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(-@biWb\{`\j[fuu`aRVRduw}PN%5%CUswruS:&66EEB4 1+.' #    !>L7D?:$  <E,,,III^^^{{{&&/99DDDXXXaaavvv     ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRUdhkiiiihggfedbb`_]\[XXUTQPNLKIGECAMMM)EE^aawww    ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ebcdd685957c4c52b1863f933643782eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-Well K1336_D8_Urtica_spirealis_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zOQTZbkrx~/GcK|*hY Cv'W~eM8(%+3=LbxI6/9]jR@4-+0<Oi$T'V~mP5lZF/{S- }tolkkjigda^\ZXVTRQPPQRQPNMKKLMOPPQSW^hs*jSM6B8Q'q-{ogcbbbbfqHls}?svKTM $JqqAff;}^PdSe[@/'&'*++)&#!$3MwZjUSaE3*&'(*+*)'&$"   2bL/RV:OX) V+ei@|?    !Um )4B      8w?iq*Gr0    8! {4    $AkyL*   !WZ  6uX{wO x@ #S[!^"   ,v]3 7X&TkE& :q>_\6b, 8cT,  .DZkspbM5  +C[nup`H. 0I`orhR7  ;VkvseO7$*8BHF?3% !0>HKG>1$->KRPF6%  '.21,# )141)      #$!  !   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"     &        "      "("" ""   "       #$"     32'1%)"'4:4.66/6*/8//>'>>>>>>;>;;;>>>>>11>>>;+>;;>!&6;;>>>>>>>>>>>>>>>>>>>;>>8>;>>>>>>8**18>>6166668;6;>>;>>88886!  "     &        "      "("" ""   "       #$"   ]O`,X[=F*3=-KZ;31='b SM9 L!$] .->@34 Z =1/:Q'\6N<< ."U T:D[M/3"0P\:&Q 0L*F=\;.OM! 2SQ;4S WY* #9I: = QEaZ5'N^*YW+P9=bJL[bFBVB!22%! =%N#_P >4c:\0*_KSQ&d/S01F/RWSA" 0@\[CF)Q,(%NY b'M8+-XG&S K Q78Cd1OK/*,R,5b"L"V  '=%V(NM*A A2K &c9I[bcM6E,X%4:>T??Aa7$d a`Q2L8.%"Y\.*5@Y$X!T,`?Z  ,Q !5sy&."J1z&t]zkeLD;ln.CA5P%Z!0j2ltzv/`S6s%s:f] 8 }y?:*,F W9`o9MA`4$~*mAgOWZ$ -#u]0KZXR}KCn$44 O{I\09v5+N &.kdy8<\U^kRn (xEvP>.*kV Z ^KZ[>/g83+G9LH }>3s;r\;BnYgXX9LHX>=R@A;-.6+Ni.C0>D*%'&3( $+    5+>.4p% z"h08T 4 0  B _U8Kd!B!-B!t-*9(1!"Y15e) T" U01'Jppt1p@zdG ~a~G,AY|2cGZ?0R84pi3E4Jt h,{+ }uU)?n5WcArP~}z5!ip#"A:G,-[j )d\kh5(I~'i,(rZQ,Wx,OX: K T\-HgH7#c9*tn] \z|dV%74 --5C~' ?^M!#K,651{8T;.4-* "#,(Z<":):%'0#i(&:<I6P8A:?<nm@Ioiy GI7 9kb\u7SA0+QHIek6<kQ$Ee~!B +}=Ix*8]Mb_UMGX23M  $+p(i]6R'!}\;}CF"Aiz.UHe~zAAAAAGTGTATACACCAGGACGTGATGATAATATTTACACTAATAATACTCTTCAGTATAAGGCAACCGATACACCCCCCTAAGAGCCGTACCTCGAATCAATTCACCAACCAGAGCTTTTTCAGCATGAACAAATTCTTCTATAATTGCACGAAACTCATTTTGTTTCTATATTTCTGTGCTGAAAGTCTATCCCCCCAAAAATTATCTCTACCTTTATTTATACCTGTTCCCTTAGAAACCGTCACCGTTCTTTCTCCGAGTCACGCACCTATGGACTATTCGCCTTTCCCTAATTGCTGAAAGGTCTCAGGCATCTCCACTCCATTCCTAACGCTTACACACATTAAGACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:52:37 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtEASPtEB1PtDB1PtDBCTS,BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 7nnTDATA 7nndDATA 7nn2@DATA 7nnDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~*yLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMbP1RLbCP1WDb P2AMb&SP2BAbb)P2RLb#PBASbbPBASbb3PCONbbPCONbbPDMFThPDMFPLOCbPLOCbPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%SCAN@SMED SMLtSMPL!!TGSPACArSPACSPACArSVER4.0SVER +SVER,,/Scal@Scan@TUBED8Tmpr<UserphARphCH.phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtEASPtEB1PtDB1PtDBCTS,BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 7nnTDATA 7nndDATA 7nn2@DATA 7nnDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab:\FVoc:KFWO_GATCFeat..:GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~*yLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMbP1RLbCP1WDb P2AMb&SP2BAbb)P2RLb#PBASbbPBASbb3PCONbbPCONbbPDMFThPDMFPLOCbPLOCbPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%SCAN@SMED SMLtSMPL!!TGSPACArSPACSPACArSVER4.0SVER +SVER,,/Scal@Scan@TUBED8Tmpr<UserphARphCH.phDYbigphQLcphTRphTRK2OI*-V~>yj6m}:)9`[?y[nvbeK]TM7l:2Fj62o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbV_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKM\8}48vc. 7$?Z2o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*M0JNbm|XMrDK"*.GHiJ(/\_'MChMoG;KMJ95nM,aVh6dYHp-Op;ZJLgS]jTj.uFk4W7|!y]18`'|_n?26.?bFWJQ:Ocl2[ALYZBO"T@{:/tR(_i`jxFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WebtmWmcC$K"2OE;-A>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_^Z0b>"EJX.=DaJ2`>nX$t!TQqvLWHO$O Z>d{sb[!Pk}V PwHJmv[SN3(u<\8KZI\T(k"T'o)6yHAj}=