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   ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 902df99a2580451da5fccb60401c4b1cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-Well"K1336_F12_Monnina_ciliolata_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z\^agq})C]y*Y,bdG:b'Niz ""(:\ 9 l  4 Q f o l \ =  y 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O8'i*qyh9zylH4y9  6oIqtP uIHs"zc h2@/uh }?FXvnCn7   #R:{`bsWp!{? /gQF~nfj{B   *^Mby\n p%r7C0z+J#<p.'a7   $Q*jd!lm'g\N( =p#AC't? #NC\W5P- 5`jMMnVNg7  "L'ps/^?pG/$  $P;Wb1          #M CbeJy6apa8~M*    <k7C5CuZW/     5e#QgaB(^rBQ0  :hBVR8~[A/$     !   &M-NUCwL-   $.6::5.% 'Kz ##S/  (/330*#       %J}3XeV0Z9$  CyCvwH hI6-)&"%<`& `E3)%##%'),/245542.*$ !!#%')($ 6i"EK5Z0     #-=Uw+v Zr\QMJGC<4,% !+4;?AA@=81)        q@AU2AUeAȴKB.bcpKB_3730_POP7_BDTv3.mobtermTCGGCGTTGCTGTCATGGATGGTGTAAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAAGAAGCGGGGGCCGCAGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGTATGGACCGACGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACCACATCGAGCCTGTTGCTGGAGAAGAAAATCAATTTATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGAAATGTTTTTGGGTTCAAGGCCCTGCGTGCTCTACGTTTGGAGGATTTGCGAATCCCCCCTGCCTATACTAAAACGTTCCAAGGTCCGCCTCACGGTATCCAAGTTGAGAGAGACAAATTGAACAAGTATGGCCGTCCTCTATTGGGATGTACCATTAAACCTAAATTGGGGTTATCCGCTAAGAATTACGGTAGAGCTGTTTATGAATGTCTTCGCGGTGGACTTGGATTTTACTACATCGGCGTTGCTGTCATGGATGGTGTAAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAAGAAGCGGGGGCCGCAGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGTATGGACCGACGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACCACATCGAGCCTGTTGCTGGAGAAGAAAATCAATTTATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGAAATGTTTTTGGGTTCAAGGCCCTGCGTGCTCTACGTTTGGAGGATTTGCGAATCCCCCCTGCCTATACTAAAACGTTCCAAGGTCCGCCTCACGGTATCCAAGTTGAGAGAGACAAATTGAACAAGTATGGCCGTCCTCTATTGGGATGTACCATTAAACCTAAATTGGGGTTATCCGCTAAGAATTACGGTAGAGCTGTTTATGAATGTCTTCGCGGTGGACTTGGATTTTACTACA09>Kgny.9GR`jz,7BPZep|)3?IUam{ (4>JValy "-8CO\hv+6ALYfr~)6BOZep} "/>HS_kw  " / < I U ` j v    * 4 @ L X d r |  ! - ; F S _ k w    ) 4 > J U a m y    + 8 D O Z g s ~ '2=JU`mx)6BOZeq~%2=JVbn{ "/;HT`lx+7CO\ht&1>HU`mx ,9EP[gs'4?LVamy!-9ER^jv(3?LWco{ #.:GS_lw+6CO[gs~ %2>IUakt}09>Kgny.9GR`jz,7BPZep|)3?IUam{ (4>JValy "-8CO\hv+6ALYfr~)6BOZep} "/>HS_kw  " / < I U ` j v    * 4 @ L X d r |  ! - ; F S _ k w    ) 4 > J U a m y    + 8 D O Z g s ~ '2=JU`mx)6BOZeq~%2=JVbn{ "/;HT`lx+7CO\ht&1>HU`mx ,9EP[gs'4?LVamy!-9ER^jv(3?LWco{ #.:GS_lw+6CO[gs~ %2>IUakt}    ' #6:4.::&/>/6//>>>>>>>>>,18>,>>>>>;,>>>;,>;>>!1>;>>>>>>>>>>>>>>>>;;>>;>>6>>>>>>>>>6;>>>>>>>>>;>;;>>>>>>>>>>>>>;;>>>>>>>>;>>>>>>6>;>>>>>>>>>>;>>>>>;;>>>>;>>>;>>>>>>>>>>>>;>>>>;>>>>>>>>>>>>;;>>>>>>>>>>>>>>>;>>>>>>>>>>>>;;>>61>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>8>8>>>>8>>>>>>>>>>>>>>>88&8883 &8>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>4>>>>>>>>>>>>>>>>>> $4 4,,44:6646:6:..::::6616666446646":61::6!4%2%6./012235-1:21:.4522442*6+1+ .     ' #6:4.::&/>/6//>>>>>>>>>,18>,>>>>>;,>>>;,>;>>!1>;>>>>>>>>>>>>>>>>;;>>;>>6>>>>>>>>>6;>>>>>>>>>;>;;>>>>>>>>>>>>>;;>>>>>>>>;>>>>>>6>;>>>>>>>>>>;>>>>>;;>>>>;>>>;>>>>>>>>>>>>;>>>>;>>>>>>>>>>>>;;>>>>>>>>>>>>>>>;>>>>>>>>>>>>;;>>61>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>8>8>>>>8>>>>>>>>>>>>>>>88&8883 &8>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>4>>>>>>>>>>>>>>>>>> $4 4,,44:6646:6:..::::6616666446646":61::6!4%2%6./012235-1:21:.4522442*6+1+ . WJ *R#MP=I/U[.