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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 16eacef863404fc1ab05acf2b3c32596Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"############### K1322_H4_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z#.Cc8Vv9Zy9Yq}{utz~eN8"j\VVTL>*veN2lWB, $1C]%Fe9JV_egbR8}vpi^L3vZ;re[VTSRQQRTVXXVSPNNNNMJGCA@BFKPTVVTRPPQTWZ]_`bekvBtMzP'tB}zz} E7R` 9awzkM OSW^4JB| 9uZKC=6.$!"!!"$&&%!",<Pi$f;JC&o&yW@/" #$#         ! 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K]MM!5 "\K`A+C c$5@E8 LY$"&S*`6T. TmzQkt$ryU E$ LsDYR!?|-0~x)%Mu%.r('85b:wtUJel$0+C4B3(+fA2*c\'K0] crU5x$G3(6.1EyXU/!"G1ZIs6N,Dm |b@Sm I"=_ZT>#RK\Jzx 9F6#MY7U&fvfU : hjd|UO}{U8+4p05EX9[/OS c(oZyYDF89fcYb 3`3?\+VbOhE#3LS9W+Q>@XjN0V.?$A>AqpV4 9Yj  A\1s1(O  *[ #|!0h? v?s@a?8{)yjumeHZklCV_LN6&",3B[4J#B;- E<(!5(                       1  % '&Q    %!/.. !7 (1 (-$$ "5(,(#8$'/$$!),2>!0D#)2+-,1,CBT7,3;665H94= :$RCF)/M!!//!!!!!!! !![!pppppmppg~tpnp%] X,rpqK azUw~a]w],pe*VDgrWru}vY: fq+Hdp})pTy+4/pkNwJV'OJ_Xw-s+>O Dv{v+1U; Q cd]8[pQEYM,6z&E$?}kmuKT} \eto/sp4e 8\EcdL:]\PlfAbp{p8cx|/Ftq9$33|P, b]u%j5WN}G" %Q)FI4?BeN^=5q8:-4% %1"#(-5($(#!:#*VE1 $8 $"!0#%f-7(DG)% 2.""'"1%, "7[5.E"2-* ",$&5)51)( 7&0-(E/6%/ ,82$,'$K" ;37:>Z%>9L&R#_!%-&)7:,4#% .5 $9+(*@.I.8:0/2*U<00(:$"!,1 &!(*&;7d=.=0-? +) #6() +'8;.#,#+35S*!20&+63LBq+&0F0<=*66I"< +(0*+  $@#A2b*+1?@]nP#&#)-%!" 0N %%)9"!%-+(5M;C^5(0:38 FZ-&9,%+ # %@\h+("3D& gs0/7"+FN:CM@wI,VJ&@9\}kpQE?J<7s5YxKL= Z1*E<ZCGGATGGGGTTTAACAAGTTGTGATGATAATATCTAGACACATAATACGGGGCCGTACAACCCAACGAATACACCTATATCGCTGCCACCCTTCGAAGTTCTTCTCCTGGCAAAACTTCGTCAGGAGGAAGAAAAGCCGCAAGAGCCTCATTATCCTCCGGTTGTACGAAGACTGCTGGGAAGGGAGGTAGACACATCCCTCGTGATCGCGACGGAAGGAGATTCCATCTCCTCGCGGGCGGTGGTAAAGGACACAAACGATATATGTAAGATACATAACCTTCAAGAGATCTCAAAGGAGAATGCTCTGATAACGATGTGAACTCCTCTAGTGCAAAGGGTGTCGGTTGAATCTGCTTTCCTCGCTCTCTTCGGGAAGAATATATAAGTACTTCTGGTTCAGAAACGCTTTCCATACGCCGTTCTTATTGATGTATTCGTGGAGGGAAATATTTGCAGCACGAGTGCTGTCCTTCCAGGGCCATATGTTAGAACCCAACCGTAGCAATAATATCCGCCCGGTAACTCTGGAGAGTGTGATAAGTGTGTCCTCTCTGTAGTCGTATAATAACAATAGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:21:27 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTSDlBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 8apTDATA 8ap|DATA 8ap6>DATA 8apDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB.P1RLB* P1WDB3P2AMB<P2BABB@P2RLB7PBASBB?PBASBBPCONBB'PCONBB%PDMFTPDMFPLOCB!PLOCBPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPL!!TySPACAgSPACSPACAgSVER4.0SVER DaSVER,,/Scal@Scan@TUBEH4Tmpr<UserphARphCHphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTSDlBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 8apTDATA 8ap|DATA 8ap6>DATA 8apDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabRFVocRFWO_GATCFeat//RGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB.P1RLB* P1WDB3P2AMB<P2BABB@P2RLB7PBASBB?PBASBBPCONBB'PCONBB%PDMFTPDMFPLOCB!PLOCBPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPL!!TySPACAgSPACSPACAgSVER4.0SVER DaSVER,,/Scal@Scan@TUBEH4Tmpr<UserphARphCHphDYbigphQLcphTRphTRvTLYr*XV=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+%3NA0B98n~*q\{l_]De_Wg&TiY;S=AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKST/4f_7}?e_MBSmv^N6T.iUlt.R;kHh1T6'U"ziVZ?:'(r;4QV/fcd]R03^jQJM~j3XV]'DZNuOUf?eKkE=+4[PngD??Vc;6,A;{IF}oIiM%.zP{?7.N{C]*KWeq }X'(H;E}&mxXYnuPQh^*w% e MfaqQl'[)VBL$7V5*o&w+\kf*>Pyr{;qe&Ker"&O93s'!byH:/ULLwvhQZb{./bT(o]7}WcoSVTkx&(Mlw:'4xe:^*!t p7_lnqR9JK[Ylid?O>JWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0x9zG@}d>4"AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt AUDT`B1Pt B1Pt BCTSDlBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 8apTDATA 8ap|DATA 8ap6>DATA 8apDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabRFVocRFWO_GATCFeat//RGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMB.P1RLB* P1WDB3P2AMB<P2BABB@P2RLB7PBASBB?PBASBBPCONBB'PCONBB%PDMFTPDMFPLOCB!PLOCBPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPL!!TySPACAgSPACSPACAgSVER4.0SVER DaSVER,,/Scal@Scan@TUBEH4Tmpr<UserphARphCHphDYbigphQLcphTRphTR