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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 64013ea1d50a411694e311402c59c0bbSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################ K1321_E5_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z}yvsokfa[WTQOMJGDA?=<;:7520.,+)(''()**)&" "%''&&'),/0/+'$#'-4:?@?=;;=?BEGJLPUY]aeipz2UwvaE#l^SJA92-)'&%$"  $(**($ #)/331.*)*-14542/.-.023431/,+*+-/234555542/+('(-5@N^rF{$V|j<NlUD80+((+/2579;>BFHHE@93.*))))(%! 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AG$XMT)@Ia>BQ+d)]'5HO:'*WS79CVFH> I6H!OR(9>Q7`_2%(?@? 6@(:5))QD%C22\BE9-!&/4:'+1 @GGc8##0O/DXXVHb7D4?37?7N#3 ]&$ 6!30& G#6<K7/),=FGI<XDRR$BRF:U@$'=CG#9O<TTJ%^35;U/&+QB* TJBM=V*<"cR&X0X3<"9@0Y,5'L 9O9N"55B-G F4LJ2SI*>N&DFa G3 ,X8D9 I\"(1NN0**b6P8`)UF;4-F#4:2?.*;BaI^A#65 1A+/'8^VX:C+>2(TG6`3LX'sPPI]f}POX)1Zg2_{  _u)j"J|7a2ub9%'9 nfAeBRhl5wBe;JC=:4 Kh -[s| 0<6z-A8/"RE75f ]-ZQV-+vu*2$$x\:VHGC{5%b=&N9ZJf|d(U B! ~Jny_!b] )~& ;1X )xEoT83&n$)F D/vfnsrt\!qj$-.E8Y( K5oEEK,5/GTd+M kH0[.dz p",vvq@" a]50B'}#OC2EW)Eo>:%*OJ.)'vC0 51. )% !            #  3              "% '+! ! ! &$(" )G")  % 9)&.6#)/035(0-+,;%,(69411/6/&<@>23M>6B"%(0HQ/BL*1@DN-*8:2*-/[*:+;%3))))&&&&)&242) 22}2226pv.p0,X4 8.d,d{s8 8= +\ ]X?At]tdM8O8I 4$j(R8Kyz _/ 2q"M#Ddd  x^"CI&CyM}ur7dd{88fpLF\Nf cK8U 0gk 898h<$ R39xdxNd`z+8GJ,s Spr {#,_,QNW7dt9/ <Gppr][X p,h-pp5R*@p&wZ "# -?@5B 2:!#yL\#C<lJu1+1U6Y'38 +O/ )''`/'!$ 2-5'#7=&'!$!#()&.I"613W*1:( 0 $$'1'6+;/>!)$"%%%H(!)*--"+PN)N"*+*(S"3(',-!!)*( %$, "(1"8G8$1,-+%)1,>%)& $!&/&*)B.@C5/*@(4>.3*.&-,O(1/&% !( &-!;&*"P#!4!/97+6%*#0-!BYV02/ & I'%(+J0"4%+9%B*I &8MS_7$D29-#@;5A<'9$4"/"@N$>#,115!7.9.(K"3)251.6G.=-+G/@?7#$708=#>(3/,(OC(.-=;1<9'##'"'-0GM%-9&4,.($C5:"hf O91*|l96|)&CAAAATATCAACCTCTACACATACCCAGCGAATATTATGACCACAGTTGGATTAGTACACCAAACCCGGGAACACGGAAACTCATGCACCTAACCTTTCCAACCACCAATACGGAGAGCCGCCGATCGAATGGTGACCATACATGCGGCCAAAGTACTCCCAGGTTCTTCGGACAGAGAGCGCGGGAAGGGGAGTCAACCGACTCGATTTTCTCGGAAGGAGTTGTCGACAGAGCGGGCCCGTTCTCCTCGGACCAGAAGGAAAGGCGCTCCACACCACATATACAGTTATCCCTCTTTTCACGGGCACGCGCTACAAGATATTCTCCTTCCATGGGGAGAACGAGGGATGGGAGTACATCCGTACCCACTCAACTTTGAGTAGCAATACGATAATTCGGAGGTTGAGGAGCTTGTTCCTCATGAAGCTTTTTTGAGATCCGAAGAGACACTCAGTAATCCAACGAGATAAGAAGATCCGTTACGTCATTGTGCAAAAAAGAAGGAATCGAAATATTATTCATCACATCCGGAATGTATTCGACACAAGGGCGAGTCGGCGGAGTTATGACATAACTACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:34:14 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XSBDATA ?XҚDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}LLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMEhP1RLEdUP1WDEmiP2AMEv}P2BAEE{P2RLEqPBASEETrPBASEER-PCONEEbPCONEE_PDMFTrPDMFRPLOCE[APLOCEVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL!!TQSPACAcûSPACR SPACAcûSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR(phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XSBDATA ?XҚDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab0FVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}LLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMEhP1RLEdUP1WDEmiP2AMEv}P2BAEE{P2RLEqPBASEETrPBASEER-PCONEEbPCONEE_PDMFTrPDMFRPLOCE[APLOCEVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL!!TQSPACAcûSPACR SPACAcûSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR(phDYbigphQLcphTRphTRGP58Jr7y!{_a1z{,c]n{uH<6#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`U*{Zq9FyhCebT8Poo=[/19~{2A~&!sR(&^iCq=dGE^E:DH6[~=7?xh?'kT'_C:q|4G,fX-N&Wv'i2{M~V\J%b?Af:36nMekWt+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4/ VpP`w1*{d:7/Q_nj3{:F^LBE3Sk-[qr[&X1EDE?]mnjH[$.}RZ#3|5O-U[d1DFW%j'kT'_C:Or4^7~s?K90lVSFsA bXX<,x'EEwiOfFWcDArh]|Ko5qS+SP7T]Tcr[&$&?_1OPh~jTn/])RpikE:b&b)>`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFUTT3T&^%FyPC,_,qz7A*k|_LhvHZ&qIllp&[S{;$|LD][8LY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d GoVS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTCB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XSBDATA ?XҚDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab0FVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}LLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMEhP1RLEdUP1WDEmiP2AMEv}P2BAEE{P2RLEqPBASEETrPBASEER-PCONEEbPCONEE_PDMFTrPDMFRPLOCE[APLOCEVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL!!TQSPACAcûSPACR SPACAcûSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR(phDYbigphQLcphTRphTR