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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 22a374dc8e204e4d88a4d25c0b746311Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################K1321_A5_Ardisia_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z~wttuuusqponmkifc`_^__^\XTOLJKMOQRQNJGEEFHJKJGC?;878:<==;853247:<>???@ACDEFGJNTZafjnt|0ARctrbRB0xgXLD@>??><:8665544469>DINPQPNKIHIJLMLHC=8679<>?>==?BEGGEA><=AGMSVXXVUTRPOMMQXds*dLpwQ uBuaSIB>;8767;@HR_n    jWG;0&%*-.,*&"     *A]>S\XJ4tW?, #6Xk43UT3v<@gp8KZ>-%!!"#%&''&#       ';X*^{V0%]wN@0+/+=yG&      Avja xH)              'a'HaW2"!&,/* Q/1;Y~-YpE% &.59972-&2nT~* X2\$ #*17::72+$  !#$#     $.7?DGFC<4,$                     AW: '1B)%E{(#$$$%(*,+("  "#! $'*++)&$#$%%! 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"(,./000-)# hk@vAAJAc KB.bcpKB_3730_POP7_BDTv3.mobtermGCTCAAAAGCTCTACGTAGTTTTTAGCGGATAATCCCAATTTAGGTTTAATAGTACATCCCAACAGTGGACGACCATACTTATTCAATTTATCTCTTTCAACTTGGATACCATGAGGCGGTCCTTGGAAAGTTTTAATATACGCAGTCGGGACTCGCAGATCTTCCAGACGTAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCCACGATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAAGGATAAGCTACATAAGCAATATATTGATTTTCTTCTCCAGCAACAGGCTCGATGTGGTAGCATCGCCCTTTGTAACGATCAAGGCTAGTAAGTCCATCGGTCCACACGGCTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCGGCCCCTGCTTCTTCGGGCGGAACTCCGGGTTGAGGAGTTACTCGGAATGCTGCCAAGATATCAGTATCCTTGGTTACATACTCAGGAGTATAATAAGTCAATTTGTAATCTTTAACACCAGCTTTGAATCCAGTACCTGCTTTAGTCTCTGTTTTGGGGTGACATGATACAAGCTCAAAAGCTCTACGTAGTTTTTAGCGGATAATCCCAATTTAGGTTTAATAGTACATCCCAACAGTGGACGACCATACTTATTCAATTTATCTCTTTCAACTTGGATACCATGAGGCGGTCCTTGGAAAGTTTTAATATACGCAGTCGGGACTCGCAGATCTTCCAGACGTAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCCACGATAGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAAGGATAAGCTACATAAGCAATATATTGATTTTCTTCTCCAGCAACAGGCTCGATGTGGTAGCATCGCCCTTTGTAACGATCAAGGCTAGTAAGTCCATCGGTCCACACGGCTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCGGCCCCTGCTTCTTCGGGCGGAACTCCGGGTTGAGGAGTTACTCGGAATGCTGCCAAGATATCAGTATCCTTGGTTACATACTCAGGAGTATAATAAGTCAATTTGTAATCTTTAACACCAGCTTTGAATCCAGTACCTGCTTTAGTCTCTGTTTTGGGGTGACATGATACAA $2=Uam $0>JTco~$.=LWcpz ,6CR^m{!.6AN[hr~(5?KUakx(5@KWbnz!-;HU_lw +8EP[iu    - 7 C N Z e q }  $ / ; G T _ k w    ' 2 ? 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L W c o {    + 6 B N Z f s }  # . : E Q \ h t '4?JVamz #/:FS_lx*6CO[gt'2=IUbo| ".:GR^ku*7CO[gt)4@KWdo} $0=JWbmy,8DO\ht'4ALYdo{ $0;GS`ly!,8DP[ht'2>GQ[gr|+1:     $$ '':  ::#::'>&!6&/88/1>>8*1>>/*8//>>>>;;>;;;;>>>>>>>>;'>>>;>>>>>>>>;3>>>>>>;>>>;;>;;>>>>>>>>>>;>;>>>>>>>>>>>>6>>>>6>>>>>;;>>;;>1;>>>>>>;;;>>>>*>>>>>>>>>>>>>>>;;>>>>>>>>6>>>>>>>>>>>>>+1*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>66>>>;>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>;>>8;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>6*>>6>>>>>8>>>>>>>>>>>>>4>>>>>>>>6>>>>>>>>>>::::::1:::::::::::::::16::::::::::#4::6::::::::6::::6-         $$ '':  ::#::'>&!6&/88/1>>8*1>>/*8//>>>>;;>;;;;>>>>>>>>;'>>>;>>>>>>>>;3>>>>>>;>>>;;>;;>>>>>>>>>>;>;>>>>>>>>>>>>6>>>>6>>>>>;;>>;;>1;>>>>>>;;;>>>>*>>>>>>>>>>>>>>>;;>>>>>>>>6>>>>>>>>>>>>>+1*>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>66>>>;>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>;>>8;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>6*>>6>>>>>8>>>>>>>>>>>>>4>>>>>>>>6>>>>>>>>>>::::::1:::::::::::::::16::::::::::#4::6::::::::6::::6-    P4T( L$(&*" _#S*P8-8_b(*(E1A<!^MN"1%0/ UPY" 6b.GO/5%HOF`bS F96YV)], *b]" Oa:^G!QIP/TF_c19?BN66;(1\E0L?X=I I11?% /%D8D4,b> M?RG6& 0 MU b7?UL[V "=]XVL*!