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'  7PvYQ<,  "I,7<IcxFTX_VV Zimo]IV9-   $00HDLG4?6*$ A<E,,,III^^^{{{&&/99DDDXXXaaavvv     ***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRUdhkiiiihggfedbb`_]\[XXUTQPNLKIGECAMMM)EE^aawww    ##*++223:::AAAHHGOOOUUU\[[baahhhnmmsssyyy}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 16e690ae471b48caa5da04661da21dbdSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-29_14-05_08833130Seq16972135369_135369mfagan96-Well*K1336_A12_Cymbispatha_commelinoides_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z[\^bhouwurmjikpv|'@^,eY+b7.9 1  z O  M z u c S D 2  T $  n C  C?`2{&`5&9JZl197,kJ/ jG oG#~{yvroligd`\YWX[_cdc`][[]aflt~V@cXfTV6)3S<(6=(u)~ujkwn7DFK%Bh8ed3\$`c;"    0Kog5XbS.t.|R3     <{Oc:hb)J?s6         \]G*_90]E h h2  =x!{GN    TvDEyX     )ze WaD[T^+  RZWLI9|j!}?Ct>dM D  +[DG%= ATC, E\]|>E=^ a' &[Bvi&lg7^-B  ,R [$*x{Mc6Tk&  8k&xA1Zo. 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G2<SFIM#bACQE_S %$ V!*#RcaU 1d1^GZI-5N ;<LK""5/FD\AHT["DHWXc(IC6L 7D (1\B#A \ ^FKL-.1>F82E&%U]I C> ;, D2Q=."Q3+X5)! QBP:('(@7$]TI\;1V7SDK3d6L 1]*4`9-<S*&] ?AFC["X-9`H 10IWN&49Y5.*JAJ?\d4WE`)T4W% WLN,RGRS\\Y$:N8 Qa41@='#9(a*[! &,BGR[Z6*2R- "?TT:/H.$,4MP7 4FXTI?O)$Q4X.=[C [4WP;ES?D #6..VR+0HYT]4!?N,N$C^'=A?-2@a*GA*-F[!-^ MI8#B_264Q @P %_!&XCSG>:0"%Q:9=b/@0\#'R)5@d 16&A"N 5(/Q2 'B/N;X=[c? ,! jIb:q%:V ,D'/(2,Xx 8vfk3_j>y7E~&+^ &YpU3w Q0 0@M dVa7LxK]NcbdOJ5/g23\C Mtg-Dc^jy3:cC.yYJN6iV,>n} Qh'a`QBZEVpkP"y<t/r:S1/4>4O}"N|i 8*Pm= (%e;0oC5c E[C!V&ApIlS9 tQk?[Liv4oK  {BsK>C Tto` R ([kT}f'k_ oVG{E,A]m>eZ_:2-Uj)J%QV>n]"HX V>3;E.yxh;h S|;vWG4x + [ ~ak)DB]rh cWrYGM\5B\:=D92./I/6!.-))'+&                      & ' ,35&##  &?".*5"%!'/(2 )3"&5021:E)-$AH&F<I/N&BDG"#I=*-?5)?;ABV7)44 %%44 %% % % K1zpgppppp{8P\p|0dB8djp=+8YM"W{mTs!WiepC-*6- LpQzP(zKprR"@ w4 8pBU8yD$l%V1R#o5ddpV[%h*>B!Kro 7: ke|[hLV=p%z`CYBGCOJd Zh;(f`8c1p@n.8Q 0X "SMzg>vH!+9'@9VD + "+' /%7'(^X!9[65-DB248W018<h;mMH3iQC=(4W>Z>r/M[ ; WGTGAGAGGGGGTTTAAATAAAAATGTGATGATAATATTTAGACACATAATATACTGCAGAACAACCCTATTGATACACCCCCATAAACGCTACACTGCGAATTACTACACCGACATGAGCTTCTAAAGAATGAGCAATTTCCGCCAGATCCGCAGTATCCTCTTCATACACAAAGACTACTGTGCACACAGATTTTCCCTAGAATCCAGAGCGACACGGAAGGAGAAGCTATTACATAGAGTGCGATTCTAAAGGAGGGAGACTAGATAATGAACATAGAGCATATTCAGGATTTCCAACCGGAGGACGGGCCGATTCTGATAAGAACTCCTGCAGTGGAAGTTAAGGCGGTAAAATCCGTGTTCCTAGATCTCTTTGAATAGAAGAAACGAGGAATTGCAATTACGAAAACCTCTGGAGTGGCCTTTTCTATTTTTGGGCTCGAAGTGTCAGCGATGAGCAGGACGAGGGCCGGAATTTTACCCATCGGCATGAGAATTTATACCTAAATTGGGTATATTCGCAGCGACGTACGTTAGGTCCGTCAGTGAATGTTTTCCCTATGGGTAGAGTACAATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-29 15:53:04 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS 7BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 3gTDATA 3gLDATA 3g$DATA 3gDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMC SP1RLCP1WDCP2AMCP2BACCkP2RLC_PBASCCPBASCCPCONCCPCONCCGPDMFTrPDMFPLOCCPLOCC;PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%zSCAN@SMED SMLtSMPL++TGSPACAw|LSPACSPACAw|LSVER4.0SVER  ,SVER,,/Scal@Scan@TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTR>This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS 7BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 3gTDATA 3gLDATA 3g$DATA 3gDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab.FVoc.FWO_GATCFeat//.RGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMC SP1RLCP1WDCP2AMCP2BACCkP2RLC_PBASCCPBASCCPCONCCPCONCCGPDMFTrPDMFPLOCCPLOCC;PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%zSCAN@SMED SMLtSMPL++TGSPACAw|LSPACSPACAw|LSVER4.0SVER  ,SVER,,/Scal@Scan@TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTR-'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWi`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{nQ&y#H9E :}r!@xk^+O} 6(5D~VCKUf<+Nibymn]z@4)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9]CR"lWW .C&q6X+9muy%lFLuLGtrwE5[W{V=zb9+b{M_37hVo+&k=;A{%i|o(>V&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBArxPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_M%_|_n?\h =&e=1&!rE_g4YDI#%+AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT<B1PtB1PtBCTS 7BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 3gTDATA 3gLDATA 3g$DATA 3gDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab.FVoc.FWO_GATCFeat//.RGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMC SP1RLCP1WDCP2AMCP2BACCkP2RLC_PBASCCPBASCCPCONCCPCONCCGPDMFTrPDMFPLOCCPLOCC;PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT RUNT 3$RUNT RUNT 2/Rate 0RunN""J2S/N%zSCAN@SMED SMLtSMPL++TGSPACAw|LSPACSPACAw|LSVER4.0SVER  ,SVER,,/Scal@Scan@TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTR