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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: edb0bbc2bf8b44e8923073ec0242eb7cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################ K1321_F2_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z~xsomkgc_\[ZZYWUTTSROLIHIKOQRQMIDA>==?@AB@=:779<@A@<60,++-/247:>BDEDB?>?AEJNQTWY\_adfhjkmpv&Ny<c:R]ZM8i2T.{wy}yvvxz|}}}~~}}ztlc\XX[`hq}3['Sr}p^I-b&{iWE5+&(-5<CIP\m%%~iTB1$       1O} bffGzT:+(2KqY 8T[L,TKddA-%%)++($!##!      &2D`0f[5!-ech{F&\B  4Z. $3F     !" !$&'%#"!"$'('%" !$(,/1111236=KexGN-E /[7GT@Vg/j'.l#:X>Y Vj;-3@KQQMF?8300149AOjB+VPo,  &05642.+(%!#$"   !"##! $Q\Q~#(m(   )2650'            RrOH#Mnrw+ .=FF=.   5H 81  &'%'? ~D !/42.+.>e0- "c "fW"!2Y7\!x'b+%HU8VyO    %((&"   WXo`c.  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UA1-/IC),DGAB63 NX: YP_".\M?:7."7SOC&T@"%CcCc P75G7A@`D@<J(6@A` %1/O%->SNO[9a.<a(XVFISO<(X0F: K$30 ,0 Q#2WJ9KdD_(UD($@#VZ<d^FXCO.TG ,B-`JADJAZC0\:'YW6+M.,! ,86J7I;B65'T0\?YaM$V5!T 1(:3/!`)IU`:.O5d"%; a0 \B61)C?V"JY' _ V%_Ba CL.>J0[*A2!ZcT4,V+5R'4@T;ZZ,R6%)L_ &DC$48MOR)PJ#/'*AG\:\2L`:V//OX]-$A9CBdC7d.Q5S )O?,*V;a2Jc_b (*J0$A%R6- Uz+nQzF+NU{:Xc>f'Rs 8 I^i?r~5Y.]'N_}"[EecAhguAU yZQx=f"oVMd~v+C nZope  2| yAk~bc:${XGc75r],t #(+{FYCBk(9*-1=W8_GHN[IXy&S#L*0qPdIF;Zb*F@$ BgyImj6A %&5<Qzx&)=Na <;xI=_O-,B='M` jc69pV6y 0Um ~j? ^,`kQMP n?DM L}!UUpX`*s9M(cji\hs^YaVOI:TY>:62=>@&>;/&#0170+             "           # #$!1/ %"#/#! #,/ %"! "&+%  "%$/4119,+*??)!,C6/<0>8?+:>%*-2V",+).'''''$.$$$$$$, ,`zppE +pZ,Zp|drZ"5,?>+XHq/)VB d,y%[ ,6`Q,I@q H~B,8pBd2&owR7Ad%/ k1bvGSKy]K%}29d5I{Z.vG-Q" dfLSdrvn5~/I8Ip|/H6Bd,WpyhdrpQ{1N8{p' 8?8%d!\UVLp,83p9,dppm] U<})3RDSasP&k+s/!B%4>#&JHUDF?6!EPK-"l28  !K; =%7(<**)I%/&)"# "++ %"3#@(K; $'#'( ?%+, 1A'!; & &.3%;,0-';0-6!, ,%!$,0#F!"$-, `GWj(D</%1.-3.#&"-'@54<#D%1%P*1 '* &!9$#%G&59"*+#/,.:H7%!**# -$%%*=1R#@ 4;^8; 4)"!&/!2!" ,)=#D!'5#"!$5E8 2)(&.# / $H(4:#$#F,-<1/:##+@(9)JE+fC?D@8XD//94AAAATTATCAGTGCTATAAAATAACTCCGCGCATACTATTACCAAATTTGGATTATTACACCAGACCCGAAAACACCAACCCTCGTGGACTTAATCTCGCCCACCAAAAATGCAGAGCGCCGCAAGTTCCGTGGAAAAAACATACGCACCAAAAGTACTCTCCTATTCTTCGCAAAGAGAGGGCGGACATGGGTTCCAACCCATTTCCTTTCCACGGAAGGAGGTTTTGAGGGAACGGGACCTTCCTCCTCGGGGAAGGGGGGAAGGGGATCCTCACCATCTACATAGTGTCCCTTCTCTTCACGGGAAAGGGAGACCAAACCGCATCCTTCCCCGTATAGAGGGAAGGATGGGTGTTGATCCCTGCTGTCAGAAGTTTAAATAGGAAGAGGAGAATAATCAGATTTCTGCGTTTGCTCCTTCTGAATATTTTATTGCAGCAGAAGAAATTATCAGCACGCATACCACAGAGGAGTATCCAGAACCCTGTTGTGAAGAATAGGATGAGTCGGAAGATTATGCATCACTTTCGCCAGGTATTCTTCACAAGGAAGAGTCTGTGGATTTATGACATAAGCATCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:33:43 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTSmBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={TDATA ={ϨDATA ={JDATA ={DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~lWLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AMFWP1RLFSUP1WDF\mP2AMFeP2BAFFjP2RLF`PBASFFCkPBASFFA%PCONFFQPCONFFNPDMFTrPDMFA PLOCFJ=PLOCFEPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%@SCAN@SMED SMLtSMPL!!TQSPACAg8SPACASPACAg8SVER4.0SVER mSVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCHA phDYbigphQLcphTRphTR7This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTSmBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={TDATA ={ϨDATA ={JDATA ={DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab|;FVoc|*FWO_GATCFeat//{GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~lWLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AMFWP1RLFSUP1WDF\mP2AMFeP2BAFFjP2RLF`PBASFFCkPBASFFA%PCONFFQPCONFFNPDMFTrPDMFA PLOCFJ=PLOCFEPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%@SCAN@SMED SMLtSMPL!!TQSPACAg8SPACASPACAg8SVER4.0SVER mSVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCHA phDYbigphQLcphTRphTRc( !j rh[Z66YM'KvT+F4:H[);@#PA+eg[gUGGN*+j:xUIc.jCxO{VZHngM0xW#nZE35ux2]DM6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(Y`N!:X>zt %v1${NW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`ht1A'obU2kR@ef&e}hw@q}%Q`ALIghJ*(k;:|e}OM *R@_>j23T6Y/Xh]'p8Npdo(ktnP_I[Dd@2g|5J_@A[]"p64y^M_9ni|\Z&,NlI]<[S\nX\i?g?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!39G,RS_%ssaP8/GC.JuCglxsQlnWH%`WZ\9~#M.~CZ Gu%o;MmF*?EgED0V31K}i>ZIBNT,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS`V2]<|':eqB?k3Pmt(7U+4]B*Zvh<=mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6L`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt)ASPt)AUDTNB1Pt(B1Pt(BCTSmBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={TDATA ={ϨDATA ={JDATA ={DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab|;FVoc|*FWO_GATCFeat//{GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~lWLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS@P1AMFWP1RLFSUP1WDF\mP2AMFeP2BAFFjP2RLF`PBASFFCkPBASFFA%PCONFFQPCONFFNPDMFTrPDMFA PLOCFJ=PLOCFEPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%@SCAN@SMED SMLtSMPL!!TQSPACAg8SPACASPACAg8SVER4.0SVER mSVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCHA phDYbigphQLcphTRphTR