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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bb90a27a6647496bbac8ba1dd2b6de5fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################K1321_A8_Solanum_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z|xurpmkie`[VROMKIEA=:99:<<=<:8643346787520/0258:;974111367875311358;<;851..17=BEECA@BFLSZbjs~(;Oaoz~yrh\K7{j[ND<72.)%     "&*.00.+'%&*2=L^tI3[wmM!r4eQA71....,)'&)0:GTbp~  z`L=3+%   0Fb%36.dL9+"-@Xs +)dL7'#&())(&#          )<\-Yw|eAq $+@.TZ       1]6^m8        !!  !#$#"&4TD+x  3^xv~x5:of;euD /AhR $+037:<=;72,%7vbVu4  %,3:?BA=7.&      #'*+' >B FU !$$               "%rV&LEovUS  (--)! 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L/@ha8# Lo )R'_a-cfD4,&    :u_O}N2#    !Z+!qb &fR|0S<3/+& !!          ?a=.6K& N }4S@8Q3$      B?] {N0    =I ,Y7$&gIQi|jD0'      5pogW7% :u!|2f 41YrK4&  $SDKpG."     ,a3VJe H"6+aD3)!  1h[eZ8# *X?n%_;&   OGCi@'     &\Qxl0dd?.'%!,ORT\2ymA)    &:Ro/Tt]0}bM=/$  !(.49=@BDDB@<83-'!  #)+*'" ]@oA?}AJl"A =KB.bcpKB_3730_POP7_BDTv3.mobtermGCCCCTACGCTCTACCGTAGTTTTTAGCAGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTTTCAACTTGGATCCCATGAGGCGGACCTTGGAAAGTTTTAACATAAGCAGGAGGGATTCGCAGATCTTCCAGACGTAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATATTGGTAACGGAACCTTCTTCAAAAAGGTCTAAAGGGTAAGCTACATAAGCAATATATTGATCTTTTTCTCCAACAACACGCTCGATGCGGTAGCATCGCCCTTTGTAACGATCAAGACTGGTAAGTCCATCGGTCCATACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACCGCGGCCCCTGCTTCTTCAGGTGGAACTCCAGGTTGAGGAGTTACTCGGAATGCTGCCAATATATCAGTATCCTTGGTTTGGTACTCAGGAGTATAATAAGTCAATTTGTACTCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGGGGGTGACAATAGCCCCTACGCTCTACCGTAGTTTTTAGCAGATAACCCCAATTTAGGTTTAATAGTACATCCCAACAGGGGACGACCATACTTGTTCAATTTATCTCTTTCAACTTGGATCCCATGAGGCGGACCTTGGAAAGTTTTAACATAAGCAGGAGGGATTCGCAGATCTTCCAGACGTAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATATTGGTAACGGAACCTTCTTCAAAAAGGTCTAAAGGGTAAGCTACATAAGCAATATATTGATCTTTTTCTCCAACAACACGCTCGATGCGGTAGCATCGCCCTTTGTAACGATCAAGACTGGTAAGTCCATCGGTCCATACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACCGCGGCCCCTGCTTCTTCAGGTGGAACTCCAGGTTGAGGAGTTACTCGGAATGCTGCCAATATATCAGTATCCTTGGTTTGGTACTCAGGAGTATAATAAGTCAATTTGTACTCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTGGGGGTGACAATA#+8D[cr!/;HV^ly!/9IWcp} $0=GTbo-:FOZft)6DR\ht!.9GV`lw+7EP]jx !+7EP\ju   " . : F S ^ i u    ' 3 > I V b o |   " . : E P ] i v    & 2 = I U ` l w    ( 5 A L X c o z  #.:DO[fq~'2=JU`ly+6BNZeq} &1=HS^ju%2>JVbny*6BN[fr} ".:EP\ht~ #/;GT`lv $0<HS_kv%2?JVakw&1=IUamx%1=GR^iu "+5>IT`kv#+8D[cr!/;HV^ly!/9IWcp} $0=GTbo-:FOZft)6DR\ht!.9GV`lw+7EP]jx !+7EP\ju   " . : F S ^ i u    ' 3 > I V b o |   " . : E P ] i v    & 2 = I U ` l w    ( 5 A L X c o z  #.:DO[fq~'2=JU`ly+6BNZeq} &1=HS^ju%2>JVbny*6BN[fr} ".:EP\ht~ #/;GT`lv $0<HS_kv%2?JVakw&1=IUamx%1=GR^iu "+5>IT`kv   $:::)4**64::*/>>//>#>>%868/>>>8/>>>;;>;;>>;>;&>;;;;>>>>>>>>;;;>>*>>>>>>>>;3>>>>>>;>>>>;>666>>>>;;>>>;>;>>>>>>>>>>;>>>>>>>>66>66;>>;>>>;>>>>6>>>;>>;>;;>>>>>>>;>>>>>>;;>>>>;>>>>>>>>>>>>>>;>>>>;>1>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>6>>6>>>>>>>>;>>;>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>;>>;;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>38>>>>>>>>>>>>>>>>>>>>>>>>>>'>>>>>>>>8>>>>>>>>>>>>>>>>>>>60>>*>>>>>>>>>>>4>>>>>6:::::::::65::66::::::::::::6::::46:::4::6:6-)      $:::)4**64::*/>>//>#>>%868/>>>8/>>>;;>;;>>;>;&>;;;;>>>>>>>>;;;>>*>>>>>>>>;3>>>>>>;>>>>;>666>>>>;;>>>;>;>>>>>>>>>>;>>>>>>>>66>66;>>;>>>;>>>>6>>>;>>;>;;>>>>>>>;>>>>>>;;>>>>;>>>>>>>>>>>>>>;>>>>;>1>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>6>>6>>>>>>>>;>>;>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>;>>;;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>38>>>>>>>>>>>>>>>>>>>>>>>>>>'>>>>>>>>8>>>>>>>>>>>>>>>>>>>60>>*>>>>>>>>>>>4>>>>>6:::::::::65::66::::::::::::6::::46:::4::6:6-)   X6_6K4B&_?X]<FJb $LJ_ cRC (K8 D?