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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 47c85fee9ab647e0a31381c2104fe0e2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"###############"K1322_E10_Salix_microphylla_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zCazFv=u)f#bK{ '<LVZVNB4$yDpJeRIFEB?=>CKRWWSKA4%U%d.]: .Hf 3Wu  mZG4#{]@#ynd[RKFEEGHIHECAACFIKJGB?<<=>?@BELT`m~ >s?f$Y<{5aywXk"E{(xrnlns{Fj=6FA)H )GbsvhEl]A%jcB?KkI6,'%$%&()*+*)&$!#+2896.$1Kl.i  z?eF2& "&((&#       #%&$  #WRmOFHzG.#         =+)d,G  $(,..+&     +m_GEcx4     +Gy)Xx=jV-!'.3540*! 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WUq]l+*j\sY^$j"d}f&bxS>1)"       %R/td\7     $UNUtF*  2h^>;$<(?gC,         +V6[_7H0JzU>0& +KkY ]A0&!          !DxaZjK6(   !>j"XzpHrP8'        "'*)&    2Z81eB,!  7dV!&u]?.&"$3RoDzj^RE7+!  $%$   -bBv{Jp8    !)16:<>@ABCBBBFRhVWTV xsneZN@3( )<SlydP?2)"     8R@&AIAP($A]KB.bcpKB_3730_POP7_BDTv3.mobtermGCAGGGTCTTGCATCATGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAGGGGCCGCGGTAGCTGCGGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGACGGGCTTACCAGTCTTGATCGTTATAAGGGCCGATGCTACGACATCGAGCCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTACCCCTTAGACCTTTTTGAGGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAAGCCCTACGCGCTCTACGTCTGGAGGATTTGCGAATTCCTGTTGCTTATGTTAAAACTTTTCAAGGCCCACCTCATGGTATCCAAGTTGAGAGAGATAAATTGAATAAGTATGGTCGCCCCCTATTGGGCTGTACTATTAAACCTAAATTGGGGTTATCCGCTAAGAATTACGGTAGAGCAGTTTATGAATGTCTACGCGGTGGACGTGGATATTTAATAAACAGCAGGGTCTTGCATCATGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAGGGGCCGCGGTAGCTGCGGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGACGGGCTTACCAGTCTTGATCGTTATAAGGGCCGATGCTACGACATCGAGCCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTACCCCTTAGACCTTTTTGAGGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAAGCCCTACGCGCTCTACGTCTGGAGGATTTGCGAATTCCTGTTGCTTATGTTAAAACTTTTCAAGGCCCACCTCATGGTATCCAAGTTGAGAGAGATAAATTGAATAAGTATGGTCGCCCCCTATTGGGCTGTACTATTAAACCTAAATTGGGGTTATCCGCTAAGAATTACGGTAGAGCAGTTTATGAATGTCTACGCGGTGGACGTGGATATTTAATAAACA&/:MTaqw,8EQ]kz+6AOYdo{&1=FR^kx$/:FR]hu'2=IVcp~ '1<GS_kw #/<IT_jw(7BLXdq|    ( 5 B N Y c o z  # . 9 F R _ j v    ( 6 A M Y f q ~   # / 9 E P \ g s ~    & 3 ? 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J U a m y  !-9EQ\hs %1>JUamz !.:EP]iv*6BO[ht'3@KXep| $0<HS_kv*7CNZfs(5@MWcn{ $0<IUamy ".:FR^jv !,7CP]iv+6CNZgs'3@KWcnw !)4  !6%  #%.:4*61)6-4/6>>>>>>>>#>8>)>>>>>/,>>>,,>>>>;>>;>>>>;>>>>>>>>>>>;;>>;>>>6>>;>>>>>;>>>>;6>>>;;616>>>>>>;>>>>>>>;>>>>>>>>;>>>>>>;>;>>>>>>>>>>;>>>>>>;>>>>6;>66>;>>>>>>>>;>;>>>>;>>>>>>>>>>>>>>>>>>>>>>8>>8>>;;>>>>>>>>>>>>;;>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>16>8>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>;>>;;>>>>>>>>>>11>>>>>>>>>>>>>>>>>>>>>>>>>>8>838&888>>>>>>>8>>>>>>>>>>>68>>>>>>>>>>>>.>>>>>>>>>>>.>>>>>>>>>>>>>>>>>::::::::6::::::::::::66:4:.::6:::6:64/:6::4::2::6:/:::::22 0%(.6      !6%  #%.:4*61)6-4/6>>>>>>>>#>8>)>>>>>/,>>>,,>>>>;>>;>>>>;>>>>>>>>>>>;;>>;>>>6>>;>>>>>;>>>>;6>>>;;616>>>>>>;>>>>>>>;>>>>>>>>;>>>>>>;>;>>>>>>>>>>;>>>>>>;>>>>6;>66>;>>>>>>>>;>;>>>>;>>>>>>>>>>>>>>>>>>>>>>8>>8>>;;>>>>>>>>>>>>;;>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>16>8>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>;>>;;>>>>>>>>>>11>>>>>>>>>>>>>>>>>>>>>>>>>>8>838&888>>>>>>>8>>>>>>>>>>>68>>>>>>>>>>>>.>>>>>>>>>>>.