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   ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f8d8ade65c9f4630bb307ad9cb968f1dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-WellK1336_E2_Roldana_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z|+NvBnkn!M-  u  Z  M | s \ C *  d =     q @  v #umb d|W:$ 1Lg~iR:  (*' 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Eg Yd \fCm&K }`:F`a6ql2~Gy=F-l"qg(O|dvD$/l;6<u*N()@y&(F3Ep}U{<jM,#{gQCuw:ub:hd|f=XMh48@'/ocie? lW _}aJ"=(Sv >a1IOGNByZ'01d &z.1:N$SG7 0m><_Ro(\XC7Fdv#t~ ;fr"2  %YRhv{~= cja] \9r/\LZ Y$|$SgKX\^;U<P =q0dREwSa|[L2!(OWp!67Z8^M:N/ ,>n d ')X?Iu-3 *"J<tJLKk`bPGeD,LCR=8((1DU 1#4/' &             0 *       " (&*2= +!% 2. )#/4$" 1"1 : 'G"26"3*,,'( /x!(3c,O2".*C?> P !3H!/2:)=Tptp} *jnYMp17]/Z;h\O~Fpk,2|4\GN^80OjVrp.Ip8~}>gId#7IS8ZZ0E~e zroNdH \}&5-(dKIp%,jzOYUjgBwd+ ofF%KaT)xRq;]%EE?r$7dl!, dK F2;p b~kphIcUb0MM|`V%z&4];<~3wrW!f!eNU 1}") |   LRcF;" ]1 li E&,Qf=/YP5P98(2+3J\L''O}' + $4coX%^"- *' #4-'5" (4"2 6 #9$E1"L(#-?%#!:5$$<)#$!!B *!'%+$+N:8*6!.")!+P!/*I": (% !1"'!*2%)BQ&$C!8.7&/#8-"#4'""+' /&.,G)&+*-1%'12.8G$ *"1+%B'5,35(%/%O3%D"5A17/&;ACU ;'77d$2%P*,0K-',G*A %[^<;B6.)@J#3+) 0O6!$~4*=,@Keuo*!(oQ<7J`5$(8)##v3$0?'0 V/'A2NoC>$ Fr749,hsd%($ 1B[.%%;'(1/8#K'QGP#?W4P%89-E'.o%-%NJT%q(MF_N:z0-;E_<tc%kT2M[mvM9^\CEs AAGATGGTGTGGTAATTGAAAATGTGATGATAATATCTACACACATAATATACAGCCGTATACGGCAACGATTACACCCCCCTCAATGTAGTAACTCGAAGTAATTCACCAAGCCGTGCTTCTGAAGTAGGAACAAAAGTCGCTAGTTCTTAAGGAACCTCTAAACGTTCAAACGCTGAAGTGCCCGGCGGTTTAACCACCATACACGGTCGCCACGGAAAGTGAATGGGAATTATCCAGGCCGGTTGCAGAGGAGGCAAACGAACTAGCTGCCGTGGCGCCCGGGCCACCTTTCCCCCCGGAGGAAGGGGCGATTACCTCACACCCTCCTTCCGAAGAAATGGAGGAAGTAAGGTCCCAAATCCGCACGATCTTTCGCAACCAGAAAATAGTATTTCCACCTCCCGCGACCCTTACCTTCGATGTTTTTATTTTTTGATTCGTAGTGCGCCTTATGGAAATCTTGAAGGCAGGTGTTTTCCCCCCCTATCCAGGAATCTGAACGCGAAATTGATATTCTATGGTCAACTCCATAGAAGAGCAGATTGATAAAGTCCTCGTTGTGGAGAAAATCATTCCCAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:51:50 -04:00AEPt@AEPt@APFN TyAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTS@+BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 7oTDATA 7o*DATA 7o3DATA 7ozDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~>LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS*P1AMG*#P1RLG%P1WDG.P2AMG7P2BAGG<[P2RLG3?PBASGGPBASGG]PCONGG#NPCONGG!PDMFTbPDMFAPLOCGyPLOCGPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%"SCAN@SMED SMLtSMPLTGSPACApjSPAC:SPACApjSVER4.0SVER @ SVER,,/Scal@Scan@TUBEE2Tmpr<UserphARphCHXphDYbigphQLcphTRphTR<This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TyAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTS@+BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 7oTDATA 7o*DATA 7o3DATA 7ozDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabNFVocNuFWO_GATCFeat//NFGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~>LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS*P1AMG*#P1RLG%P1WDG.P2AMG7P2BAGG<[P2RLG3?PBASGGPBASGG]PCONGG#NPCONGG!PDMFTbPDMFAPLOCGyPLOCGPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%"SCAN@SMED SMLtSMPLTGSPACApjSPAC:SPACApjSVER4.0SVER @ SVER,,/Scal@Scan@TUBEE2Tmpr<UserphARphCHXphDYbigphQLcphTRphTR,9drWdG| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz9n#m+mI?p7 RVi}d!-v6D5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! K]T5^.?s|"Ff61}XTu.ycI0 r#U4-BnFc:&YnKh9Zin5&OkG>:U$N+Yy;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6M/N:RVNg#7LvgU7[-TJ`"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(IwGCOLw~^|ToH?+RX,CF 9H[p"6:P!gFS]T'_C:rF(5,f *"LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS*P1AMG*#P1RLG%P1WDG.P2AMG7P2BAGG<[P2RLG3?PBASGGPBASGG]PCONGG#NPCONGG!PDMFTbPDMFAPLOCGyPLOCGPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%"SCAN@SMED SMLtSMPLTGSPACApjSPAC:SPACApjSVER4.0SVER @ SVER,,/Scal@Scan@TUBEE2Tmpr<UserphARphCHXphDYbigphQLcphTRphTR