/ I0))2Vb O_)8]/ FSa2 0 FKZ CN-V!C>DK#bb9 8G=0.Wbd'NbZK9WF_+6EQO8<6?_;Q3PHM! NKHK1 a>!# I E>1UN4FN'U/F2!` V^V#B` NQPFJ9#5E#F7ad <MV^ / #5 WT@&@%, Rb2- DFK V T 0;)8A_@VLO$IP;]2_5SC:YY";I^0 P 8<=,XbHSAM>6<C,FR;TZ,C15I#-7`(3 [Sbb_ PWEA5%6SDK+@](5R#+,%X1^= [%Y,W#_dA#F@G":=%\S(_'2]9 ;VG0.F32[C)R*O8'%6*L&LH#RVU66E E5 ^_<)A-6F[2<&.MB )QR`C2V1&Z%@37*& 0-#$DBO??2&YJ("3cMF)_X3.M 5E\M/B&IW2+\?%?jdN grIYW$p556k & P H Viw p?}?hIB!%:sKsr,!d,>R^ HVD%eA2 55F N-6T2q!4(Z[[?1:^qpiI`$q7@$#a1Cn=vJia-{3q4cskr_vfUV#U?jU9KP56~zSRIr9O  L/!j]Z^r F` me"<e5 A[dCshHS5rk:%ZGFYs1J3;uFI=;&6.w=Z#T,Q[d i7gTlH<yRL1 BEODw,\C3 y-@T H0g^> -o5Hv}Yhx`VOCmGJ?9MC6*?4#!/                       <      (* #"".%,&- .%1'302'# !17(05(/&0 80, #),TP>OO,pI%?(VLCr?&RpD|P/W~.SPt(xfrPC(pPI}bJ1p!v_/u=qpP81pkxUT, x42",Lp# 2mS,p\8tqZx5RAXkI[ KUpepp dXYA'A(,ei!VVl6X8c8`pd]_pi Rpgp@8-3k p9"pbf ( p S|{`.q%pCC6srG"|h*KU.$2iJ7 BA56+ !Ok<,3 @&(%~#$'%'")B' $ #(#8(-6Q*"  # )C ):'F #%4/2#0"#+74 $ A$)!:%9'' %<!$/B3/"#& 3&%) 6!H1)-"0-9"!/200I/<<-*$&2&(&#H(+.*&2"%%.:-+?"$&13+* /%2,$"0#@!.!$)0 %,/=*@#'9)5DY'K:?,+/("5#6#2 &;) #%=%0*#+>C7*%08%++*4'/&S,!@7&..G,1/$-1L,9W)!&7,2@6 T3!*\,#;#C@Q5L(O%O% Gn 2RAAAAAACAATCTATAGCATAGAAAATCACATATCATTACCAAATTTGAATTATAACACCTGACCACCAAACACGGACACTCGTGAACCAAATCTCTCAAACCGCGAAGGTGGCGAGCCGCCGATCGAAGGGTGAAACGGCAGGAGCCAGAAAGACTCTCAGATCCTACAGACAGAGAGCGCGGAATGCAAAGCTTTCGGATTCACTTTCCACGGAAGGAGTTGTCATCGTAAGGGAAGGTTCCTCATCGGGGAAGTCGGGAAAGTGAGCCTCACGAGCTATAGAGTTATCCCCCTCTTCCACGAGTCTCTCTAATCAATATCATCTTTCAATGGGTAGAGGGAGAGAAGGGAGTTCATCCGTTCACTCAGCTATTCAAGGTACAAGAGGAGAATCATCAGATTTCGCTTTTTCTTAATAATGAAAATTTTCTTCAAGGCGAAGAGACAATCAGGAATCCGACCAGAGATGAGTATTCCCCGCGCCGCTGTCAAGAATATCACGAGTCGAGGTATTCTCCATCACTTCAGCAGCGTATTCCACACAAGCCCGAGTCTCTGGGTTTATGAGATTACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:34:38 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS@BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 8pTDATA 8pĐDATA 8p4DATA 8pDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~?qLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM>|+CP1RL>|&P1WD>|/P2AM>|8P2BA>>=3P2RL>|4;PBAS>>PBAS>>PCON>>$PCON>>"KPDMFTlPDMFPLOC>|PLOC>|SPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAg9FSPACSPACAg9FSVER4.0SVER @SVER,,/Scal@Scan@TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS@BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 8pTDATA 8pĐDATA 8p4DATA 8pDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabOUFVocODFWO_GATCFeat//OGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~?qLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM>|+CP1RL>|&P1WD>|/P2AM>|8P2BA>>=3P2RL>|4;PBAS>>PBAS>>PCON>>$PCON>>"KPDMFTlPDMFPLOC>|PLOC>|SPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAg9FSPACSPACAg9FSVER4.0SVER @SVER,,/Scal@Scan@TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`D5^s1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\fV9u>L0~Rj~z{FclrCk|7[Z%x]lswa:t"@>oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHzC)O`"]0n;(QXu|UP35US>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Ou`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0|+CP1RL>|&P1WD>|/P2AM>|8P2BA>>=3P2RL>|4;PBAS>>PBAS>>PCON>>$PCON>>"KPDMFTlPDMFPLOC>|PLOC>|SPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAg9FSPACSPACAg9FSVER4.0SVER @SVER,,/Scal@Scan@TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTR