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   ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bfcadb93e65d42fbbf9b04ba63dc521eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-WellK1336_F2_Peperomia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z$<Tn:oGr*ut^ 2  m  4 c    c 0 s , W % } A 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"""                   v@_A_AL.IAKB.bcpKB_3730_POP7_BDTv3.mobtermTAGAGTCGTGATCATGCTCGGTGTTAAAGATTACAAATTAACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCACAACCAGGGGTTCCCCCCGAGGAAGCAGGGGCTGCAGTAGCTGCCGAATCCTCTACTGGTACATGGACGACCGTATGGACTGACGGACTTACTAGCCTTGATCGTTACAAAGGGCGGTGCTACCACATTGAGCCTGTTGCTGGGGAAGAAAATCAATATATTTGCTATGTAGATTATCCTTTAGACCTTTTTGAAGAAGGTTCCGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGCTTCAAAGCCCTACGAGCTTTACGTCTGGAAGATCTACGAATTCCTCCTGCTTATTCCAAAACTTTCCAAGGCCCACCTCATGGAATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCTCTATTGGGCTGTACTATTAAACCAAAGTTGGGGTTATCGGCTAAGAACTACGGTAGAGCGGTTTATGAATGTCTCCGCGGTGGACTTGATTTTACCAAAAATAGAGTCGTGATCATGCTCGGTGTTAAAGATTACAAATTAACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCACAACCAGGGGTTCCCCCCGAGGAAGCAGGGGCTGCAGTAGCTGCCGAATCCTCTACTGGTACATGGACGACCGTATGGACTGACGGACTTACTAGCCTTGATCGTTACAAAGGGCGGTGCTACCACATTGAGCCTGTTGCTGGGGAAGAAAATCAATATATTTGCTATGTAGATTATCCTTTAGACCTTTTTGAAGAAGGTTCCGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGCTTCAAAGCCCTACGAGCTTTACGTCTGGAAGATCTACGAATTCCTCCTGCTTATTCCAAAACTTTCCAAGGCCCACCTCATGGAATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCTCTATTGGGCTGTACTATTAAACCAAAGTTGGGGTTATCGGCTAAGAACTACGGTAGAGCGGTTTATGAATGTCTCCGCGGTGGACTTGATTTTACCAAAAA /:IUp| )6CNXcky &1=KValx%/;EQ]jw$/9FR]hu%1=ISanz &0;GR^kv !-:GR]it'5ALZdq}    ) 4 @ M X c p z    # . 9 E Q ^ k v    ( 6 B N Z g r     & 1 < G S ^ j v    ( 6 B M X d q }  %0<HT_lw)6BOZer~&3>JUbo{ $/<ITany !-9EP]jv*7BLXdp| #/<HT_lx !,9EQ\hs)5AMYfr&3>JWbnz $/;GTamy !.:FR_kw +8DQ\iu )6 /:IUp| )6CNXcky &1=KValx%/;EQ]jw$/9FR]hu%1=ISanz &0;GR^kv !-:GR]it'5ALZdq}    ) 4 @ M X c p z    # . 9 E Q ^ k v    ( 6 B N Z g r     & 1 < G S ^ j v    ( 6 B M X d q }  %0<HT_lw)6BOZer~&3>JUbo{ $/<ITany !-9EP]jv*7BLXdp| #/<HT_lx !,9EQ\hs)5AMYfr&3>JWbnz $/;GTamy !.:FR_kw +8DQ\iu )6   :5 (" *66-06:>6>>/8888++8>>/',2,>>;>>;,>>>;.>;>>;>>;>>>>;;;>>>>>>>>>66>;;>>>8>;;>;;>6>>>>>>;;;8.;>>!>2;;3>>>31;0"'1>>>>>>;2>;;>;8>;>;;>>>>>;>>;;;1;;;;33;>;;+".""8;1;;8;.;(183;;;3833238+333>;2;;;>81.83133;;>0+2+;22818;811;3';313321118.$.1312.+3.  33;>>>>">>>>>>>>>>>2>>>>;>>>>>>>6;>;;>>>>>>>>>>>>>>>>>88;28;.