(CTd] /B@$*SR0,7cCNM\,?6J-EYP+8 EZ@M.0$=GG[6[S5IaCI D/ EVdV:%T   U.Q[>Z31Q:M..5*:*CX<'XWE$ZZ1N]$K:7J=Z4?,LB;7&=<DX->[J=97dE\1C%a3 aO3-  ._M(,bB /5D"(U@4&PRXQKQ<.& LW306 \UU8E 2`c[ V VV/!PQ84RM"JP=I1_"4PG `7K; <*d2[:086FI- \$7HL\bYR*!L!B-B99\EC#X=J. "OPH^S@bK2<"H2VMcG.&C!!Q2$`bc2%RV]H2 DiDo ns ?lV= ht$vz7,wV !)vR_F ChD J~% . |(N k8hDwj<gG~I%TS R~565wXJS\e%V# 'xFlk ZU*PFqu(vXdjpuW{!A$>W\iFz 2S{>Io!nE r F"@mS {pqJ\=@ |91[`aU !=6,@bhS~;~:h]pbX3  B/){j|;W,I@Gz~E>T<YyX<   8Au+e p* {F-VBuQ+ctz,NHUv:9'< 77A\H$ (WXXA"]9kX,2[ M22n#PPZ Y y{y\x&2@ZzemedywY^WXhH(SA2?:1/F8 C*#'#!                       #' (2." *-# #-% !  (0)% '.+,B'F<!4,:'5J;;-.?'/9)-'.$/-(((((!,!!!L!!-wY*Bp>m616]Af(@"~*;--.:g /)sOh 0G:&M~KhcpPRx9@,5V!d@lSb#8yIIgR6F.W'rwpsG"~_* ldy8wRXtsD  ^C@dO,w6P:G}DSA}r8(dPj8,G7 |#HYcpY8) a`dOnpppppQf@TP  U_Q TU9f+uaOT%ew~$P"B*Ck7 :F&k%?'mK7+2:]!5<,D-F9 $'.4 +)!7/9A$F( 84&>)5+04T'#'!8*(A*#9"!!%#2,( )!  '=  !!6')%B3%@3 2/%!$5Q$?H)1(0&!.'R"F0/%))H>LQ3#='. N#30!("55G-I77&7"!2/$4#+*.)"/&8$>2/"4,: /B5*)').*#;(3; 1 '&-$!.3?-'I #"2(+!&8E\.I<" $8+#1!%8*1!.+"0."0f!&$0 0.2 !OY-'->C?")@(83I!4 #<)'%;&+ .H&1G..*(*DTk"$)D33!&-M1%,I/M'a\ @l'L= 2af#eAAAATTCCATCTATGCAGAAGCCATCGCATATTATTACCAGATATGAATTATAGCACCTGACCACCGAACACGGACCCTCCGGCACCAAATCTCGCCAACCACCCATACGGCGAGCCGCCGTTCGCAGGGAGAACTTCCACTCGCCGGCAAGTCTCTCAGGTCCTACAGACAGAGAGCGCGCAAAGCCAAGTTTTCCGACTCCATTTTCTCGAGAGGAGGTGTCGAGAGTACGTGAGCGTCCTCCTCGGGGAAGTCTGGAAGGGGCGCCTCACGAGCTATATAGTTACCCTCCTCTTCAGCGGGAAGCTCGACAAAAGAGCCTCTTTCCATAGGGAGAACGAAGGGTGGGTGTTCATCCCTTCTCGCTTCACTTCGAGCTACAAGAGGAGAATCAGGAGGGTGAGCTTTTTCTTCATAATTAATTATTTCTTCTAGCAGAAGAGATTCTCAGGAATCCTTCGAGACAGGACCATTCCTTGCGTCCTTGTCAAGAAGAGGATGAGTGGAAATATTATCCACGACTTCATCGGAGTATTCAGCATGAGGGAGAGTCTGTGGGGTTATGACATAATATACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:34:22 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XS<DATA ?XҔDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~} LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMBhP1RLBd7P1WDBm?P2AMBvGP2BABBzP2RLBqPBASBBTiPBASBBR'PCONBBaPCONBB_PDMFTlPDMFR PLOCB[/PLOCBVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAfTSPACRSPACAfTSVER4.0SVER ~SVER,,/Scal@Scan@TUBEA5Tmpr<UserphARphCHR"phDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XS<DATA ?XҔDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~} LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMBhP1RLBd7P1WDBm?P2AMBvGP2BABBzP2RLBqPBASBBTiPBASBBR'PCONBBaPCONBB_PDMFTlPDMFR PLOCB[/PLOCBVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAfTSPACRSPACAfTSVER4.0SVER ~SVER,,/Scal@Scan@TUBEA5Tmpr<UserphARphCHR"phDYbigphQLcphTRphTR"3Z2VW)$>ZHm? V<(?:134]3[16Qu5r`xC>2aZ!ly^A9Jz?gMBI+\$jn18A7y!H#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;{v3Ah-8^#~C=O\rHU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nX>zt %v1?{6W8:7F}wxSB#_{=pQ$+Sn0&'*#))MR]N=]TAO$Cs-1G@cJ-omMOxq6agI)Y_bO?C!.tk,8#V>Ax7cXF$hHzC)O`"]0n;(QXu|xh3P x>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Ou[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %> Ube1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&4&SG1T1M};<[!PkBV$603:m-(=hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;=AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?XTDATA ?XDATA ?XS<DATA ?XҔDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~} LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMBhP1RLBd7P1WDBm?P2AMBvGP2BABBzP2RLBqPBASBBTiPBASBBR'PCONBBaPCONBB_PDMFTlPDMFR PLOCB[/PLOCBVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAfTSPACRSPACAfTSVER4.0SVER ~SVER,,/Scal@Scan@TUBEA5Tmpr<UserphARphCHR"phDYbigphQLcphTRphTR