>>>>>>>>>>>>>>>>>::::::::6::::::::::::66:4:.::6:::6:64/:6::4::2::6:/:::::22 0%(.6    *\$H6T$U7I@^0@ b[=M7[a05 Q9Qbcd:;]:C2C<7!74PAEJ1 c0RY' \%2-$U<+ ':,a_[G:@)@C`=+U\HPS&LR !9 .$ XVN'O*E5?37107V`1 cV2c%`)A4B+! ;?<* Ua7@*J^ 4 W YRIa?41/+5(&'> -Vb'bNV$c4+_:] ++=\7&> ^3dGG12$6Q /2 !>b+[K_!OQM]P$Ha :QL=2/93 aa/TU; Ca4+&L4];]K\"JNZ'98 U$!c<5<>O6= P8%WXEKH_]#O c1O?1@P7>b9 N(]"2 @_ 3U!*BcY@ KPY&R#!^J(<0X ,a>*d0 !Q4F&0H%/]T [ F%M5] \%!N+4V 0a !A@_<EH! =C-&UX K^.@ ?YZAPG0JM\;LW:\G,2%[69$` DT7b2 I, ,!3b( A cVmo.$+ fi3-1|-~-:|k~eP5Mb]K4cDI`s=-Ka#F Q zY2I l 7h4 {]pS)cv#  capE}q?(;t?zH`WJd2AVJ@v)P]14y=Ki k&R:>Wfz'>C^_3Fon)"5%>'+W H.y\OJK)_ej`@B{>y:{N!j3>]Rk!>;g4"0@?W`v!+=1L<xum09~}MCTmO{b5Dm:3t4) Ng H-U -W `lVqWfx%gfLUY^DGG,C=FEA<1258$+ %  "                   $  (!=  $:(- *3$$1 %  $ )1"0-"6%*$+*A0;/%/M,) 3/5>G;X4;;> 1A?;X6C2e FP;4=)*F ? BO85.( ((. .   .. ....)pZpzYjHP*NNN1K[k8t9fp=\Y,pA;kHV!6xu'rm'%6p N d5W6X,,XD8 J7\D .tVad,w8S/ctHk}pNdXp h"_bcu M+,`JepEs~K&J)[R< 8;23&7a}^{?*dop*p-m8O&"/,{i-kDDTpgp)pjp-])d,c 8 ) I*\~Xxd!C?)ofIou&g3Dh]GYPH H+:7S]C!'P|!0<='3&!%-" (.+(5+3/ .6)L;/76% R;(H=8,1*$&6!+'3 4&5O$/:#"!%(#(&%!'11<- K ( 8&''.-3*C,*4 21'.4;!G<&0&I%0 A/Q .?%%<2%&543 &/$ !)#&-&+!'-,2, .*. <=3E/$=F% % %, "<#?1'#/ +3''%B*D1G<035'0'"1.3?g'#!'':',+=7/ +)"#1#%(   !==UJq$L3"$=2F% ?(80*++ %)',"'"8CHR@2,( &$&:.C ;48"*"6%3%DPC$7< :%(b2!@2$,?<M7/(605E/FC2SMK&Y'_"0tA[QolY0c#Db\AAGAATGTGGTGTATCGCGGTTGTGATGATAATATTTACACACATAATATACTGCCGTATAAGGCAACGGATAGGCCGATATCGTTGCCGTCCTTCGAGGTACTTCTCCATCAAGTGCTTCGGCAGAAGGAAGAAATGGCGCTAGATCCGCAGTATCCTCCAGTTGTGCGAAGACGTGTGTGTAGAGCGACCTACCCAACAATCGTGATCGATAGGAACGTCGAAGCCTCTACATCGCGGCCGGTGGCAGAGGAGGCCAACGAGAGAGCGACGAAGGCGCCCTTTTCAGATTTCCAAAAGAAGGAAGGTCCGATACCGAGATGCCTTCCTTCAATGAAAGGTAAGGGGGTTAGATCGGCTTTCCTCGCTCTCCTCGTGAAGAAGAAAAAAGGAATTCCGGTTCCGAAAAGGCTTCAACTCGCAGTTTTTATTGTAGGATTCGAGGTGCGCGATATGGCCCGGAGGAGGGTCGTCTTTTTGGAGCCAGGAATAAGAATGCATAGGTAGATTTGATATTCTATGAGTAACACCGGAGAAGACCATAAGCACGTCCACTCTGTATTTGTATATGAATCATTCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:22:18 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTSpBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ={TDATA ={~DATA ={L@DATA ={DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISCP1AMEZP1RLEV'P1WDE_;P2AMEhOP2BAEElP2RLEcPBASEEFDPBASEECPCONEESPCONEEQPDMFTPDMFCPLOCEMPLOCEHPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%CSCAN@SMED SMLtSMPL##TySPACAhSPACCSPACAhSVER4.0SVER pSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHCphDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTSpBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ={TDATA ={~DATA ={L@DATA ={DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVoc~FWO_GATCFeat//~GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISCP1AMEZP1RLEV'P1WDE_;P2AMEhOP2BAEElP2RLEcPBASEEFDPBASEECPCONEESPCONEEQPDMFTPDMFCPLOCEMPLOCEHPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%CSCAN@SMED SMLtSMPL##TySPACAhSPACCSPACAhSVER4.0SVER pSVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHCphDYbigphQLcphTRphTRSKbS_58LNu~)B*HUrG\W\|JLw?S15Qc4+}9~>26AEMT3pb}045@u:r*nOroz;P]]~eYA}MS8 Xo2h{/S9~i,RU`Zjp2 9c}LVFXj|udrVbH`6@m rQ%`)v42`n4`V4{|L&H:z9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,HKW>G SCe`rlx%b@cTY_eM6^sO \}!&R2{bP#<,\#_B4?_'NBz#hlvX`snWhBHIL+0su,fIs6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(":?S`,j.uFTcM#yKA#2rBA++m}Il4N&u|M`$ iwGU*:87B (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flt78{uU)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#