CLD&/{>SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(2#1-O$>9@_diUo[Ojl}|/_'yBu&h`%^fK3cbT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m*0_fs$X=<;#(eaK|`!t/[z~+7K>0Et'b'v6R> uVswJW%R7R>(hZ$~I!|spN$t.Dd^4G^({C!RJ<6Q^Zew.k.d}uNf1&vM\#YTEl?&az?>FN uc&/S,o~(Is;AVq&8aw31K}i>"IBN.,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS