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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: aa11f9173eb64c3c8be119ff82d2f125Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"###############K1322_F10_Stevia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z 8Xw,C]x !-8?BA?=@FP[ekorx"?Whsvtng`WJ8Wn8zS. %Kw(Nr:\s{wj[PHA7#s_J5! 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G @@AMAKeAɆKB.bcpKB_3730_POP7_BDTv3.mobtermAGGGTCTTGGGATCAAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGAATTGAGCCTGTTCCTGGAGAAGACAATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCTACTGCGTATGTTAAAACTTTCGACGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGATTGGAATTTTAATCAGCAGGGTCTTGGGATCAAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGAATTGAGCCTGTTCCTGGAGAAGACAATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCTACTGCGTATGTTAAAACTTTCGACGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGATTGGAATTTTAATCAGC"+:Eaepw+8DQ^o{"-9GQ\gt  +6@LZfu~!-7COZer%0<HUao} $/:EP]iu ,9EQ\ht &5?JUanz   ' 1 > J U ` m y  ! + 7 C O \ g t    ' 4 @ K X d p }  , 7 D O Z f r }   % 2 > I T ` l w  !,7DO[gr~ $0=IT_lx+7CO[ht)5BNZfr~%1=IVbny !-:EP\ht*7BNYeq~&2>KU`lx -9ER]ju)5ANYeq}'2>JWcp{ $/;HT`ly+7BMYcoz&"+:Eaepw+8DQ^o{"-9GQ\gt  +6@LZfu~!-7COZer%0<HUao} $/:EP]iu ,9EQ\ht &5?JUanz   ' 1 > J U ` m y  ! + 7 C O \ g t    ' 4 @ K X d p }  , 7 D O Z f r }   % 2 > I T ` l w  !,7DO[gr~ $0=IT_lx+7CO[ht)5BNZfr~%1=IVbny !-:EP\ht*7BNYeq~&2>KU`lx -9ER]ju)5ANYeq}'2>JWcp{ $/;HT`ly+7BMYcoz&  ,3),$ &24:4.61)*>/>6/>>>>>>>>>/>8>,>>>>>;'>>>;+>;>>;>;;>>>>;>>>>>>>>>>>>6>>;>>>>>>>>>>>>6;>>>>6>>;;;>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>6>;>>>>>>>>>>;>>>>>;;>>>>;>>>>>>>;;>>>>;>>>>>>;;>>>>>>>>>>>>>>>>6>>;>>>>>>>>>>>>>>>>>>>>>>;66>;;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>3>>>>>>>>>>1+>>;>>>>>>>>>>>>>>>>>>>>>>>>>>3>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66;;>>>>>>>>>>>>>>;>>>>>>>>>>1>>>>>>>>>>>>>8828888838>>>>>>3>>>>>>>>>>>1>>>>>>>>>>>>>>>>>.>>>>>>.>>>>>>>>1>>>>>>>::::::::::6::::::::::6:666*:61:::::1::/-106:0:13:5:::.:312!)"   "   ,3),$ &24:4.61)*>/>6/>>>>>>>>>/>8>,>>>>>;'>>>;+>;>>;>;;>>>>;>>>>>>>>>>>>6>>;>>>>>>>>>>>>6;>>>>6>>;;;>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>6>;>>>>>>>>>>;>>>>>;;>>>>;>>>>>>>;;>>>>;>>>>>>;;>>>>>>>>>>>>>>>>6>>;>>>>>>>>>>>>>>>>>>>>>>;66>;;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>3>>>>>>>>>>1+>>;>>>>>>>>>>>>>>>>>>>>>>>>>>3>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66;;>>>>>>>>>>>>>>;>>>>>>>>>>1>>>>>>>>>>>>>8828888838>>>>>>3>>>>>>>>>>>1>>>>>>>>>>>>>>>>>.>>>>>>.>>>>>>>>1>>>>>>>::::::::::6::::::::::6:666*:61:::::1::/-106:0:13:5:::.:312!)"   " 6V46K"P07 50OOB 5+\=G*+Ab:<FXWHY, N.G &LK c)+WSZI [L`S5 .-X>>E^F),FREcP]&AA72#`^,5W?49/64RH\\b0 ")!;>18] ? H_F(PKDMCJ S<H$F=XXN,!G-68 /O!]M&? &.AY#, *; aJ.QL"[!^ ,%=':,6&@'2L ^8!N"\V4&H8DY 'a^8dJJ39?I$,HI+07=J<:U/KTMR 9(\& `XU'JOE?SLL0=P)L 5MV%QQabN3=OY0EaN #9.&J`KK)HS]W( J#)&$U32J538;SI\) *@CT R5) G/ "Za .\'=[$:<M),+>>,K.^E>2*H. ^7?] .` ]QS@!DD_:cG"&b1-#C3)?EU--WUG@R  A0()'6*db4@4c,"SK&*8@:Q7&= ;F^$U2A#[ @<[@L( >&BfsPzn:=u/c[YC~sb=z-AY PEX7]M0"5j,Y 8bWS<eN &gv"si@Y"lj/.nPsW\ LV > (P$JIJv E  ( R|tRP*<sskv +smkh7ZP$A_sd q2 }zjr,X|1  2Tr?Z36*'vYJG$ jtU^[ 0[<u0ynv4t|S ZE59rcvUl8y$2(4!F1cI#`|.rl'l|V--RBiV o~'#-0~rK}EE  :A;r?=kioI;/#B@  9k@ Y0_r# &#56i\t[YxWOMN5;ELG-G75306+?/)).  !   !  "          " ! #< '2%   -)!#-#4$),+619+/,*5&(0 G/(hC0N6,/*&53#;%" ;"/8&&&&#### #gK"pp1p,(X_p674evuUdmS, !Cm(Cj/-V$N;~4+uY"es(p 5*1v,\\TPw} cdn?p6ip^.`h%>K<S0-b?5U%=BJmd$b67q8M$*Gds8o "pC+lyd5x_ F:Cp;dmpA9WfLS8Ap|M/ upp{ C FZPh]p,K2W[ SS8V`p{cN8ApA|B8jp:pZ] hG~ [U8Cq?'ZN 6lU!4Y"dW9C* 0 3=Q8 EX#L)%& "$#%) #+*'D$C9)/0%#'"%+)!$#-%2)7  &' ,#6&#"&("!*+(%#1"$*,!#)"!41+7)'+/)& %&%?"*4+"=&'.$-8,$)+$B0%"!'C#'-$0.2$ (/,-!$&#(,,3%&2$(+''2.%*%'#-;1$&3 1*4")! -7T@imL.,&!74&%%1';:%$"#+),)! --,6EEM8&!#"3R' #()(':.!&6I>C$T,0?0$/!559t.#ZSH,^7u5-%^&16rKHK:`85P PlhT(SlpGAAAGGGGTTTTCTCGCGGTTGTGATGATAATATGTACCCACATAATATTCTGCCGTATACGGCAACGAATACGAATATATCGTTGCCGTAAAGCGAGGGACTTCTCCATGCAGTGCTGCGTAAGGAGGAAGAAACTGCGGAAGATCCGGCGGATCCTCGGGTCGTACGAAGGCGTATGGGACGGGCGGTTGAACCGCGATTCGTGATCAACACGGAAGGCGAATCGATAGTAACGAGTCCGGTTGCAGAGGAGACCAACGAGATAGCTACGAAAGCGCCGTTTCGAGAGGTCCAAAAGGAGGAAGGTCCAATTCCGTGGAAAACTCCTTTGGAGGAAGGGGAGGGCGTAAGATCGGCGTTCCGAACTCTCTTCCTGAAGGAGAAAATAGGATTTCCTGCTAAGGAAAGGCCTAAATCGTCAGTTTTTATTTCAGGATTGGAAGAGCGCGTTATGGAAAGCTGGAGGGCCCTCCTTTTGCGGCAAGAACCTAGAAGGCTTAGGTAGATTTAATATTTTATGCGTTACTCTAGAGAAGAACATGAGTACGACGGCCCCCAAGCGTAGTAAAATCAATAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:22:16 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSgfBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA <yTDATA <y6DATA <yGDATA <y:DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:P1AMBQP1RLBMP1WDBVP2AMB_P2BABBcP2RLBZPBASBB=9PBASBB:PCONBBJPCONBBHPDMFTPDMF:PLOCBCPLOCB?{PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%:SCAN@SMED SMLtSMPLTySPACAi?*SPAC:SPACAi?*SVER4.0SVER g[SVER,,/Scal@Scan@TUBEF10Tmpr<UserphARphCH:phDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSgfBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA <yTDATA <y6DATA <yGDATA <y:DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabuFVocuFWO_GATCFeat//uGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:P1AMBQP1RLBMP1WDBVP2AMB_P2BABBcP2RLBZPBASBB=9PBASBB:PCONBBJPCONBBHPDMFTPDMF:PLOCBCPLOCB?{PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%:SCAN@SMED SMLtSMPLTySPACAi?*SPAC:SPACAi?*SVER4.0SVER g[SVER,,/Scal@Scan@TUBEF10Tmpr<UserphARphCH:phDYbigphQLcphTRphTRmkYbCK)Ul_pD8Jq,./b _.uKxh6O1w^{l{iED>{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{MKz\\bqqhN{$Y8(<,A;Ui|6R&^aC.?bFWJQ:OcPJ~FTWYI!8U{~DkO/t457fc-,RIEWR9HmGPle9Su35-T|%(4Ws _ZZ^kq"CsINi@C43ofR5q5lef5U_9}_Pt;LiP""#]eq }X'"7NI=smc'Kx3} 6hf!Y '(doz1)U`]yVoJ@GRAS[rDnHmc'9_QpEX D%"ECY`{hys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;Lil_[A( Z((nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/Ygn