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   ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bd39684c71fd405e8d92ca31dfd03056Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-WellK1336_A1_Heliocarpus_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z88:;<=<;8655566531/./269;:61+('),/11/-+)*,.000.,)('(*,/010/-+****++++,-/111/,)'&&'())))(''(*,/2579::;<===;841.--/010/-+**+-...-+**+-/257998742/-+)('''(*+-.0147:>ADGJMPSVY[]`dhmqtttstw{:X" I BekI)m*H X T r L 8 7 F ` y ~ Y  n _ dhe cjpDZn-k '+M%x4G~6ki1i/~$ee>'Dg_dq__n>8~khU6$ !"#$$%$$#"!#,<Tt=T_]M0{S5"%7Pr,TnwmR(Z5 :j5tYL$  I@d l7 %Fpb:  8`f<  2Uc: .hkx$s4   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TVATAATTTGTGAGGGGGTTTTTAAAAAAATGATGAGTATATTTAGACACATAATATACGGGAGTACAAGGACACGGATACTCCCCCCTAAACAGCGTCCTTAGAATCACTTCACCAGGATGACCTTCGAAAGAAGGAAGAAGATTCGCTAGAACCGAAGTATCCTCGGCAAGCGGGAAGACTTGCGTGACGAGCGACTTGCCCAACATTCGTGAGCGCCAGGGAAAGCTATGCTTTTTCATCGAGTGCCGTGCTAGAGGATGGCATCGGGAGATATGGTATGGAGCCCTTTTCAGAGTTCAAAAAGGAGGAAGGGCCGACAATGGTAAGAACACCTTTAGAGAAAATTGTAGGCGTAAGAACCCATTTGCGCGCTCACGTTCTAGAGAAGATGCACGGATTTCTTGAGGAGAGAATGGTAAAGTACCACTTTTTTATTATTTTATTGAAGGTGTGAGATATTTAATTGACGAGGGCTGGCGGTTTAGACCCCCACATACCGGGGGTGGGGGGGTCCAGATATACTCTGAGCGATGCACTATAGAACCGTCACTGAGTGTAACCCCCATAGAAGATAACAAACACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:51:56 -04:00AEPt@AEPt@APFN TAPXV2APrN 1*APrV1APrX}}13ARTNASPFASPtASPtB1PtB1PtBCTSIOBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA &eDATA 8qUDATA 8qDATA 8q8DATA 8qvDCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0YEVNT 0eEVNT0qEVNT0FWO_GATCGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~GLIMS!!0LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISNOfSc@P1AMG3GP1RLG.P1WDG7P2AMG@P2BAGGEP2RLGThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 1*APrV1APrX}}13ARTNASPFASPtASPtB1PtB1PtBCTSIOBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA &eDATA 8qUDATA 8qDATA 8q8DATA 8qvDCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0YEVNT 0eEVNT0qEVNT0FTabWFVocWFWO_GATCFeat//WjGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~GLIMS!!0LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISNOfSc@P1AMG3GP1RLG.P1WDG7P2AMG@P2BAGGEP2RLG{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{MKz\\ZqqkN{O78r<,d;ui|6R&^aC.?bFWJQ:OcPJ~FTWYI!8U{~DkO/t457fc-,RIEWR9HmGPle9Su35-T|%(4Ws _ZZ^kq"CsINi@C43ofR5q5lef5U_9}_Pt;LiP""#]eq }X'"7NI=smc'Kx3} 6hf!Y '(doz1)U`]yVoJ@GNPSXr6Yu]fc]__O.Fin5&OkG>:U$O+7y;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6M/N:RVNg#7LvgU7e-wN}"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(IwGC1"wv_|QJX?*V2isW/&~5T.N5#r[FIxLqRJ4ir$GG\}d MfaqQl&A6PG>}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt@AEPt@APFN TAPXV2APrN 1*APrV1APrX}}13ARTNASPFASPtASPtAUDTg}B1PtB1PtBCTSIOBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA &eDATA 8qUDATA 8qDATA 8q8DATA 8qvDCHTDSamDySN 0Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0YEVNT 0eEVNT0qEVNT0FTabWFVocWFWO_GATCFeat//WjGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~GLIMS!!0LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISNOfSc@P1AMG3GP1RLG.P1WDG7P2AMG@P2BAGGEP2RLG