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!)((000777??>EEELLLRRRYYY__^eeejjjpptvu{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1029bddafd7442088fb4fdae708c13b4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_03-34_07613130Seq16820134913_134913mfagan96-Well##"#"#"#####"#######"#################################################################"###################"#################"##"##################"########""##"################"######################################"##"########################################"#####"###"#########################################"##########"###########################"########"##"#############################################"######"##"################################################################################"##############""####"########"######"############################"##########################################"##"###"#"##############################################"########"#"#########"###############"####################"###########################"##################"###############"########"####################"####"#""###################################################"###########"#############"######"##########"####################################################"#######"####################"####################"##"#######"#######################################"#"################"#"##########################""#######"#"##################"##"##################"#######"#####"####################################""##"############"############### K1322_D7_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z).8Ha&7DLPTZcp~  uYA+lK/ #?_#Bd 1Objh]K5l\PG>2#yY8~unha[TNIGGHIGEA=98789::962-($""#$%&'*-1466679>EMWbpAn7a}bAi8 }xvx}Cl$Nu+B` 6\v{`2L<f9%Er@(gv0'}5jZMA81,++,,+)&$$$$# !&,146788:=EShZ%UqwfBf-rVD;8:>EMXfuw_H4%        $)*($$Av:h"nYu/ T' "$%&&%$#      #'('&%%&'&#      !(.47862/3J~j"2/t{?"#*06<DKT^jyy_G3&  &N5,G7CN("(,//+&  !#&()($  !'-1332112330-/>d $/wD=ZW\! 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T b@VAAM2AKB.bcpKB_3730_POP7_BDTv3.mobtermAGAAGGTATGGATCAAGCAGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACCAGCCTTGATCGTTACAAAGGCCGATGCTATGGACTTGAGCCTGTTCCTGGAGAAGACGATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCGACTGCGTATGTTAAAACTTTCGAGGGTCCGCCTCACGGTATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGATTGGAATTTTACGAAAAAGAAGGTATGGATCAAGCAGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACCAGCCTTGATCGTTACAAAGGCCGATGCTATGGACTTGAGCCTGTTCCTGGAGAAGACGATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCGACTGCGTATGTTAAAACTTTCGAGGGTCCGCCTCACGGTATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGATTGGAATTTTACGAAAA*8BRcr&1AN[k{*8CP^iu (3?KWan{ .<JVanz '3AMXco{,:EQ^iu #.;IUamz '2=GT`m|    & 2 @ L W d r |    ' 3 @ L X c n y  " . ; H R ^ k w   ! , 8 D P [ f q |  # . : F R ] i u '3?KWdoz*5ALXcp| !-:EP\gt %0<HTaly*5ALVbn{+6BLYdpz)5ALXdp{(4?KVamw "/;FR^iu  +7BMZep{'1=JU`lw ",8EPZfp{#/:CNZcpu*8BRcr&1AN[k{*8CP^iu (3?KWan{ .<JVanz '3AMXco{,:EQ^iu #.;IUamz '2=GT`m|    & 2 @ L W d r |    ' 3 @ L X c n y  " . ; H R ^ k w   ! , 8 D P [ f q |  # . : F R ] i u '3?KWdoz*5ALXcp| !-:EP\gt %0<HTaly*5ALVbn{+6BLYdpz)5ALXdp{(4?KVamw "/;FR^iu  +7BMZep{'1=JU`lw ",8EPZfp{#/:CNZcpu   # #)' % %!::1':*0:/16>>>>>6).%>>//>>> '8/81,83>.881%>;>>>>>>;8>;;>>;>>>>>>;>>>>>;;>;;;(8;;>!>;>>;>>>>++>-3-+>;>>>>>>>>>>>>>.>;>>>>>>>>>>;>->>>;;>>>1;;;>>>>>;;>>>>>'"2+>;;>>>>>>>8;>';&>>>3+-&-,+"%&;--&;>>"8. , """>>>>>>>>>>>>>>>>>>>>>>'&*3'>*>>>>>>>>>>>8>>>6**&%&33%& >>>88,883>>63>>>>>>>>8>6>>>>>11>>>>>>>>>>>>>8*!&83>>>>>8>>>>>>>6*28/6/>>>2*! 8# !*>>>>>>6>>>>>>>>42>4+4..44+5')66*64:6:::4%:%'/466::4466!6.*:5.:14:4)//:32::43.#+(2:3%::06:4:::.:,: )2%' /'1):.4441%4#:'((%2#!3!$!5*%   &!     # #)' % %!::1':*0:/16>>>>>6).%>>//>>> '8/81,83>.881%>;>>>>>>;8>;;>>;>>>>>>;>>>>>;;>;;;(8;;>!>;>>;>>>>++>-3-+>;>>>>>>>>>>>>>.>;>>>>>>>>>>;>->>>;;>>>1;;;>>>>>;;>>>>>'"2+>;;>>>>>>>8;>';&>>>3+-&-,+"%&;--&;>>"8. , """>>>>>>>>>>>>>>>>>>>>>>'&*3'>*>>>>>>>>>>>8>>>6**&%&33%& >>>88,883>>63>>>>>>>>8>6>>>>>11>>>>>>>>>>>>>8*!&83>>>>>8>>>>>>>6*28/6/>>>2*! 8# !*>>>>>>6>>>>>>>>42>4+4..44+5')66*64:6:::4%:%'/466::4466!6.*:5.:14:4)//:32::43.#+(2:3%::06:4:::.:,: )2%' /'1):.4441%4#:'((%2#!3!$!5*%   &!  Z@N #"*!a9:U^=>H@: @EY%:( 0 G%CSOR,:?_ [9G*/`BFGLF7$ SZ:)PT4*KG :/BJBD.Q4Q J*>)E9XEY 316.JDK0X@.ECG4CGdH U :-$Q0\5#U`;)4:' 0 \,"`NJ`d?*C!W K# NV2PA 6Q@)EBG9$PU8$EOI_H0@266!##&D`<*=P&KB , 3@_#P/W/$GGQQ<3@)+\0"(6F;Z]0PXF>@O ;b/Q:'[0T1I.; 5< ?A@OG)&=V=$304NQB 9])=MIc_:6[U\UV-S cSW2B?' _&U.Z9[*^5G:*_ (&2K8Y)O%;F2=IAW<7Q"Y_U+,RA$< LP?]50K B'!. Y\F7+(D.d /b(,;#,(I/RW'MJ6H8B(3Q)<!'b :@SJQH7*a3+ U YVY-1IU=I<+P `) G?5WK 0+&"k ^p@+1k65cL H1g--< }=E0n4s` q]VsG= 7XT  gFriNKwUZf` oX< Qy!qafSh7mui _}"_OkC%"bdeLSY haD:Y)]cG `ij K\&|C (wr=9xBr3>\O qP+f+XMH&Er5g6;fd-G9s}DGi*k1?vtA'HaE @{DF=kF i.U|4nCCQqKCk F0Ie 4Wc JA`Ll7<SF/=0J], cbjPdiBh Ngzv:e]l' c\dE %b70 {aVwWLZIIs]! yu~|eajp]Wm_^SPKI<Sekw1;O=C-+#..85'8.' '3"!         5'?, $                      !          ++ ,')&"+!$&)9++,1!%(BB(/%%3H*6.50#..@:-+B7&Gk{HjOSeK9P S@@".E]Q4%MSGRaxeSXmaC\dVKPgoOargPFWwQ`Xbd|Omzf{f[/R:Xd2kR4} }8zUUqyUU[?kOha@rgdhi\r\CUUnUS\^dmovFB,UU]]ssUUUUs\&sssspdp=y_5$#jCp18w<G;kp p4j./LwMgV.Ht/2Kxd|!dEP$=:d }rVj 6Gd8UddZ/ d{1edK}':Gh-hndF M,=WqmdW _lu6-UJ9K) &E8p,m IWE: j6ppg*pp6NpHp,Qd ! ixOS+uCz]08g@ 4A#m$[!mm^S!;5!E7i)5g&(+J&($v^0D*.))3z"2"$6 "I;/%I*#[4-4*&I7%*E)*1/2 ":. 9+%)-61KB4L/='G.6 [#-$05 /#y&1 3),'M%/*2+ 3'"#2/ !c.]L=1+21$-F'A,L.q\/AFj>M(EK3${=1K.-,&2 !**)+!#,##)"&T/7$,-*3/:0"@>%&$)83(+3!"5=,LB?1A&7 $ .1O(A?#%-#(!*/AD-/?)&3+9./1).-;o((-M4=F.',=bU::l^`I=0+">6S+'=A!)pW^@aD0PPXJBA.UUT&D/p&ePR_\zZ"]YH}YM_rL6*$cK{yQ6p0@^6ryYt+Qov"/[y{-14o6,My $1EmGAGGATGTGTATCTCGAACTTGTGATGATAATACGTAGCCACATAATATACTTCAGGATACCGCAACGAATACAGAGGTAACGTCGGCGTACTGCGAGGGTCTTCAGCATGCAGAGCTAAGTAAGGAGGAAGAATCTGCGGAAGCTCCTGAGTATCCTCCTGTTATACGAAGACCTGTGGGTAGGGAGATAGAAACTACCTTCGTGGGCAACACGGAAGGAGAATCGCCACACCAGCGGGCGGGTGCAGAGGAGGAGAAGGAGATAGCGAAGGAAGCGCCCTTTTGAGAGTTCCCCCAGGAGGAAGGTCCAATTCCCAGGTAGGTTCCTTCGGAGGAAAGGGTGGAGGTTAGATCGGCGGTCCGCCCTGTACTCCGGAAGGATACACGAGTATTTTCAGCTTATGTGATCCCTGACTCGACTCGTCTTCTTGCAGGCTACGTAGAGCGAGATATGTAGAGCACGTATGCTCTTCTTTTGTGGCCATATGCAATAATTCATAGGTAGATTTGATATTCGATGCCCTACGCTGTAGCTGGTGATAAGTATGTCCTCCCTGAACCGTAGTAAAACAATCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 05:22:03 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTS2dBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 6 l@TDATA 6 l@DATA 6 l@-:DATA 6 l@zDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~0LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMBP1RLBP1WDB! P2AMB*P2BABB.P2RLB%PBASBB7PBASBBPCONBBPCONBBPDMFTPDMFPLOCBPLOCB yPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPL!!TySPACAgJSPACSPACAgJSVER4.0SVER 2YSVER,,/Scal@Scan@TUBED7Tmpr<UserphARphCHphDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTS2dBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 6 l@TDATA 6 l@DATA 6 l@-:DATA 6 l@zDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab@FVoc@FWO_GATCFeat//@GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~0LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AMBP1RLBP1WDB! P2AMB*P2BABB.P2RLB%PBASBB7PBASBBPCONBBPCONBBPDMFTPDMFPLOCBPLOCB yPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND  RUND  RUND  RUND  RUNT "RUNT RUNT 9RUNT 0Rate 0?RunN""JZS/N%SCAN@SMED SMLtSMPL!!TySPACAgJSPACSPACAgJSVER4.0SVER 2YSVER,,/Scal@Scan@TUBED7Tmpr<UserphARphCHphDYbigphQLcphTRphTR,*^(`P. 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