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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 032d854a851a44bbbacf023674c99396Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################ K1321_G2_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zwsruy|{wpg^UOKJJJJIFC>:64211345420.,,-..-+*)*+-..,)''),021-'!"'+,+($""$',06;CMXes !6J^soS5o]NC<862.(!#*4@Pc{(hIg{x_: i3aJ;2-+**+.148=CLZl  tWA2+'%"   ,Ed 7EJF:& ~]A+#:_,hUj^4f(<gn4=xR7%          -O|)Z{xV)7u]XDqA8H   .W; e{J(         ,b*/| 71CWkL|8HLM'>xn!$*0464,C=}Wf#  )05652-'"       ;:+'!#AK             &p c?x(~,     %*/3675/&y=M&:X7  `oy=Z  M@5|c&I'H;U9&_+\  Zm CFKEs}v["i   (-000022/%  E-i&wF     5x,ynk, #'" 3dK35j        +~[p>J\  NpE> ',,(#  #NYyrA& n1 " $$    Gi*i* &0771'!'*("   3w;\G  #(-10*  O:%N0Q`  <s-g2 `y >? 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B{ )o<" 1vXK}#-k9!        .FfDQ kI0  &1<FMPPMG?6+" #$#     &.7=AA>8/&  @ AADW?A4KB.bcpKB_3730_POP7_BDTv3.mobtermGCCCCCACCAGCTCTACCGTAGTTTTTAGCGGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATACCGTGAGGCGGACCGTCGAAAGTTTTAACATACGCAGTAGGAATTCGCAAATCTTCCAGACGTAGAGCACGCAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAATGGGTAAGCTACATAAGCAATAAATTGACTTTCTTCTCCAGGAACAGGCTCGATGCCATAGCATCGGCCTTTGTAACGATCAAGGCTCGTAAGTCCATCGGTCCATACAGTTGTCCATGTACCAGTAGAAGATTCGGCGGCTACTGCGGCCCCTGCTTCTTCAGGCGGAACTCCAGGTTGAGGAGTTACTCGAAATGCTGCCAAGATATCAGTATCCTTGGTTTCATATTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCGGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTTGGGGTGGACATCATAACGCCCCCACCAGCTCTACCGTAGTTTTTAGCGGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATACCGTGAGGCGGACCGTCGAAAGTTTTAACATACGCAGTAGGAATTCGCAAATCTTCCAGACGTAGAGCACGCAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAATGGGTAAGCTACATAAGCAATAAATTGACTTTCTTCTCCAGGAACAGGCTCGATGCCATAGCATCGGCCTTTGTAACGATCAAGGCTCGTAAGTCCATCGGTCCATACAGTTGTCCATGTACCAGTAGAAGATTCGGCGGCTACTGCGGCCCCTGCTTCTTCAGGCGGAACTCCAGGTTGAGGAGTTACTCGAAATGCTGCCAAGATATCAGTATCCTTGGTTTCATATTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCGGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTTGGGGTGGACATCATAAC'-8?U[cr *5BOXht )3BP\hu%2<IWds+7@KWdp{ #1?IT^jw %1?JVamy +7DR]ht(4@MXdq}    ' 5 @ K W c m y    * 7 C P ] i t    % 1 < I V a l x    ) 5 A L X e q ~  # / ; F R ^ j u )5ANZdo| $1<HTanz !,8EQ^iv*5AMXdp} #0<HU`lx -9FR^ju*6BMYeq|$1=IVbny+7DP[ht'3@MXdp{ ".9EQ^jw -9DP\gr~'3>JU_iv '7'-8?U[cr *5BOXht )3BP\hu%2<IWds+7@KWdp{ #1?IT^jw %1?JVamy +7DR]ht(4@MXdq}    ' 5 @ K W c m y    * 7 C P ] i t    % 1 < I V a l x    ) 5 A L X e q ~  # / ; F R ^ j u )5ANZdo| $1<HTanz !,8EQ^iv*5AMXdp} #0<HU`lx -9FR^ju*6BMYeq|$1=IVbny+7DP[ht'3@MXdp{ ".9EQ^jw -9DP\gr~'3>JU_iv '7   !1!$'': **%::&$::-":1&>$+>>/6>>886>>//>//>>11;'>;;;;>>>>>>>>;'>>>>>>>>>>>>;>>>>>>>;>>>>>>;;;6>>>;;>>>>;;>>>>>>>>>>>>>>>>>>6>>>6>>>>;>>>>>>>>>>;;>>>>>;>>>>;>>>;>>>>>>;;>>>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>6>>;>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>;>>6>>>>>;>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>;>>;;>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>;;>>>3>>>>>>>1>>>>>>>>8>>>>>>>>>>8>>6>>>>>8>>>>>>>>>>>>>>>>>>>68>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>*>>>>>>>>>>::6::::::  *    !1!$'': **%::&$::-":1&>$+>>/6>>886>>//>//>>11;'>;;;;>>>>>>>>;'>>>>>>>>>>>>;>>>>>>>;>>>>>>;;;6>>>;;>>>>;;>>>>>>>>>>>>>>>>>>6>>>6>>>>;>>>>>>>>>>;;>>>>>;>>>>;>>>;>>>>>>;;>>>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>6>>;>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>;>>6>>>>>;>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>;>>;;>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>;;>>>3>>>>>>>1>>>>>>>>8>>>>>>>>>>8>>6>>>>>8>>>>>>>>>>>>>>>>>>>68>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>*>>>>>>>>>>::6::::::  * $;%Q<aO/cT V>\*U=O/:>Zc)!;[NV*&BNF [:W![,XMa#_HBN])+4PY' [D9-d%7QKE- #^AH/RIC. T+FXV !DFZ.T8D8%^P]D=I ,/)X >B]5%JFS59 (\NSCM(-%^Z 4_B= J6QQ,' JO(B`76HU ""L<# 6 CPO]1$K3!F)["J]_F)0 J`c8>KXA[ D0aG6b0' ^!<3a1d6G# 6U:;Z"1;15 F =H _`SACOLPNGZTR" _6F,%B!;2N[O`(>,Q3b#^$bJ% MP[Y?> 25.G@:*&6YcF;*A/_) IYR9FO4$\?[L"%^ C"S>4L&\+098DK?"bC: F-5'"V. &>S) +ZK\V,8c5_5#Q!E- K5/Z) 3=:R) >9ID"5\-XYSFR% I2P( A )[N4 .YW'\7: 5QFdS:=<,cM<SHR)'NT%T_{ "':s8TpLe]sa d]_D } fD+T&)?D`F0Rr8N$={ - ShY.F  ak!4ecv p> 2+ e"A= OG}h&]cQ]{qkpq(F EH h4e` yV;s7g0]kER73G-M!HO#OL)c7_3pnG$ R|W*8rM|L@ mC0`+g:dwE J>IH,1p N )PcUmN; .-qjTvD 9dP^\.]&GuSj3f Dg*kl$_cEKR{~]-,QDljn[YZBpY<.0(4Z 7  ) xl:2+;gAntuUMRkZN[c~K8&76:)7)$2 3  '(                   -    ) !+   " ,1, #+*,-*)47,.!&'%    " p,pNpRq:v4rQXj#p8IDEph(b<g8zL$4N,O88'$pE3GYFMfu%Pt#?lp=L:'^*%;%+z6 dT?R7LpkP~+byye*sPsn^4#6up|3!pdQu,F8Cp8w_8qp!Tdai(p~xps1~}l*pW/$9BppLB t F4TCqb M5#6N/);N, |*3~7+1;Rn3 f;7V95>-)M% ,{ 45&!<!3$ $$ *63%"Z.% )16#+.!19&3*B&';"##+$$",32# 1>I-; ) 6%#3!,%0-6( -$9D6l!*3%-+(!#$ ,D%( ((/!%68M32!+(',!B&*-%-#$"/$72&3hB'#9"$I(,1+14&/1;&$<14('19%%)$G($%J#68%!&24>$"E %+& &/Ap.@A)%&5%!!/-,4 &)" $/"*31KT0$*#'B5%'E'?&;(, 9+%304'-+#"#*&8o-* ,+!,4'!$(3L35[V `NM5)} 71cWAAAAAATAAGATCTATAGCATAGAAAATCGCATATCATTACCAAATTTGAATTATAACACCGAACCACAAAACACCGACCTTCACGGACCAAATCTCGCACACCACACATCCGGCGAGCCGCCCAGCAAAGGTTGGACCTGCAGACGCCGCCAGGCCTCTTCTGGCCTACAGAAAGAGTGCACGGACCTCGGACTTTTCCGACTTAATTTCTTCCAAAGGAGGGTGCGAGGGAGTCGCACCTGCCTCCTCGGGGAAATGGGGAATGGGAGCTTCAGGAGCTAGGTAATTTCACTCCTCTTCAAGGGGAAGCGCGACTTCAGAGCATCCTTCCATGGTAAGAACGAAGCTTGGGTGTTCATCCCTTCATTGTGAAGTTTATGTAACAATAGGAGAATCTTAAGGGTGAGCTTTTTCTTCATAATGACTCTTTTCTTGAAGGCGAAGAGATTATAGGTAATCCGTCCAGGTATGAGCATTCCCTGCCTGGGAGTGCAGAATAGCCTGAATTGAGGTATTATCCACCACATCATCAGAGTATTCGACTCCAAAAAGTGTGTGAGGAGTAATGTACATAAGATCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:33:41 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSe]BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA <}xTDATA <}x͌DATA <}xFDATA <}xDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~cLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS8P1AMEOgP1RLEJP1WDESP2AME]P2BAEEaP2RLEX{PBASEE:PBASEE8PCONEEHPCONEEFSPDMFTrPDMF8PLOCEAPLOCE=?PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%8zSCAN@SMED SMLtSMPL!!TQSPACAh3 SPAC8SPACAh3 SVER4.0SVER eRSVER,,/Scal@Scan@TUBEG2Tmpr<UserphARphCH8phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSe]BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA <}xTDATA <}x͌DATA <}xFDATA <}xDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabsFVocsFWO_GATCFeat//sxGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~cLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS8P1AMEOgP1RLEJP1WDESP2AME]P2BAEEaP2RLEX{PBASEE:PBASEE8PCONEEHPCONEEFSPDMFTrPDMF8PLOCEAPLOCE=?PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%8zSCAN@SMED SMLtSMPL!!TQSPACAh3 SPAC8SPACAh3 SVER4.0SVER eRSVER,,/Scal@Scan@TUBEG2Tmpr<UserphARphCH8phDYbigphQLcphTRphTR'%N`!2FPu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ou{,U#LUq.}tmBE++r-]3:!h]R_s_beT 1"/"%)O~exg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*IMB^4}rJl6d+lbf*yA=$I;i/cE`\P dhIW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^i1>QD/Nx6;\JBQL;gPb[<)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#