$@PD.+R"a=a<>9 >[FY66 FUOUIA06 =" Q2?J> 0^8U$>1TZCI ICAH_UbRHcE,?"7IUK HBYS:d_BF[a5/3(%6<>XJ>UWZYXY,` +#1b:TA#E??<:LSAC" FGa8)7?c)3U"B!AB 6:`BK-c$M=Y P!@#9%98;-c.KY5>'Q I/\+9.Hc';aO[$$W'Y70V !3.CPR<?O^Y/b%XE*!'J/#$ T2Z^T]89\a1&56-2K `$07\<+)#6OG $7]+ab6*CN-OH^T?V S%FJP3>W?'K+,#2$C3&PJ")L?bX 9 [1._16/ "3(X<483]c:@BG&&Z5!T-#R\IEBB?;_DI/UV"I]HCd-O%U>/N:6>9G**!O"3 ;T _9b&**5H>,@A+/4!b0$9 .N$cM!N/CA bB-_P325\-  Z,8X2=2Sa#W  hEO5XOlsb%@=t.ACLIpX}PU;:2yY|eE@p\\L"M`D@8Dy$"W)/;m0pGW&LdI}J'L|AA;"mU]?0)}v}SR^O *Tx/ux2]PEx4S7{RK%w(Bi!\nFf.KV;G6n;Jgl<RQA@lb`J^,=8rm$&IZo}gk]z3(BtB QM7R U>'#z-c W6F}6fhn ye9+l,~_ H&UcT{"+!-ybDGhKWz~z8JUy9B0ijB:wq$n :w9R,S +J!|su`_1V@Q9zdUfVITYJOO@DH8D6-Q\4J.1" +4-$'!#                    "2# ) 3(!)0" 3'+ -# #+!8#.), !*+9,/ + 1'J=%*2689P//4K<?*?==9($b.h5%7)'4A1UIPASD=>FOaCHG2CH:++66)+++++)Hp6pQ,nF8p $Up\W  ,V Fd,Z#~vpu0dpv8d?e:~Q,jR#0p+ 8'1d;,91>G]8s,8Kh1T;x`W8805h[Y69%83\/d#&8Zf}F&x $K! ;ih!$;3*8gvRChk7c84TpDkt{([,Z)jak*p8$MS?d}8 ,) y8hw # B {Z,p)4HENx %yQ0faW@?/LQVF*(ID&Jg"'7bX5'9$  '  " G?!!'% ! +@ "%# $  & + /". $"(# &@ *""2&-  ( 4%(@ ")!2%$ $#$!!"!-,"!'3))$0 !  %"+ + & *"!#,,-1!#68?9S?N3l#8a0 '<32"0)A9z*@ '34&"(%**(")3 #*J4 '1N.>',J)"##1s{$$5-T3L( *'SKRJdaU`P@U25+aG/F91D2`f]Uqiz01O_['zD1Lkh[/G1AAAAGCGGTGGTCACGAACGATGTATCGATAATATCTAGACACATAATATACTGGCGGATAAGGCAACCGATATACCCCTATAAACGCCGTACTGCGAGTCAATTCACCATCAAGAACTTCTTAAGGAGGAGACCACGTCAGAAGAGCCGCCGTATCCTCCAAAAGCACCCAGACTTCTGGAACGAGCGGCCTTCCTTTCAATCATGATCCAGACGGAAGAAGAATCTTTTACATCGCGGCCGGTGCCAGAGGAGGGTATCGAAATAATGGAGAAGGAGCATATTTCCGAGTTCCACAAGGAGGAAGGGCTGATTCGGAGAAGAATTCATTCAGTGAAAGGCAAGAAGGTTAGGTCGAAGTTCCGAGATATCTTAAAGTAGAAGAAATGAGGATTTTTTCCTTACATTATGCCTAGATTCGAAGTTTTTATTTTTAGATTCGAGGTGAGAGCCTCCTACAGGTCGAAGAACGGCATTCTCTGGTTCGGAGTTGCGGCGTTTACCTAAACTTGGGGTTATCCGCTCAACAAAACAGAAAACCAGATGTAAATGGTCTCCGCAGTGGTAGAAAATAATACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:52:55 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtuASPtuB1PttB1PttBCTS!4BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 4hTDATA 4hDATA 4h$DATA 4hDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB YP1RLBP1WDBP2AMBP2BABBiP2RLBaPBASBBPBASBBPCONBBPCONBBQPDMFTjPDMFPLOCBPLOCBIPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%SCAN@SMED SMLtSMPL##TGSPACAu[SPACSPACAu[SVER4.0SVER !)SVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR=This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtuASPtuB1PttB1PttBCTS!4BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 4hTDATA 4hDATA 4h$DATA 4hDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab/FVoc/~FWO_GATCFeat///OGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB YP1RLBP1WDBP2AMBP2BABBiP2RLBaPBASBBPBASBBPCONBBPCONBBQPDMFTjPDMFPLOCBPLOCBIPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%SCAN@SMED SMLtSMPL##TGSPACAu[SPACSPACAu[SVER4.0SVER !)SVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR.iTr8p|r_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*8ml%RZnKnO_1o!(7q<[Hc:=Gk;0|5Q1V?|&@8ja:3AGXS<< xrTW?iMAkc PkIp?H^Poa#]}Ko)6V8JX|K2-!;9xxsWD@|DmOY,f *"49J_'JKxwQ4fC\{lKd#8 UC;2i$@jEl0bU)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JUx2ffU"&6,2SayX,8JThnbi2dP?l~Wo]yi-IX/TI}Fs8G> zNpQ6p+'vQ%jXW[ly] 2,\auB[*mK#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL