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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 21191456e1ae470c84afac9976870a58Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################!K1321_F12_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zrssqmha[XWWWVRKC<74468::::;<=<:61-++,.//.,*)*+-0110.,+++-..-+)((+06;=<94/,++,./258<?A@><;<AGOW_fmu$+2<GS]b_UD/udVJ@7/'! 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W &TNE\N^$(*O ?CC<J7TBB_- ?/V;! N=AE[4XN^'SI@541(b>bVE\ =]4)MY ?bNT ;!>0TE"*I%0)EUTTQ9OT(CJ0SI^P\A'(TA/NTWV)$L^ G@35FP>%b-'  5 '(< WQVI`F44V=Y7 /= Jc1&#.(/7.\>ES^^">[$TP40+ 5b'!@EG^;^8X0DWDEL_#[W= VV8( RWB7X(N%I&7AE(9aQS K`&1JcRRRAO3XM7R 21#PR2T*O<.<&T"/=c+ d+"E 'PN7L\Z]/. ]5@bW_D IT%I%!D`%HP [N.6F<]b^9b)UHB@N3Bh aO&b9YhYCMhB|PFGm 6u+c?L .krF{;o =G2w,jH69EYBjzk8;1 u"ZnL jv*%U*sf}+, _u52m!F ,:/Q*Z<h72%o._[\Oo7 kKQ1`N>?w{3$?5P[ 3nZo6) _ Q ,F^/8RgoTv.Kbi5VS  j|w ( _2+wgX1^<~"~Di=IFT8tUWRvwx!*:YPg 9/!T4HX6h\3`L[(cY NW$vk&r\`;Kw j Z #~ s~34pw& A^)th 5yL*Qcjfi_q\vPEA:MF=F>7,-*3?@45!53*( !!                            & "$5# $>$!" !!#)"%-" ('&+:(2$ 4.1/*'?<@(/51 (!"<$*&)*K$$$pp2JpJk|Z#+mu=2/ VH8rd$+SpyJFo=r|Wu@p)o oD#qNQdpQ!E<}Z .~q)d OX>pH*1 GB,PZ%VawPcxI)P;{WH[dfh |XM gq"z 8RrpXMU8dH@8'EdGMS8q`Hp%pp)6O/dI+QGp=.p4B  NUC]z Ec JS^;T$>|3{k0)e%#8%9!<(Gi>Xp'?9#03H0&  Yg!3 / '"!(+&'7"6 '($#2**"( *(X  /5H%$4"("K00 3:"4*;, 8 !%% A 2*# B#%*"#3$7%" $5$")-  "E%)% $#&=-- )%)1=#!"'*,+)4.00#!"".;+)*&% & !/1. $*41!1#%$*4/ 8/($!5T"(2!!#&(/  &969-#:$:&"2HKc+GC/(=',/#& :"(,!<1*'-,j($$0&8)1(8%/'%! 0>P65%,+%9&/S*$B00,*55''A(cF">H( ,6#i/<,Q+ ()'.<G*./QQ=C>?VI[.v0 IAAAAATACAAATCTATAACATAGGCAATAACATATCCATATTAGATTAGGATTATAACACCTGACGACAAAACACGAACATTCCTGAACCAAATCTCTCTAACCGCAAAGGAGGGGAGCCGCCGGGCGAAGGAAGGAACGGGGGGAGCAGCCAAGACTCTCCTATCATACAGACAGAGAGCGCGGAATGCAGAGTTTTCCGACTCCCTTTCCACGGAAGGAGGGGGCGTGAGAACCGCCGCTTCCTCCGGGGGGAAGTCCGAAAAATGAGCCTCAGGAGCTAGTTCCTGTGACTCCTCTGCAAGGGGAAGCTAGAAAAGCCAGCATCTTTCCAAGGGAAGGTCGAAGGTAGGGATTTCATCCCTTCTCTCAAAAATACAAGGAACAAGAGGAGAATCCGGAGCTTTCGCCTTTTTCTAATAAGGAAGTTTTTATTGCAACCGAAGAAACAATAAGTAATCCGTCGAGATATGAGTATCCCCTGCCTCGTAATCAAGAATATTATGAAACGAAGTGCTATCAATCACAACATCAGAGTATTCCACACAATGGATTGTCTCTGGATTAATGACATAATTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:34:58 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtJASPtJB1PtIB1PtIBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1cTDATA 1cDATA 1csDATA 1ccDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~tLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AMB"P1RLBP1WDBP2AMBP2BABB 2P2RLB*PBASBBPBASBBPCONBB\PCONBBPDMFTsPDMFrPLOCBPLOCBPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAmWSPACkSPACAmWSVER4.0SVER SVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR5This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtJASPtJB1PtIB1PtIBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1cTDATA 1cDATA 1csDATA 1ccDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabXFVocGFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~tLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AMB"P1RLBP1WDBP2AMBP2BABB 2P2RLB*PBASBBPBASBBPCONBB\PCONBBPDMFTsPDMFrPLOCBPLOCBPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAmWSPACkSPACAmWSVER4.0SVER SVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTROp]C$xXO+lkfc40-fKbVUg|=a1tirD:Z5A>9^kZdg~WI|Mb;eX}"if32#/P[~W!Fy^q3Th$Y i ^UO ]ddc}?8d)sGi=&MQJ1r]ZvZ~h6dYHa?yh;}S$PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AV^V4?HV;mUCBM[?|$}d& 'iJ4~CubW_]?ZC'T3T&#ONx8LkV}d7?#)io5`N0W`,#Xr_<.Fi1A+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\W\lWQ7M3gptQ>?";!8L[2Rr`qHT^1R=goQS-CDiyX^UM]N=?W.ss$T!D@4ir$Gj~`? MlYXjlnK[Yl`A #pJE,#VuU4zrfv4AAnE/zK{=+nV:W\Yh>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#j1DjU5]DtmXY#agsC!.:QI5&.XRz+*}Lq&Wi1W#]Z@4}rJl6d+lbf*yA=$I;i/cE`\P ~kIW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^i1>QD/Nx6;\JB]LvQP':O)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtJASPtJAUDT-kB1PtIB1PtIBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1cTDATA 1cDATA 1csDATA 1ccDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabXFVocGFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEVLAST~~tLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AMB"P1RLBP1WDBP2AMBP2BABB 2P2RLB*PBASBBPBASBBPCONBB\PCONBBPDMFTsPDMFrPLOCBPLOCBPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAmWSPACkSPACAmWSVER4.0SVER SVER,,/Scal@Scan@TUBEF12Tmpr<UserphARphCHphDYbigphQLcphTRphTR