>>>>>;;62;;>>>>>>>>>8;->;>>>>>;;>>>>>>>;;;>>;>3>>>>>>>>>>>>>;>>>;;;;>>>>>>>;/>>>8;- &8>>>>8>>>>>>>>>18>>>>>>>>>>>3>>>86*828>>>>>>>>>8>8>>>>>>>>6>>>>6>8>8>>>>>>>>>88888>>8>/->/>+8'.'>888(&40>1>>>6>>..//+440/    :5 (" *66-06:>6>>/8888++8>>/',2,>>;>>;,>>>;.>;>>;>>;>>>>;;;>>>>>>>>>66>;;>>>8>;;>;;>6>>>>>>;;;8.;>>!>2;;3>>>31;0"'1>>>>>>;2>;;>;8>;>;;>>>>>;>>;;;1;;;;33;>;;+".""8;1;;8;.;(183;;;3833238+333>;2;;;>81.83133;;>0+2+;22818;811;3';313321118.$.1312.+3.  33;>>>>">>>>>>>>>>>2>>>>;>>>>>>>6;>;;>>>>>>>>>>>>>>>>>88;28;.>>>>>;;62;;>>>>>>>>>8;->;>>>>>;;>>>>>>>;;;>>;>3>>>>>>>>>>>>>;>>>;;;;>>>>>>>;/>>>8;- &8>>>>8>>>>>>>>>18>>>>>>>>>>>3>>>86*828>>>>>>>>>8>8>>>>>>>>6>>>>6>8>8>>>>>>>>>88888>>8>/->/>+8'.'>888(&40>1>>>6>>..//+440/ RR$&,!"$REGL% >/RO O$A# :I_P*B0?U!L8"-3XC+R]1 =Z Z"MEE.?W8 0ID)41]0-'I D7W-JE!B5FU0+0b &@T>L?X^BKW,.[SUM +6FU^U`&7SI/A4UQ]:\%VHd ;YZN'T$S#Z2S%EJR T Y N*UB AVDT@?<,T  c1OPYFH.]-0% 2M[G;,K#T+<R8!A/-3_Q&P8<77=cHUH4;S\ MQ319.^,L;"'d7 2TP=c7W`X55^3"RR736U4PT`XVGXYA bB$ Y,Y^O;7%U%L]P1Q W L`b^=17ECK <&HT7N,B[H3KT8YHIaaX4KB9Z +S44b,"] Vb?6BQa7 T<Xb'-b XJK[3F>%bB8YF0<F2$QKN\"7&N"# .V42K6 7O;Td,BcO E"%DO HF Y":<9@;&3PID2V2  *YCc sKC6Za~F1 V}ycaIIB09 5KV|c%"#01d-'*DEM fM@)R?JZI@a<x6VCT JCIq~wT Wppn[8,2Q/ r}l?[6e&9_H F38b)ilKFA$9dRV_;x/^Fe P\:$zmO;Br(j mrxbYig2>?a6U#1"D3ng}=4;a <o!<dS]qIeonxrT?Zx<C9cPEM{ p"(i5|O[.$#O7l5-lZD`e*;<ap;?n;m~ 3/ FyU|n:`B@P ~E_v]VT^]BDJC@@1+=,;7?&>?($#6    ,&()      uL      ( +   0 bY   4%  - "*( (    \01  1*1& .#3?+d8>I,(E28 "#X1G!_*> Ev!  >L@h,<ICj+Zp^A*sgdpbn}l`GUYNUS9#17d8x K&6$BTM3\B''L,d7{:A pl/-.5PC`LY;3C2rc%#>u&_S 8fTvKlb4aLA7UPfgt2d-RtD?JNW  Pw?R5pd'YEpp?LH84X^_,pp,eIPVLnu q,rA!Dpp8n;)dp:@  ytH@R a7bB3E<nUN^ &%:A0J:?( $=tvXxd%F?CJS*)?L;-$])&  ']nM((""d`%I ""3#s%#T* $p T %vs)(t,;r(q#&Y*! 0OkYKZ<s`q-VjYD.UO^gzxW$3E[~vf&|i2t*/{m[-lhlMQ`jrH``*&!AMxy.!'8)5(4> ,,($5EV; Cb'1" !)50,5210&:&,-*E#,*!/#/,N1&f#'%e<+('$#70(#B/#i8G/3Gi=:GKFG$6#8,Csuh*++0/NZ5?';<Yh;45RO\.BK6?[):A 0"%.0('/1.A\Kd"bV/%#G`YL*A\CR,!%F-7%- /YG!T1#*!-AC.P~K&.<1k>EHp+F54&?M>o-{?}7M=?-!@,6le9SE(wt lu(IrxGGTGTGTTGTTATTAAAAAAAGTGATGGATAACACCTAATGTCATAATATACGGCAGAATAAGGCAACGGATATACACCCCTCGTAGCCGTACTAAGAATCACTGGTCCAACCCCTGCTTGTTAAGAAGGATCACACGCATGAATATCCGGACTATTCTCTACATGTTCAATTTAGTAACCCTCTACTGGTCTGAACTATTAACCCGAATCGGGGGTTATGAAATAACAACTCGGCGAGGGCGATTAATAATGACCTTCGCGGTGGACGTGATATAGCCAATCTTCCGGAGTTCCCCCCGGAGGAAGGGTCCATTCTGGGAAGACCTCCTTTAGTGACAATGAAGGACATTAAATTCGAATGCGTCTCGGCCCTACTAATGGAGATATTAGGACTTCTACAGAAGGATTCGGCTAACTTCCAATGTTTTCTTCTTTGGTTTGAGAATGGAGAGATTTACTGCACGAAGGAAGGAGTTCTAGCGCCCACCGTAAGAAGTCTACCGAAGTCTAGGTGGCCTCCGTTCACTTCCCGTTAGACGCCTAAGTAAGTATTCTCCGCTGTGGAGAGAATAATTCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:51:48 -04:00AEPt@AEPt@APFN T{APXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTS9BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 6mTDATA 6mtDATA 6m0dDATA 6mTDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~7xLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS LP1AMC#P1RLCP1WDC'P2AMC0P2BACC55P2RLC,)PBASCCPBASCC PCONCCTPCONCCPDMFTdPDMF cPLOCCPLOCCPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N% DSCAN@SMED SMLtSMPLTGSPACAqSPAC \SPACAqSVER4.0SVER 8SVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCH zphDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN T{APXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTS9BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 6mTDATA 6mtDATA 6m0dDATA 6mTDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabG\FVocGKFWO_GATCFeat//GGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~7xLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS LP1AMC#P1RLCP1WDC'P2AMC0P2BACC55P2RLC,)PBASCCPBASCC PCONCCTPCONCCPDMFTdPDMF cPLOCCPLOCCPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N% DSCAN@SMED SMLtSMPLTGSPACAqSPAC \SPACAqSVER4.0SVER 8SVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCH zphDYbigphQLcphTRphTRFB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TVc:q?tKb"Z\F!+= A~K2p$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*rZW*WEX|~4m!m!@lhP0Br\ NV#JX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0t[XAn@xg-;!ig-g0wMrH88K7zaKz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAa^{?nNL)H"i=Q<,{AmXXt;*hZyP4)@Dx`575^@!&4?ZCKwdJ][pGCj?}:EBu&h`%^fK39bT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^iAEPt@AEPt@APFN T{APXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_AUDTUoB1Pt^B1Pt^BCTS9BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 6mTDATA 6mtDATA 6m0dDATA 6mTDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabG\FVocGKFWO_GATCFeat//GGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~7xLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS LP1AMC#P1RLCP1WDC'P2AMC0P2BACC55P2RLC,)PBASCCPBASCC PCONCCTPCONCCPDMFTdPDMF cPLOCCPLOCCPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N% DSCAN@SMED SMLtSMPLTGSPACAqSPAC \SPACAqSVER4.0SVER 8SVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCH zphDYbigphQLcphTRphTR